Polyacrylamide Gel Electrophoresis (PAGE) of Proteins

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• When making polyacrylamide gels, the ratio of crosslinking agent to acrylamide is a critical factor as it determines the pore size of the gel and influences it’s mechanical properties! • %T represents the total concentration of monomer (acrylamide + Bis) expressed in grams per 100 ml (weight per volume %) • %C is the percentage (by weight) of total monomer T which is due to the cross-linking agent (Bis) • The concentration of acrylamide used determines the average polymer chain length while the Bis concentration determines the extent of cross-link formation. Important in determining such physical properties of the gel as density, elasticity, mechanical strength, and pore size.
• Grinding of seeds (bulk or single seed) – Mortar and pestle – Coffee grinder – Udy mill – Pliers method –Etc. Protein Extraction
Page 1 and 2: Polyacrylamide Gel Electrophoresis
Page 5 and 6: Proteins are linear polymers of ami
Page 7 and 8: Why Use PAGE Protein Electrophoresi
Page 9 and 10: Types of PAGE Gels Many types exist
Page 11: Native PAGE - continuous • Separa
Page 16 and 17: SDS PAGE - discontinuous • Has th
Page 18 and 19: Sample wells Stacking gel. (Has lar
Page 20 and 21: • Polyacrylamide gels are prepare
Page 24: • Extraction buffers - Ethylene g
Page 27 and 28: Pouring the gel Inserting the comb
Page 29: Running the Gel • If using a reci
Page 32 and 33: • After running - disassemble gel
Page 35 and 36: Gel Evaluation and Documentation 1.
Page 37 and 38: XXXXX- Spelt Spelt 1 Spelt 2 2pelt
Page 39 and 40: R Strain STL #4448 Golden German R
Page 41 and 42: Hycrest Kirk STL #3335 Fairway Cres
Page 43 and 44: Stored in zip lock bag with water.
Page 45 and 46: Gel dried between two sheets of cel
Page 47 and 48: Cracked gel
Page 49: Missing gel piece.

Polyacrylamide Gel Electrophoresis (PAGE) of Proteins

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