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The Control of Apple Maggots in Organic Orchards with GF-120 NF ...

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<strong>The</strong> <strong>Control</strong> <strong>of</strong> <strong>Apple</strong> <strong>Maggots</strong> <strong>in</strong> <strong>Organic</strong> <strong>Orchards</strong><br />

<strong>with</strong> <strong>GF</strong>-<strong>120</strong> <strong>NF</strong> Naturalyte Fruit Fly Bait<br />

Julia Reekie and Eric Specht<br />

Agriculture and Agri-Food Canada, Nova Scotia<br />

Kathryn Carter, Margaret <strong>Apple</strong>by and Hannah Fraser<br />

Ontario M<strong>in</strong>istry <strong>of</strong> Agriculture, Food and Rural Affairs<br />

Ken Wilson, Thornbury, Ontario<br />

L<strong>in</strong>dsay P<strong>in</strong>k, Markdale, Ontario


What is <strong>GF</strong>-<strong>120</strong>?<br />

• Sp<strong>in</strong>osad bait produced<br />

naturally by the<br />

fermentation <strong>of</strong> the soil<br />

act<strong>in</strong>omycete<br />

Saccharopolyspora<br />

sp<strong>in</strong>osa<br />

• Sp<strong>in</strong>osad is a mixture <strong>of</strong><br />

two most active naturally<br />

occurr<strong>in</strong>g metabolites<br />

(sp<strong>in</strong>osyns A and D)<br />

• <strong>GF</strong>-<strong>120</strong> is approved by the OMRI for use <strong>in</strong> organic<br />

production<br />

• It has been registered for use to control cherry fruit flies<br />

<strong>in</strong> cherries and blueberry maggots <strong>in</strong> blueberries<br />

• To date, it has not been registered for use <strong>in</strong> apples


Apply<strong>in</strong>g <strong>GF</strong>-<strong>120</strong><br />

<strong>GF</strong>-<strong>120</strong> has to be applied as<br />

soon as adult flies emerge<br />

• Rate=1.5 L/ha<br />

• Dilution 1:4 (<strong>GF</strong>-<strong>120</strong>: water)<br />

• Large droplet size (4-6 mm<br />

<strong>in</strong> diameter)<br />

• Uniform coverage is not<br />

critical<br />

• Repeat application 7 - 10<br />

days<br />

• Re-apply after a ra<strong>in</strong> event


<strong>GF</strong>-<strong>120</strong> Trials <strong>in</strong> 2007<br />

Results from 2007 trials: <strong>GF</strong>-<strong>120</strong> is as effective as<br />

Surround WP <strong>in</strong> the control <strong>of</strong> apple maggot <strong>in</strong> 5<br />

trials conducted <strong>in</strong> 2007<br />

One exception: At the “Home” orchard <strong>in</strong> Ontario, low<br />

rate <strong>of</strong> <strong>GF</strong>-<strong>120</strong> has significantly more <strong>in</strong>fested<br />

apples than Surround WP<br />

Currently <strong>GF</strong>-<strong>120</strong> is go<strong>in</strong>g through a label expansion<br />

for maggot control <strong>in</strong> apple crops


Objectives for 2008 trials<br />

To optimize the delivery <strong>of</strong> <strong>GF</strong>-<strong>120</strong> for adequate<br />

apple maggot control<br />

Experiment. 1 Temporal strategies us<strong>in</strong>g two<br />

application <strong>in</strong>tervals<br />

Experiment. 2 Spatial strategies practic<strong>in</strong>g perimeter<br />

spray or spray<strong>in</strong>g alternate rows<br />

Experiment. 3 Ref<strong>in</strong>e application methods for the<br />

maximum distribution <strong>of</strong> <strong>GF</strong>-<strong>120</strong> <strong>with</strong><strong>in</strong> tree canopies<br />

and to prune large-sized trees for better penetration<br />

<strong>of</strong> <strong>GF</strong>-<strong>120</strong> <strong>in</strong>to <strong>in</strong>ner tree canopies


Experiment 1 Temporal Strategies<br />

• A seedl<strong>in</strong>g mix research orchard <strong>in</strong><br />

Nova Scotia<br />

• 4 replications <strong>of</strong> 2 application<br />

<strong>in</strong>tervals (7-day and 14-day)<br />

• <strong>Control</strong> <strong>with</strong> no treatment (to<br />

<strong>in</strong>dicate pest pressure)<br />

• Label rate <strong>of</strong> <strong>GF</strong>-<strong>120</strong>: 1.5 L/ha<br />

Row<br />

Tree 13 12 11 10 9 8 7 6 5 4 3 2 1<br />

1 <strong>Control</strong> C C C Rep 4 T2 T2 T1 T1 T1 T1<br />

2 C T2 T2 T2 T2 T1 T1 T1 Rep 1<br />

3 C C C C T2 T1 T1 T1<br />

4 C T2 T2 T2 T2 T1 T1 T1 T1<br />

5 C C C C T2 T2 T2 T1 T1 T1 T1<br />

6 C T2 T2 T2 T1 T1 T1 T1<br />

7 C C C C C T2 T2 T2 T1 T1 T1 T1<br />

8 C C T2 T2 T1 T1<br />

9 C C C C T2 T2 T2 T2 T1 T1<br />

10 C C T2 T2 T2 T2 T1 T1 T1 T1<br />

11 C C C C C T2 T2 T2 T2 T1 T1 T1 T1<br />

12 C C T2 T2 T2 T2 T2 T2<br />

13 C C C C T2 T2 T2 T2 T2 T2 T2 T2<br />

14 C C T2 T2 T2 T2 T2 T2 T2<br />

15 C C C C T2 T2 T2 T2 T2 T2 T2<br />

16 C C T2 T2 T2 T2 T2 T2 T2 T2<br />

17 C C C C T2 T2 T2 T2 T2 T2 T2<br />

18 C C T2 T2 T2 T2 T2 T2<br />

19 C C C C T2 T2 T2 T2<br />

20 C C C T1 T1 Rep 3 T2 T2 Rep 2<br />

21 C C C C T1 T1 T1 T1 T2 T2<br />

22 C C C T1 T1 T1 T2 T2 T2<br />

23 C C C C C T1 T1 T1 T1 T2<br />

24 C C C C T1 T1 T1 T2 T2<br />

25 C C T1 T1 T1 T1 T2 T2 T2<br />

26 C C C C T1 T1 T1 T2 T2 T2<br />

27 Rep 4 T1 T1 T1 T1 T1 T1 T1 T2 T2 T2 T2<br />

28 T1 T1 T1 T1 T1 T1 T1 T1 T2 T2 T2 T2<br />

29 T1 T1 T1 T1 T1 T1 T1 T2 T2 T2 T2<br />

30 T1 T1 T1 T1 T1 T1 T2 T2 T2 T2<br />

31 T1 T1 T2 T2 T2 T2 T1 T1 T1<br />

32 T1 T1 T1 T2 T2 T2 T1 T1 T1 T1<br />

33 T1 T1 T1 T1 T2 T2 T2 T1 T1 T1 T1<br />

34 T1 T1 T1 T2 T2 T2 T1 T1 T1 T1<br />

35 T1 T1 T1 T1 T2 T2 T2 T1 T1<br />

36 T1 T1 T2 T2 T2 T1 T1 T1<br />

37 T1 T1 T1 T2 T2 T2 T2 T1 T1 T1<br />

38 T1 T1 T2 T1 T1<br />

39 T1 T1 T1 T1 T2 T2 T2 T1 T1 T1<br />

40 T1 T2 T1 T1 T1<br />

41 T1 T1 T2 T2 T2 T1<br />

42 T2<br />

43 T2 T2 T2<br />

44 T2 T2<br />

45 T2 T2 T2<br />

46 T2<br />

47 T2 T2<br />

48 T2<br />

49 T2<br />

50


Experiment 2 Spatial Strategies<br />

• ‘McIntosh’ and ‘Cortland’ research orchards <strong>in</strong> Nova Scotia<br />

• 4 replications <strong>of</strong> 3 treatments: perimeter spray, spray alternate<br />

rows and spray all rows<br />

• <strong>Control</strong> <strong>with</strong> no treatment (to <strong>in</strong>dicate pest pressure)<br />

• Label rate <strong>of</strong> <strong>GF</strong>-<strong>120</strong>: 1.5 L/ha<br />

WEST<br />

NORTH<br />

Tree Blk 4 Blk 3 Blk 2 Blk 1<br />

40 M M M M C C M 40<br />

39 C C M M C C M M C C M M C C M 39<br />

38 C C M M C C M M C C M M C C M M C C M M 38<br />

37 C C M M C C M M C C M M C C M M C C M M 37<br />

36 C C M M C C M M C C M M C C M M C C M M 36<br />

35 C C M M C C M M C C M M C C M M C C M M 35<br />

34 C C M M C C M M C C M M C C M M C C M M 34<br />

33 C C M M C C M M C C M M C C M M C C M M 33<br />

32 C C M M C C M M C C M M C C M M C C M M 32<br />

31 C C M M C C M M C C M M C C M M C C M M 31<br />

30 C C M M C C M M C C M M C C M M C C M M 30<br />

29 C C M M C C M M C C M M C C M M C C M M 29<br />

28 C C M M C C M M C C M M C C M M C C M M 28<br />

27 C C M M C C M M C C M M C C M M C C M M 27<br />

26 C C M M C C M M C C M M C C M M C C M M 26<br />

25 C C M M C C M M C C M M C C M M C C M M 25<br />

24 C C M M C C M M C C M M C C M M C C M M 24<br />

23 C C M M C C M M C C M M C C M M C C M M 23<br />

22 C C M M C C M M C C M M C C M M C C M M 22<br />

21 C C M M C C M M C C M M C C M M C C M M 21<br />

19 C C M M C C M M C C M M C C M M C C M M 19<br />

18 C C M M C C M M C C M M C C M M C C M M 18<br />

17 C C M M C C M M C C M M C C M M C C M M 17<br />

16 C C M M C C M M C C M M C C M M C C M M 16<br />

15 C C M M C C M M C C M M C C M M C C M M 15<br />

14 C C M M C C M M C C M M C C M M C C M M 14<br />

13 C C M M C C M M C C M M C C M M C C M M 13<br />

12 C C M M C C M M C C M M C C M M C C M M 12<br />

11 C C M M C C M M C C M M C C M M C C M M 11<br />

10 C C M M C C M M C C M M C C M M C C M M 10<br />

9 C C M M C C M M C C M M C C M M C C M M 9<br />

8 C C M M C C M M C C M M C C M M C C M M 8<br />

7 C C M M C C M M C C M M C C M M C C M M 7<br />

6 C C M M C C M M C C M M C C M M C C M M 6<br />

5 C C M M C C M M C C M M C C M M C C M M 5<br />

4 C C M M C C M M C C M M C C M M C C M M 4<br />

3 C C M M C C M M C C M M C C M M C C M M 3<br />

2 C C M M C C M M C C M M C C M M C C M M 2<br />

1 C C M M C C M M C C M M C C M M C C M M 1<br />

ROW 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1


Experiment 3 Ref<strong>in</strong>e Application Methods<br />

• Commercial ‘McIntosh’ orchard <strong>in</strong> Ontario<br />

• 4 replications <strong>of</strong> 6 treatments:<br />

1. Low rate <strong>GF</strong>-<strong>120</strong> (1.5 L/ha) applied <strong>with</strong> one pass to trees<br />

received standard prun<strong>in</strong>g<br />

2. High rate <strong>GF</strong>-<strong>120</strong> (2.25 L/ha) applied <strong>with</strong> one pass to trees<br />

received standard prun<strong>in</strong>g<br />

3. Low rate <strong>GF</strong>-<strong>120</strong> (1.5 L/ha) applied <strong>with</strong> two passes to trees<br />

received standard prun<strong>in</strong>g<br />

4. High rate <strong>GF</strong>-<strong>120</strong> (2.25 L/ha) applied <strong>with</strong> two passes to trees<br />

received standard prun<strong>in</strong>g<br />

5. Low rate <strong>GF</strong>-<strong>120</strong> (1.5 L/ha) applied <strong>with</strong> one pass to trees<br />

received extra prun<strong>in</strong>g<br />

6. High rate <strong>GF</strong>-<strong>120</strong> (2.25 L/ha) applied <strong>with</strong> one pass to trees<br />

received extra prun<strong>in</strong>g<br />

• <strong>Control</strong> <strong>with</strong> no treatment (to <strong>in</strong>dicate pest pressure)


Experiment 3<br />

Ref<strong>in</strong>e Application Methods<br />

(cont<strong>in</strong>ued)


Assessment<br />

• At harvest, 6-10 trees from each<br />

experimental plot were sampled<br />

and from each tree, 20 apples<br />

were randomly collected<br />

• For each orchard, 20 apples from<br />

16 control trees were randomly<br />

collected<br />

• Fruits were <strong>in</strong>cubated at room<br />

temperature for 2 weeks before the<br />

assessment <strong>of</strong> apple maggot<br />

damage<br />

• Every fruit was cut <strong>in</strong>to quarters<br />

and exam<strong>in</strong>ed for apple maggot<br />

<strong>in</strong>jury


Nova Scotia Results- % <strong>Apple</strong> Maggot Injuries<br />

<strong>Orchards</strong><br />

Treatments ‘McIntosh’ ‘Cortland’ Seed<strong>in</strong>gs<br />

7-day Spray -- -- 3.30 a<br />

14-day Spray -- -- 10.20 b<br />

Perimeter Spray 0.20 a 0.40 a --<br />

Alternate Rows 1.25 a 1.25 a --<br />

All Rows 0.65 a 2.00 a --<br />

<strong>Control</strong> 35.30 30.6 68.8


Ontario Results- % <strong>Apple</strong> Maggot Injuries<br />

Rate Pass Prune ‘McIntosh’<br />

Low 1 standard 8.00 a<br />

High 1 standard 5.08 b<br />

Low 2 standard 2.78 c<br />

High 2 standard 5.70 b<br />

Low 1 extra 3.78 c<br />

High 1 extra 2.28 c<br />

<strong>Control</strong> 42.00


Conclusion<br />

• <strong>GF</strong>-<strong>120</strong> should be applied every 7-10 days for adequate<br />

apple maggot control (especially when pest pressure is<br />

high)<br />

• It is possible to spray only the perimeter <strong>of</strong> the orchard or<br />

spray alternate rows for apple maggot control (provid<strong>in</strong>g<br />

there is no <strong>in</strong>ternal pest <strong>in</strong>festation)<br />

• Good penetration and even distribution <strong>of</strong> <strong>GF</strong>-<strong>120</strong> <strong>with</strong><strong>in</strong><br />

tree canopies are crucial for apple maggot control (<strong>in</strong> largesized<br />

apple trees)


Acknowledgement<br />

• Fund<strong>in</strong>g obta<strong>in</strong>ed from Pest Management Centre -<br />

Biopesticides Initiatives<br />

• Dow AgroSciences donated <strong>GF</strong>-<strong>120</strong> <strong>NF</strong> Naturalyte<br />

fruit fly bait for field trials<br />

• Frank Hewgill <strong>Orchards</strong> Ltd., Ontario<br />

• Robert Gardner <strong>Orchards</strong>, Ontario<br />

• Boates Orchard, Nova Scotia<br />

• Colleagues, friends and families who helped <strong>with</strong><br />

this project

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