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Preventing Mold-Related Problems in the Indoor Workplace - Seton ...

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■ After a cleanup, to show that <strong>the</strong> types and concentrations<br />

of mold <strong>in</strong> <strong>the</strong> area are similar to background levels (17).<br />

Sampl<strong>in</strong>g for mold, mold spores, mycotox<strong>in</strong>s, and o<strong>the</strong>r<br />

bioaerosols are not part of a rout<strong>in</strong>e build<strong>in</strong>g evaluation.<br />

<strong>Mold</strong> sampl<strong>in</strong>g strategies<br />

Sampl<strong>in</strong>g and analysis of mold are complex and can become<br />

expensive. There is a lack of standard procedures for sampl<strong>in</strong>g and<br />

analysis (18). Sampl<strong>in</strong>g should be undertaken only after careful<br />

del<strong>in</strong>eation of <strong>the</strong> sampl<strong>in</strong>g goals. For assistance with mold<br />

sampl<strong>in</strong>g, consult an experienced health and safety professional.<br />

Health and safety professionals, work<strong>in</strong>g closely with an accredited<br />

environmental microbiology laboratory, can determ<strong>in</strong>e and document<br />

<strong>the</strong> details concern<strong>in</strong>g <strong>the</strong> necessary sampl<strong>in</strong>g strategy,<br />

<strong>in</strong>clud<strong>in</strong>g when and where to sample. Standardized methods, such<br />

as ACGIH, AIHA, NIOSH and OSHA methods, should be followed<br />

where available. Accredited laboratories that participate <strong>in</strong> <strong>the</strong><br />

AIHA Environmental Microbiology Proficiency Analytical Test<strong>in</strong>g<br />

(EMPAT) Program are listed on <strong>the</strong> AIHA website at http://www.aiha.org/<br />

LaboratoryServices/html/empat1.htm.<br />

The mean<strong>in</strong>g of mold sampl<strong>in</strong>g results that are <strong>in</strong><br />

CFU/m 2 and CFU/m 3<br />

Sampl<strong>in</strong>g results for viable (liv<strong>in</strong>g) microorganisms are presented<br />

as concentrations, and <strong>the</strong> units used will vary depend<strong>in</strong>g<br />

on <strong>the</strong> sample collection methods. Air sampl<strong>in</strong>g results are<br />

reported as colony-form<strong>in</strong>g units per cubic meter of air (CFU/m 3 ).<br />

Specialized sampl<strong>in</strong>g is reported <strong>in</strong> terms of <strong>the</strong> entity collected,<br />

i.e., if only spores were sampled, <strong>the</strong> results would be reported<br />

as spores/m 3 (19). Bulk samples may be reported as colony-form<strong>in</strong>g<br />

units per gram (CFU/g) of dust or material or colony-form<strong>in</strong>g units<br />

per milliliter (CFU/ml) of stagnant water or slime (20). Wipe sample<br />

results are reported as colony-form<strong>in</strong>g units per surface area such<br />

as CFU/m 2 or CFU/ft 2 . These units represent <strong>the</strong> culturable<br />

portion of mold concentrations only and do not quantify <strong>the</strong><br />

fragments and by-products of mold that may also exist.<br />

14

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