Polyacrylamide Gel Electrophoresis (PAGE) of Proteins

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Montcalm Montcalm Red Hawk Red Hawk Montcalm Montcalm Red Hawk Red Hawk Kidney Beans SDSU Seed Testing Lab PAGE Gel #3-057 06/10/03 Note: 2 different types of extract were used on a PAGE gel: IEF and Urea. Both showed the same results. See bands for primary areas of differences. IEF Extraction (2-chloroethanol:H 2 O) Urea Extraction (2-chloroethanol: 2-mercaptoethanol)
Stored in zip lock bag with water. Will store for a couple of months.
Page 1 and 2: Polyacrylamide Gel Electrophoresis
Page 5 and 6: Proteins are linear polymers of ami
Page 7 and 8: Why Use PAGE Protein Electrophoresi
Page 9 and 10: Types of PAGE Gels Many types exist
Page 11: Native PAGE - continuous • Separa
Page 16 and 17: SDS PAGE - discontinuous • Has th
Page 18 and 19: Sample wells Stacking gel. (Has lar
Page 20 and 21: • Polyacrylamide gels are prepare
Page 22 and 23: • When making polyacrylamide gels
Page 24: • Extraction buffers - Ethylene g
Page 27 and 28: Pouring the gel Inserting the comb
Page 29: Running the Gel • If using a reci
Page 32 and 33: • After running - disassemble gel
Page 35 and 36: Gel Evaluation and Documentation 1.
Page 37 and 38: XXXXX- Spelt Spelt 1 Spelt 2 2pelt
Page 39 and 40: R Strain STL #4448 Golden German R
Page 41: Hycrest Kirk STL #3335 Fairway Cres
Page 45 and 46: Gel dried between two sheets of cel
Page 47 and 48: Cracked gel
Page 49: Missing gel piece.

Polyacrylamide Gel Electrophoresis (PAGE) of Proteins

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