CURRICULAM VITAE - Australasian Plant Pathology Society

Biotechnological Methods for Crop Disease Management. Annamalai University, Annamali
Nagar, India, March 3-4, 2006.
10. Surendiran, G., Srinivasan, K. and Mathivanan, N. 2006. Antioxidant activity of Morinda
pubescens. National Symposium on Molecular Basis of Disease Management. University of
Madras, Chennai, India. March 13-14, 2006.
Technical proficiency in
Microbiological skills - Isolation, identification and maintenance of microorganisms (Enrichment
culture technique) and Electron microscopical analysis.
Analytical technical skills - Enzyme assay (Quantitative & Plate assay), Extraction of bioactive
compounds from microorganisms, Purification of secondary metabolites from microbial strains by
chromatographic techniques (Column, Thin layer, HPLC FPLC and Gas chromatographic
techniques) and Estimation of growth hormones from plants.
Plant pathology technical skills - Biological control of plant pathogens e.g., bacteria, fungi and
virus, Induced systemic resistance enzyme assays, Systemic acquired resistance enzyme assays,
Formulation of biocontrol agents (powder and liquid formulations), Pathogenicity assay,
Glasshouse and Field trials, Plant tissue culture, Plant transformation and plant regeneration,
Virus maintenance in tissue culture calli, Protoplast isolation, regeneration and fusion - fungi &
plants.
Serological diagnostic methods - Antibody production, Double immuno diffusion test, Enzyme
linked immune sorbent assay and Western blotting.
Molecular biological techniques - Isolation and purification of nucleic acids (DNA and RNA from
bacteria, fungi, actinomycetes and plants), Agarose gel electrophoresis, Polymerase chain
reaction and Reverse transcriptase PCR techniques, DNA restriction analysis, Ligation, Cloning,
Real time PCR, RAPD and RFLP analyses, Transformation and Electroporation, Gene expression,
cDNA systhesis, qRT-PCR, Micro-array analysis, Sequencing analysis and Poly acryl amide gel
electrophoresis (Native and SDS-PAGE).
Bioinformatics tools - NCBI database, RASMOL, DNA Baser, MEGA, UPGMA, Phylogenetic analysis
(neighbour joining and Maximum Parasimony), Nucleic acid and Protein sequencing (proteins and
nucleic acids BLAST, pair wise and multiple sequence alignments), Clustawl analysis and TAIR.
Statistical knowledge - AGRES, ANOVA & SPSS statistical analysis for biological data.
Computer knowledge - Basic, Microsoft Office (MS-word, power point, excel), photo shop &
AGRES AND SPSS Statistical analysis for biological data.
Submitted the following gene sequences to National Center for Biotechnology
Information (NCBI), USA
Fungi – Inter Transcribed Spacer (ITS) sequence: 18S ribosomal RNA gene, partial sequence;
internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2,
complete sequence; and 28S ribosomal RNA gene, partial sequence (500 to 800 bp lengths)
1. Visalakchi, S., Srinivasan, K. and Muthumary. 2010. Screening of taxol producing endophytic
fungus, Alternaria alternata SVJM015 from medicinal plant, Indigofera enneaphylla
(Accession Number: HM003680).
2. Visalakchi, S., Srinivasan, K. and Muthumary. 2010. Screening of taxol producing endophytic
fungus, Pestalotiopsis oxyanthi SVJM060 from medicinal plant, Taxus baccta (Accession
Number: HM003681).