Label-Free Assays for High-Throughput Monoclonal Antibody ...

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‣ Regeneron is a fully integrated biopharmaceutical company that discovers, invents, develops, manufactures, and commercializes medicines for the treatment of serious medical conditions – Marketed Products: ARCALYST ® , EYLEA ® & ZALTRAP ® – Product Pipeline: 11 product candidates in various stages of clinical development ‣ In 2012, Regeneron was Rated #1 among all global biopharmaceutical companies by Science magazine ‣ Technology Platforms - VelociGene®, VelociMouse® ‣ VelocImmune® Mice – Produces monoclonal antibodies with human variable region but preserving the mouse constant regions Confidential VISHAL KAMAT
Why is Epitope Binning Important? ‣ Primary Screening: Survey different properties of therapeutic antibodies such as binding affinity, binding specificity & species cross-reactivity, functional properties, etc. ‣ Epitope Binning: Potential to provide additional information to the “Classical” primary screening paradigm ‣ Mode of Receptor Antagonism: Direct vs Steric Crystal Structure of EGFR ectodomain bound to Fab fragments of C225 (Cetuximab) and Matuzumab Fab-C225 Fab-Matuzumab Fab-Matuzumab Confidential Kamat, V. (2010) “Combined Use of Multiple Monoclonal Antibodies Targeting Epidermal Growth Factor Receptor for Improved Cancer Therapeutics”, Ph.D. Drexel University VISHAL KAMAT
Page 1 and 2: Label-Free Assays for High-Throughp
Page 5 and 6: BLI Instruments for Rapid Protein A
Page 7 and 8: Shift (nm) Bio-layer Interferometry
Page 9 and 10: ForteBio Biosensors and Assay Kits
Page 11 and 12: Kinetic Characterization of Protein
Page 15: Presentation Overview ‣ Cross-Com
Page 19 and 20: Different Interaction Analysis Plat
Page 21 and 22: Cross-Competition Assay On Biacore
Page 23 and 24: Cross-Competition Cross-Competition
Page 25 and 26: Schematic of Cross-Competition Assa
Page 27 and 28: Raw Data for a Typical 384 Well Pla
Page 29 and 30: Development of Data Analysis Tool S
Page 31 and 32: Data Analysis - Highlight Self-Self
Page 33 and 34: Data Analysis - Highlight Cells wit
Page 35 and 36: Data Analysis - Move mAb-1 Non-Bind
Page 37 and 38: Data Analysis - Bin mAbs Based on B
Page 39 and 40: Data Analysis - Bin mAbs Based on B
Page 41 and 42: Data Analysis - Representation of C
Page 43 and 44: Tutorial: Drawing Circles to Repres
Page 45 and 46: Challenges: Cross-Competition Data
Page 47 and 48: Biacore 3000/T200 vs Biacore 4000 v
Page 51 and 52: Genmab is a leading international b
Page 53 and 54: Current and future antibody product
Page 55 and 56: State-of-the-Art antibody discovery
Page 57 and 58: Octet RED384 integration to Tecan F
Page 59 and 60: BLI cross-competition designs Sandw
Page 61 and 62: HTS cross-competition process desig
Page 63 and 64: Regeneration of Antibody Biosensor
Page 65 and 66: Cross-competition binning: HTS BLI
Page 67 and 68:
BLI hybrid library mapping Principl
Page 69 and 70:
Combining Technologies: Biotin Acce
Page 71 and 72:
BLI hybrid library mapping example
Page 73 and 74:
Bead based HTS versus BLI hybrid li
Page 75 and 76:
Correlating degrees of diversity Hy
Page 77 and 78:
BLI cross-competition binning versu
Page 79 and 80:
Summary 79
Page 81:
Acknowledgements Ferdi van der Hors
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Label-Free Assays for High-Throughp
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Label-Free Assays for High-Throughput Monoclonal Antibody ...

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