Label-Free Assays for High-Throughput Monoclonal Antibody ...

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Label-Free Epitope Binning Strategies Used in the Characterization of Fully Human Therapeutic Monoclonal Antibodies Isolated from VelocImmune® Mice Vishal Kamat, Ph.D. Scientist, Therapeutic Proteins Regeneron Pharmaceuticals, Inc. Tarrytown, NY 13 th December, 2012 Confidential VISHAL KAMAT
Presentation Overview ‣ Cross-Competition Assay Formats ‣ Label-Free Technology Platforms ‣ Advantages & Challenges of Different Platforms ‣ Data Analysis ‣ Interpretation of Results Confidential VISHAL KAMAT
Page 1 and 2: Label-Free Assays for High-Throughp
Page 5 and 6: BLI Instruments for Rapid Protein A
Page 7 and 8: Shift (nm) Bio-layer Interferometry
Page 9 and 10: ForteBio Biosensors and Assay Kits
Page 11 and 12: Kinetic Characterization of Protein
Page 13: Label-Free Assays for High-Throughp
Page 17 and 18: Why is Epitope Binning Important?
Page 19 and 20: Different Interaction Analysis Plat
Page 21 and 22: Cross-Competition Assay On Biacore
Page 23 and 24: Cross-Competition Cross-Competition
Page 25 and 26: Schematic of Cross-Competition Assa
Page 27 and 28: Raw Data for a Typical 384 Well Pla
Page 29 and 30: Development of Data Analysis Tool S
Page 31 and 32: Data Analysis - Highlight Self-Self
Page 33 and 34: Data Analysis - Highlight Cells wit
Page 35 and 36: Data Analysis - Move mAb-1 Non-Bind
Page 37 and 38: Data Analysis - Bin mAbs Based on B
Page 39 and 40: Data Analysis - Bin mAbs Based on B
Page 41 and 42: Data Analysis - Representation of C
Page 43 and 44: Tutorial: Drawing Circles to Repres
Page 45 and 46: Challenges: Cross-Competition Data
Page 47 and 48: Biacore 3000/T200 vs Biacore 4000 v
Page 51 and 52: Genmab is a leading international b
Page 53 and 54: Current and future antibody product
Page 55 and 56: State-of-the-Art antibody discovery
Page 57 and 58: Octet RED384 integration to Tecan F
Page 59 and 60: BLI cross-competition designs Sandw
Page 61 and 62: HTS cross-competition process desig
Page 63 and 64: Regeneration of Antibody Biosensor
Page 65 and 66:
Cross-competition binning: HTS BLI
Page 67 and 68:
BLI hybrid library mapping Principl
Page 69 and 70:
Combining Technologies: Biotin Acce
Page 71 and 72:
BLI hybrid library mapping example
Page 73 and 74:
Bead based HTS versus BLI hybrid li
Page 75 and 76:
Correlating degrees of diversity Hy
Page 77 and 78:
BLI cross-competition binning versu
Page 79 and 80:
Summary 79
Page 81:
Acknowledgements Ferdi van der Hors
Page 84 and 85:
Label-Free Assays for High-Throughp
Page 86 and 87:
Page 88:
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Label-Free Assays for High-Throughput Monoclonal Antibody ...

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