AlphaLISA Assay Development Guide - PerkinElmer

Table III: Microplates
Assay
Format
96
384
1536
Microplate Catalog # § well
Total
volume
Recommendations
OptiPlate-96 6005290 400 µL Ideal for assay volume ≥ 50 µL
½ AreaPlate-96 6005560 180 µL
Ideal for assay volume of 50 µL in
96-well format
ProxiPlate-96 6006290 100 µL Not recommended
OptiPlate-384 6007290 105 µL
AlphaPlate-384 6005350 105 µL
ProxiPlate-384 6008280 30 µL
AlphaPlate-1536 6004350 12 µL
OptiPlate-1536 6004290 12 µL
§
For 50 plates; refer to PerkinElmer, Inc. website for other quantities
Good for assays of 25-50 µL in
384-well format
Ideal for high-count assays of 25-
50 µL in 384-well format (lower
cross-talk than OptiPlate-384)
Good for assays of 10-25 µL in
384-well format
Ideal for assays of 5-10 µL in
1536-well format (lower cross-talk
than OptiPlate-1536)
Not recommended for AlphaLISA
due to higher cross-talk
The protocols included in this manual are for OptiPlate-384 microplates.
Other plate types listed in Table III may be used with the appropriate assay
volume.
Assays must be read using an AlphaScreen-compatible reader such as all
EnVision ® multilabel plate readers with an AlphaScreen module, the Fusion-
Alpha multilabel reader or the AlphaQuest ® original AlphaScreen reader
(PerkinElmer, Inc.). The normal AlphaScreen settings should be used.
II.
INTENDED USE
The AlphaLISA Acceptor beads are intended to perform biomarker
detection (referred in this guide as analyte), protein-protein
interaction, intracellular target detection assays or any other
biological detection assay. AlphaLISA is for research purposes only
and not for use in any diagnostic procedures.
- 6 -