peqGOLD Universal Agarose Tablets

Data sheet
peqGOLD Universal
Agarose Tablets
Cat. No.: 35-7010 / 20 / 30
Lot No.:
…………..
Introduction:
peqGOLD Universal Agarose Tablets are ideally suited for use as
standard agarose for analytical as well as preparative nucleic acid
electrophoresis of fragments from 50 bp to 50 kbp. For use in routine
analysis of DNA, Universal Agarose is supplied in convenient and
waste saving 0.5 g tablets.
Separation range:
DNA: approx. 0.05 kbp – 50 kbp
RNA: approx. 0.30 kb – 20 kb
Cat. No.:
35-7010 Universal-Agarose Tablets, 50 g
35-7020 Universal-Agarose Tablets, 250 g
35-7030 Universal-Agarose Tablets, 500 g
Quality:
• 'Molecular Biology Grade'
• Certified free of DNases and RNases
• No DNA binding
• High lot-to-lot consistency
Characteristics:
• Easy to handle, pre-weighed tablets
• Reduces waste and avoids weighing-out
• Dust free formulation
• Highly reproducible, just count the tablets
Analytical specifications:
• Gelling temperature: ≤ 39 °C
• Melting temperature: ≤ 90 °C
• Electroendosmosis: ≤ 0.120
• Gel strength (1.5 %): ≥ 1200 g / cm 2
• Sulphate content: ≤ 0.10 %
• Water content: ≤ 10.0 %
Safety instructions:
Always wear eye protection when preparing agarose gel solutions
and protect yourself and others against boiling liquids. Refer to the
material safety data sheet for further safety and handling instructions.
Preparing agarose:
1. Pour buffer (approx. 90 % of final volume) into an appropriate
flask that can accommodate up to four times the final gel volume
and add a magnetic stir bar.
2. Add the required number of agarose tablets and incubate until
the tables are completely dissolved (typically after 5 minutes).
Note: Gently swirling and the use of prewarmed buffer (50 °C
to 60 °C) will reduce incubation time! If the buffer is too hot,
the tablet will not melt properly!
Temperature
Time
4 °C 13 min.
RT (approx. 25 °C)
5 min.
40 °C 5 min.
50 °C 3 min.
60 °C 3 min.
70 °C 4 min.
80 °C 6 min.
90 °C Tablet will not melt!
3. Remove magnetic stir bar.
4. Add remaining buffer up to the desired final volume.
5. Weigh and record the weight of the flask prior to heating. Heat
for 1 – 2 minutes in a microwave oven (600 Watt). Gently swirl
the flask to mix the solution. Warning: Due to microwave
heating, there may be a delay in the liquid boiling!
6. Using the microwave oven, heat in short bursts of 5 –
10 seconds or until the solution is boiling, with breaks of 10 –
15 seconds between heating phases to disperse bubbles by
gently swirling the flask. Beware of hot glass ware and liquid.
Continue until the agarose is completely dissolved.
7. Again, weigh the flask and top up lost volume with warm,
deionized water. Gently swirl the flask to ensure complete
mixing.
8. Let the solution cool down at room temperature for 15 –
20 minutes or until a gel temperature of 50 – 60 °C is reached.
(Alternatively put the solution in a 55 °C water bath for at least
30 minutes.)
Note:
For preparing 100 ml of 1% agarose gel solution use 1 g agarose (2
0.5 g tablets) in 100 ml appropriate electrophoresis buffer.
• Manufactured and quality-controlled in accordance with
ISO 9001:2000
• Shipment at ambient temperatures
• Storage at room temperature
Peqlab_V0511_E
info@peqlab.com · www.peqlab.com

peqGOLD Universal Agarose Tablets
The PEQLAB electrophoresis range also includes:
- PerfectBlue Horizontal Mini Gel Systems
- PerfectBlue Horizontal Maxi Gel Systems
- PerfectBlue Vertical Double Gel Systems
- Power Supplies
- Marker (RNA and DNA Ladders, DNA-Sizer)
You already use peqGOLD Universal Agarose Tablets, but do you know our broad range of specialised agaroses for various
applications in nucleic acid separation?
Product Application Separation range Quantity Cat. No.
peqGOLD Universal Agarose Standard agarose for universal applications 0.05 – 50 kbp 100 g 35-1010
500 g 35-1020
1000 g 35-1030
peqGOLD 'Low Melt' Agarose Specialist agarose melting at lower
0.08 – 20 kbp 25 g 35-2010
temperatures and suitable for 'in gel'
100 g 35-2020
techniques 250 g 35-2030
peqGOLD MoSieve Agarose
MS-500
peqGOLD MoSieve Agarose
MS-1000
peqGOLD MegaBase Agarose
peqGOLD Pulsed Field Agarose
peqGOLD Universal Agarose
Tablets
Specialist agarose for separating small nucleic 10 – 1000 bp 25 g 35-3010
acid fragments with low electro-endosmosis
100 g 35-3020
250 g 35-3030
Specialist agarose for separating small nucleic 50 – 2000 bp 25 g 35-4010
acid fragments between 50 bp and 2000 bp
100 g 35-4020
and high gel strength for easy handling 250 g 35-4030
Agarose for separating large nucleic acid 0.2 – 50 kbp 25 g 35-5010
fragments
100 g 35-5020
250 g 35-5030
Specialist agarose suitable for especially long 1 kbp – 5 mbp 25 g 35-6010
DNA molecules and whole chromosomes by
100 g 35-6020
'Pulsed Field' Gel Electrophoresis 250 g 35-6030
Standard agarose provided as 0.5 g tablets 0.05 – 50 kbp 50 g 35-7010
for convenient preparation
250 g 35-7020
500 g 35-7030
Peqlab_V0511_E
info@peqlab.com · www.peqlab.com