Phytoplasma diseases in citrus orchards in Pakistan. Shazia Mannan.

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DNA Extraction

DNA was extracted and purified from 1 g of petioles

and midribs using the CTAB method described by

Doyle and Doyle (1990)

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CURRICULAM VITAE - Australasian Plant Pathology Society

DNA Extraction DNA was extracted and purified from 1 g of petioles and midribs using the CTAB method described by Doyle and Doyle (1990)

Sample Analysis Total 20 samples of sweet orange were tested from Sahiwal district: • 15 from farmers’ orchards • 5 from the Horticulture Research Center at Sahiwal.

Page 1 and 2: Shazia Mannan COMSATS Institute of
Page 3 and 4: Following are the main commercial v
Page 5 and 6: • In 1970, Chapot surveyed Pakist
Page 7 and 8: Surveys of citrus Areas N W E S
Page 9 and 10: Sample Collection Citrus Samples Le
Page 11: Insect Sampling Following Insects w
Page 15 and 16: Sequences of Primers Title Sequence
Page 17 and 18: Results 6 samples from farmers’ o
Page 19 and 20: CONCLUSION ‣ Infection with Phyto
Page 21 and 22: Recommendations This project will p
Page 23: ACKNOWLEDGEMENT We would like to ac

Phytoplasma diseases in citrus orchards in Pakistan. Shazia Mannan.

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