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Historical Paper - Volume 2 2008 - International Seed Testing ...

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HISTORICAL REPORT FROM THE PURITY COMMITTEE<br />

In the first ISTA Rules of 1931 it was mentioned that for samples of “any kind of<br />

seed not listed, use a quantity approximating to that given for a similar sized seed”. Since<br />

1976 the issue of ISTA certificates has been restricted to the species mentioned in the<br />

ISTA Rules.<br />

The first ISTA Rules also stated that the results of a purity analysis shall be given<br />

to one decimal place. When the percentage was 75% or less, the results should be given<br />

in whole figures. The reporting of low purity results in whole figures was removed in<br />

1953.<br />

During the ISTA Workshop in Cambridge in 1985 it was discovered that the rounding<br />

off for purity results was performed according to at least three different methods at the<br />

ISTA Laboratories. A working group on calculation of purity results was formed in 1986<br />

and revised Rules for calculation and rounding off purity results was accepted during the<br />

ISTA Congress in 1989.<br />

In 1995 it was accepted that when the mass (weight) of the working sample tested<br />

for purity deviated from that prescribed in table 2A, column 4, the actual mass (weight)<br />

examined shall be reported on the certificate.<br />

Since 1931 the content of other seeds and inert matter and from 1984 the content<br />

of MSU should be reported on the ISTA Certificate if representing 1.0% or more of<br />

the sample. To save time during the purity analysis, since 2001 the occurrence of such<br />

structures should only be reported if requested by the sender of the sample or when<br />

specification is needed according to national or international seed regulations.<br />

The determination of other seeds by number can be performed for one species (e. g.<br />

Avena fatua), a few selected species (e. g. Avena fatua and Cuscuta sp.) or for all other<br />

seeds. Those examinations can be performed in the weight specified in table 2A, column<br />

5 (or in a weight corresponding to 25,000 seeds) or in a reduced amount of seeds. In order<br />

to ensure that the information on the certificate is always unambiguous, the following<br />

endorsements were introduced in the 1976 Rules:<br />

• A Complete test is one in which the whole working sample is searched for all<br />

other seeds present<br />

• A Limited test is one in which the search is restricted to designated species in<br />

the whole working sample<br />

• A Reduced test is one in which only part of the working sample is examined.<br />

In 1998 the following combination was added:<br />

• A Reduced-limited test is one in which less than the prescribed weight of seed<br />

for a working sample is examined for stated species only.<br />

11. Proficiency testing<br />

Since the very beginning of ISTA uniformity in seed testing has been the goal. Prior to<br />

the Congress in Copenhagen in 1921, 25 different seed samples for purity and germination<br />

33

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