Hydrophobic Interaction Chromatography - Sigma-Aldrich

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Sample Preparation for Mass Spectrometry Hydrophobic Interaction Chromatography Sigma-Aldrich ® offers four novel technologies for mass spec sample preparation: Trypsin digestion columns, C18 cleanup tips, oligosaccharide purification tips and phosphopeptide enrichment columns. Description Cat. No. Trypsin Spin Columns, Proteomics Grade TT0010 For 15 minute digestion of protein samples prior to LC-MS. Typsin is covalently immobilized to eliminate autodigestion and carryover. Column capacity 100 µL (10-100 µg protein) Trypsin from Porcine Pancreas, Proteomics Grade, Dimethylated T6567 ProteoMass Guanidination Kit For improving MALDI-MS sensitivity and increasing sequence coverage MS0100 Supel-Tips C18 Pipette Tips For reverse-phase concentration or cleanup for small samples of macromolecules, typically peptides prior to mass spectrometry analysis. 5-20 µg binding capacity. 10 µL working volume. TPSC18 NEW! Supel-Tips Carbon Pipette Tips 54227-U Designed to extract, concentrate, and/or purify oligosaccharides. A graphitized polymer carbon adsorbent bed acts as a solid phase extraction medium to adsorb oligosaccharides and other macromolecules containing sugar moieties. Can be used for the sample preparation/cleanup prior to HPLC, LC-MS, MALDI MS, and other analytical techniques. Sample volume 0.5-10 μL NEW! PhosphoProfile TM Phosphopeptide Enrichment Kit PP0410 Analysis of phosphopeptides from tryptic protein digests is a powerful tool for characterization and identification of phosphorylation sites. A combination of low intrinsic abundance, inefficient ionization, and/or signal suppression of most phosphopeptides may limit or even prevent detection, unless the phosphopeptide content is significantly enriched prior to analysis. This kit conveniently includes all materials needed to enrich phosphopeptides from digested samples in a robust and unbiased manner. The phosphopeptides are captured by a novel nitriloacetic acid (NTA) analog Ga 3+ chelate on 20 micron diameter silica with a pore size of 1,000 Angstroms. The matrix is packed into spin columns for easy, microscale affinity phosphopeptide capture. 36 ORDER: 800-325-3010 TECHNICAL SERVICE: 800-325-5832 sigma.com
Standardize! Epitope Tags in Protein Research With Sigma, standardize your proteomics research Verify and challenge your mass specrometry strategy with Sigma’s new Proteomics Dynamic Range Standard (UPS2). This standard is a complex, well-defined, well-characterized reference standard for mass spectrometry. An enhancement of Sigma’s original Universal Proteomics Standard (UPS1), this complex mixture of 48 human proteins has been formulated as a dynamic range of concentrations, from 0.5 fmoles – 50 pmoles. n Determine the limit of detection for your analytical strategy n Troubleshoot and optimize your analytical protocol n Confirm system suitability before analyzing critical samples n Normalize analytical results day-to-day or lab-to-lab To get your standard, visit sigma.com/ups Our Innovation, Your Research — Shaping the Future of Life Science sigma-aldrich.com
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