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Figure 3. Programming of the Cells using ‘CONCATENATE’ function to merge many text strings from different cells into single output cell. Conditional Formatting of the cells Conditional Formatting offers and easy way to apply formats that stay dormant until the values in the highlighted cells achieve a specified state as given in Figure 4. Here a tool was used to highlight cells with a certain color depending on cell value. The series of steps are as follows: The cells to be highlighted depending upon the condition were selected The option “Conditional Formatting” and select “New Rule” was clicked on The second option “Format only cells that contain” was selected and “specific text” in the dropdown box under “Edit the rule description” was subsequently selected and the value was entered. “Format” was clicked and different options such as number, font, border, fill etc., were used “Ok” was clicked to allow conditional formatting. On conditional formatting, different parameters are represented in different ways such as: The different cells containing PBS and RPMI are shown in different shades Cells with conditioned medium are shown by double underlining 4
Figure 4. Conditional Formatting of the programmed cells was used to change parameters in the format of the template to distinguish between different conditions in the cell culture plate Representation of analysed data In studies that involve series of experiments to standardize the 3D cell culture conditions, various variables and combinations of the variables have to be observed, data recorded and analyzed. For this purpose, a single template to analyze and represent all the parameters to record the results was developed. The parameters include concentration of agarose, culture surface area, types of culture medium, culture medium volumes, presence or absence of conditioned medium and various seeding densities. Thus the result entered in a single box is a representative of a combination of all these parameters as shown in Table 1. CONCENTRATION OF AGAROSE CM 0.25% 0.5% 0.75% +/- 6 12 24 6 12 24 6 12 24 PBS + 1 ml - + 750 µl - + 500 µl - + 250 µl - V O L U M E + 1 ml - RPMI + 750 µl - + 500 µl - + 250 µl - +/- X Y X Y X Y X Y X Y X Y X Y X Y X Y SEEDING DENSITIES Table 1. The template provides for various parameters including concentration of agarose, medium of cell suspension, volume of medium added, presence or absence of conditioned medium (CM) and seeding densities per well in a 6 well, 12 well & 24 well plate. 5
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Page 3: Figure 1. Data entry into excel cel

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