Plant Breeding & Genetics - Higher Education Commission
Plant Breeding & Genetics - Higher Education Commission
Plant Breeding & Genetics - Higher Education Commission
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elationship between cane yield and sugar recovery. Strategies for genetic<br />
improvement in sugar crops. Genetic determination of quality parameters and<br />
their utilization. Role of biotechnology in sugar crops improvement.<br />
Practical<br />
Estimation of brix value of sugarcane plant. Collection of data for various<br />
plant traits in sugar crops and their genetic analysis. Visit to research<br />
institutes and sugar industries.<br />
Books Recommended<br />
1. Henry, R. J. and C. Kole. 2010. <strong>Genetics</strong>, Genomics and <strong>Breeding</strong> of<br />
Sugarcane. Taylor and Francis, London, UK.<br />
2. Malik, K. B. 2009. Cane and Sugar Production. Punjab Agriculture<br />
Research Board, Lahore, Pakistan.<br />
3. Draycott, A. P. 2006. Sugar beet. Blackwell Publishing Ltd., Oxford, UK.<br />
4. Sleper, D. A. and J.M. Poehlman. 2006. <strong>Breeding</strong> Field Crops. 5th ed.<br />
Iowa State University Press, Ames, Iowa, USA.<br />
World Wide Web<br />
1. http://www.amazon.com/Sugarcane-World-Agriculture-<br />
GlynJames/dp/063205476X/ref=pd_bxgy_b_img_b<br />
PBG 713 Genetic Engineering in <strong>Plant</strong>s 3(2-1)<br />
Theory<br />
Molecular approaches in gene manipulation. Techniques for locating gene.<br />
Importance of gene cloning in research and industry. Cloning vehicle.<br />
Plasmids and bacteriophages, extraction and purification of DNA. Restriction<br />
endonucleases and ligases. DNA Probing, Southern and Northern blotting,<br />
PCR-amplification of DNA; cDNA- synthesis. Introduction of DNA into living<br />
cells. Selection for Recombinants; Cloning Vectors for E. coli and higher<br />
plants. Identification and selection of cloned gene. Formation of transgenic<br />
plants. Benefits and problems related with transgenic plants. Genetically<br />
modified plants and bioethical concerns. Bioinformatics.<br />
Practical<br />
Extraction of genomic DNA, purification of DNA, restriction mapping. Genetic<br />
transformation of bacteria and plants. Electrophoresis. Visit to National<br />
Institutions working in Genetic Engineering and Biotechnology.<br />
Books Recommended<br />
1. Slater, A., N. W. Scott and M. R. Fowler. 2008. <strong>Plant</strong> Biotechnology: the<br />
genetic manipulation of plants. Oxford University Press, Oxford, UK.<br />
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