Bergey's Manual of Determinative Bacteriology

Identification flow charts
Gram Negative Rods ID Flowchart
Gram Negative Rods
+
Aeromonas, Pseudomonas, Vibrio
Oxidase Test
-
Enterobacteriaceae
(See Separate Flowchartt)
Vibrio spp., Aeromonas spp.
+ Acid - Acid
Glucose Fermentation
Pseudomonas spp.
Na+ Required for Growth
-
Aeromonas spp.
Fluorescent Diffusible Yellow Pigment
(Fluorescein)—use Psuedo F Agar
+
Vibrio spp.
+
V. fischeri
V. logei
V. orientalis
V. splendidius
Luminescent
-
Other Vibrio spp.
(See Bergey's)
+
A. hydrophila
A. salmonicida
(subsp. masoucida)
A. sobria
(no gas from glucose)
A. veronii
VP
Other Aeromonas spp.
(See Bergey's)
Motility
-
+
P. aeruginosa
P. chlororaphis
P. cichorii
P. fluorescens
P. putida
P. syringae
(Oxidase Neg.)
Other Pseudomonas spp.
(See Bergey's)
Non-Fluorescent Diffusible Blue Pigment
(Pyocyanin)—use Psuedo P Agar
-
Pigment
(Yellow Colony)
+
A. hydrophila
A. veronii
-
A. salmonicida
(subsp. masoucida)
-
P. chlororaphis
P. cichorii
P. fluorescens
P. putida
P. syringae
+
P. aeruginosa
+
-
V. fischeri
V. logei
V. orientalis
V. splendidius
H 2 S Production
Nitrate Reduction
+
-
A. hydrophila A. veronii
30°C Growth 37°C Growth
+ +
V. fischeri - V. orientalis -
V. logei
V. splendidius
+
P. chlororaphis
(Colony Pigmented)
P. fluorescens
(To differentiate Biovars
do a lecithinase test)
-
P. putida
(Lecithinase Neg.)
P. syringae
(Oxidase Neg.)
P. cichorii
(Lecithinase Pos.)
6