Microsatellite markers

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Tandem repeats (SSR; Simple sequence repeat): Sequences that are present in multiple copies adjacent to one another. SSLP (Simple sequence length polymorphism) e.g., satellite, minisatellite (VNTR; variable number TR), microsatellite (STR; short tandem repeat) All belong to SSR but with different repeat length.
= VNTR = variable numbers of tandem repeats Consists of runs of dinucleotide repeats, most commonly CA(or TG on complementary strand), that are dispersed throughout the genome.
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Melting curve & DNA length
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SYBR Green I stain is a highly sens
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254nm
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Real-Time PCR with SYBR Green
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Real-Time PCR with SYBR Green
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Microsatellite markers

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