CURRICULUM VITAE Cleo Adelle Hughes-Darden, Ph.D ...

CURRICULUM VITAE
Cleo Adelle Hughes-Darden, Ph.D.
CURRENT ADDRESS
Morgan State University Office: Richard Dixon 213
Department of Biology Lab: Richard Dixon 214
1700 East Cold Spring Lane Phone: (443) 885-3635 (Office)
Baltimore, MD. 21251
Phone: (443) 885-4466 (Lab)
Email: chughes@moac.morgan.edu FAX: (443) 885-8285
EDUCATION
1991 Clark Atlanta University, Atlanta, GA. Ph.D. Microbial Physiology and Molecular Biology
1986 Morgan State University, Baltimore, MD. B.S. Biology
RESEARCH AND PROFESSIONAL EXPERIENCE
2002- Associate Professor, Morgan State University, Department of Biology, Baltimore,
Maryland
8/93 - 2002 Assistant Professor, Morgan State University, Department of Biology, Baltimore,
Maryland
1991- 1993 Post-doctoral Research Associate, USDA/ARS, Plant Molecular Biology
Laboratory, Beltsville, MD.
1986 - 1991 Ph.D. Biology, Clark Atlanta University, Atlanta, GA
1986 - 1991 NIH National Research Service Award (Predoctoral Trainee), Clark Atlanta
University, Atlanta, GA.
1989- 1990 Student Representative for NRSA Policy and Admissions Committee, Clark
Atlanta University, Atlanta, GA.
9/89- 12/89 Graduate Teaching Assistant, Clark Atlanta University, Atlanta, GA
9/88- 12/88 Graduate Teaching Assistant, Morehouse College, Atlanta, GA.
6/86-8/86 Laboratory Technician, Department of Pediatrics, University of Maryland,
Baltimore, MD.
1982 - 1986 B.S. Biology, Morgan State University, Baltimore, Maryland
6/85-8/85 Research Assistant, Department of Pediatrics, University of Maryland, Baltimore,
Maryland
1984-1986 NIH Minority Access to Research Careers Honors Undergraduate Research Trainee,
Morgan State University, Baltimore, Maryland
HONORS AND AWARDS
Recipient of travel award to attend 3 rd International Conference on Brain Energy Metabolism, 1997
Whos Who Among Students in Colleges and Universities, 1990-1991
Co-Recipient of Nabrit Symposium Outstanding Poster Award, 1989
Co-Recipient of Frederick E. Mapp Outstanding Poster Award, 1989
Recipient of NIH National Research Service Award, Clark Atlanta University, 1986-1991
Recipient of Excellence in Scientific Research Award, Beta Kappa Chi Convention , 1986

MEMBERSHIP IN PROFESSIONAL SOCIETIES
American Society for Biochemistry and Molecular Biology 1999
American Society for Cell Biologists
1997-present
American Society of Plant Physiologists 1992-2001
International Society for Plant Molecular Biology 1992-2001
American Society for the Advancement of Science
1990-present
American Society for Microbiology 1988-1992
PROFESSIONAL TRAINING
Selected as a participant in the 2000 Current Topics in Genetic Research Short Course by the National
Human Genome Research Institute (NHGRI) at the National Institutes of Health on August 7-11, 2000
Selected as a participant in the Quality Education for Minorities (QEM) network which will held at North
Carolina Central University in Durham North Carolina November 12-13, 1999.
Selected as a participant in the Grant writing workshop at the University Kentucky on August 16-17, 1999.
Attended MORE Program Directors Meeting, Chantilly, VA, June 20-23, 1999
Grantsmanship Workshop, Morgan State University, Baltimore, Maryland, May 20-21, 1999
Shaping the Future: Teaching Science to Undergraduates in the 21 st Century Workshop, Atlanta, GA.,
October, 1998
Attended Conference on PKC and Protein phosphorylation, Lake Tahoe, CA, October, 1998
In situ Hybridization Workshop, Gaithersberg, MD., September, 1998
Grantsmanship Workshops held at Morgan State University sponsored by the RIMI program: May 20-21
1997 and May 26-27 1998.
Recombinant DNA Methodology and Special Topics in Biotechnology Workshop, Bethsda, MD., January,
1995
PROFESSIONAL ACTIVITIES
Reviewer for NIH Bridges Program 3/2000 and 11/2000
Reviewer for National Science Foundation 9/99
Reviewer for National Defense Science and Engineering Graduate Fellowship program, 2/99
Reviewer for manuscripts submitted to Cell and Molecular Biology journal 1996- present
Co-Chair of Symposium XII Cell Signaling at 2 nd World Congress Cellular and Molecular Biology,
Ottawa, Canada, 1996
Visiting Scientist at USDA/ARS, Plant Molecular Biology Laboratory, 1993-1998
GRANTS FUNDED
PI: Cleo A. Hughes Source: NSF #IBN09602190
Title: Characterization of Cystathionine gamma-synthase in soybean Dates: 8-1-96 to 7-31-98
PI: T.J. Robinson Co-PI: Cleo A. Hughes Source: NIH/NIGMS-
1S06GM/51971-01
Title: Gamma-2-MSH receptors, cellular signaling, calcium and hypertension Dates: 2-1-96 to 1-31-99
PI: T. J. Robinson Co-PIs: Cleo A. Hughes and Michael Koban Source: NIH/NCRR#2 P20
RR11606-02
Title: Stress and Cardiovascular Diseases Dates: 10-1-96 to 9-30-02

PI: Cleo Hughes-Darden Co-PI: James Wachira Source: NIH/NCRR# G12RR17581-02
Title: Neuromelanocortin system and gene expression during stress Dates: 10-1-02 to 9-30-07
PI: James Wa chira Co-PI: Cleo Hughes-Darden Source: NIH/NIGMS# 2SO6-GM051971-08
Title: Melanocortin Signaling In Stroke-Prone Hypertensive Rats Dates: 3/1/03-2-28-07
RESEARCH ACTIVITIES AND INTERESTS
Presently, my lab is interested in understanding the role that the components of the phosphoinositide
signaling system and other signaling pathways play in the stress response and the effects on the
cardiovascular system. We are using two model systems (a genetic model for hypertension (SHR) and a
stress induced model, stress induced by sleep deprivation). Also, we are interested in determining the role
of the POMC peptides (γ-2-MSH and ACTH) on the expression of protein kinases such as PKC, MAP
kinases and tyrosine kinases and on phosphorylation of specific substrate proteins in these model systems.
Another aspect of our research, is to examine the expression and regulation of genes that are expressed in
response to stressors such as sleep deprivation stress and hypertension using microarray technology and to
fully characterize these genes.
SEMINARS :
1. Hughes, C.A. Does gamma-2MSH activate protein kinase C (PKC) in synaptosomes isolated
from hypertensive rats? RIMI Symposium 2/2000
2. Hughes, C.A. Genetic engineering of plant amino acid biosynthetic pathways. Morgan State
University RIMI seminar series (1999).
3. Hughes, C.A. Characterization of Cystathionine gamma synthase in soybean. Spelman College
RIMI seminar series (1999).
4. Hughes, C.A., Stewart, C.V. , Koban, M. and Robinson, T.J. Effects of stress induced by sleep
deprivation on protein kinase C (PKC) isozymes expression in rat synaptosomes. The 1999
Annual NIH-RCMI Program Directors= Meeting and Scientific Conference on Ethinicity and
Aging in the 21 st Century (1999)
5. Hughes, C.A., Adams, Twyla D., Jenkins, Marshell, Robinson, T. Joan and Ochillo, Richard F.
Differential expression of the protein kinase C (PKC) family of isozymes in synaptosomes from
brains of rats stressed by sleep deprivation. Cell and Molecular Biology 42: 40 (1996).
6. Hughes, Cleo A. and Benjamin F. Matthews Molecular cloning and expression of asparagine
synthetase from soybean. J. of Cellular Biochemistry 18:105 (1994).
TEACHING EXPERIENCE
Undergraduate level
General Biology, Biology 101
General Biology, Biology 102
Cell and Molecular Biology, Biology 310
Scientific Communication, Biology 453
Concepts in Computational Biology, Biology 454

Graduate level
Cell Biology, Biology 525
Molecular Biology, Biology 526
STUDENT TRAINING
Graduate students
Craig Stewart BRIDGES (1997-2001)
Shelley Wortham BRIDGES (1997-2000)
Undergraduate students
Timothy Craddock MARC U*STAR (1993-1996)
Twyla Adams MARCU*STAR (1995-1996)
Christopher Taylor MBRS-RISE (1995-1996)
Kiana Brunson MBRS-RISE (1996-1999)
Malene Robinson MARC U*STAR (1996-1998)
Monaliza Tewelde NSF (1996-1997)
Eric Spencer NSF (Fall 1997)
Zari Anthony NSF(Spring 1998)
Esther Batta NSF(Summer 1998)
Ryan Springer MARC U*STAR (Summer 1998-Spring 2000)
Angela Reves
MBRS (Summer 1998- Spring1999)
Collins Karikari BRIDGES (Summer 1998)
Zari Anthony MARC U*STAR (Summer 1999-Spring 2000)
Kathlene Babaloba SEM student Summer 2000; (Spring 2000- Spring 2001)
Miriam Isles MARC U*STAR Student (Summer 2000- Spring 2002)
Ugochi, Ekeocha MARC U*STAR (Summer 2000- Spring 2002)
Casia, Thompson MBRS-RISE (Summer 2001- Spring 2002)
John Cargo III
MBRS-RISE (Summer 2003-present)
High school
Howard Calhoun NASA SHARP PLUS (Summer 1997)
Antonio Baca NASA SHARP PLUS (Summer 1998)
COMMITTEES
Departmental Committees
Senior Comprehensive 1997-2002
Search 2002-present
Biology Department Alumni 1993-present
Promotion and Tenure 2002- present
Research Committee 1995-2002
Library Committee 1995-2002
Curriculum Committee 1997-2002
Graduate Program Committee 1997-2002
School Committees
Research symposium
Marshal for graduation 1995-1998

University Committees
Co-director of MARC program
MARC program Advisory committee
Marshal for Graduation
BIBLIOGRAPHY
GenBank Accession Numbers
77678 Glycine max asparagine synthetase 2 (1997)
77679 Glycine max asparagine synthetase 1 (1997)
AF141602 Glycine max cystathionine gamma-synthase complete cDNA sequence (1999)
Published Journal Articles:
1. .Wachira, J.M, Hughes-Darden, C.A., Nicholas, H.B., Taylor, C.V., and T. Joan Robinson
(2004) neural Melanocortin Receptors are Differentially Expressed and Regulated by stress in rat
hypothalamic-pituitary-adrenal axis. Cellular and Molecular Biology Journal 50:703-713.
2. Wachira, J.M, Hughes-Darden, C.A., Taylor, C.V., Ochilli, R. and T. Joan Robinson (2003)
Melanocortin 3- Receptor Signaling in Neuronal Cells involves the Protein Kinase C Pathway
Neuropeptides 37:201-210.
3. Hughes-Darden, C.A., Wachira, S. James, Denaro, Frank J., Taylor, Christopher V., Brunson, Kiana
J., and Robinson, T.J. (2001) Expression and Distribution of protein kinase C isozymes in brain tissue of
Spontaneous Hypertensive rats (SHR). Cellular and Molecular Biology.
47:1077-1088.
4. Hughes-Darden, C.A., Wachira, S. James, Batta, Esther, and Robinson, T. Joan (2001) Effects of
gamma-2-melanocyte-stimulating hormone on protein kinase c activity and expression in spontaneous
hypertensive rats (SHR). Cellular and Molecular Biology 47:1069-1075.
5. Hughes, C. A., Gebhardt, J. S., Reuss, A., and Matthews, B.F.
(1999) Identification and Expression of a cDNA encoding cystathionine-γ-synthase in soybean. Plant
Science 146:69-79.
6. Hughes, Cleo A., Beard, Hunter and Matthews, Benjamin F. (1997) Molecular cloning and
expression of two cDNAs encoding asparagine synthetase in soybean Plant Molecular Biology 33:301-
311.
7. Matthews, Benjamin F. and Hughes, Cleo A. (1993) Nutritional improvement of the family of
amino acids in edible crop plants . Amino Acids 4:21-34.
8. Hughes, Cleo A. and Benjamin F. Matthews (1992) Cloning a cDNA encoding asparagine
synthetase from soybean. p. 345-346. In: BK Singh, HE Flores, JC Shannon (eds), Biosynthesis and
Molecular Regulation of Amino Acids in Plants. Proceedings of the Seventh Annual Penn State
Symposium in Plant Physiology.

Manuscripts in Preparation
Cargo, J.C., Redmond, B., Wachira, S.J., and Hughes-Darden, C.A. (2005) Role of PKC-alpha
during stress induced by sleep deprivation in rat ventricular membranes.(in preparation).
Hughes-Darden, C.A., Wachira, S.J., and Redmond, B. (2005) Microarray analysis of gamma-
2- melanocyte-stimulating hormone (MSH) responsive genes in CAD neuronal cell line.
Abstracts
Hughes-Darden, C.A., Wachira , James, and Taylor, Christopher. Analysis of Gene Expression in Rat Brain
Following REM-Sleep Deprivation Stress. RCMI Internal Research Symposium: Morgan State Univ. 0ral
(2004)
Wachira, James, Hughes-Darden, Cleo, Prioleau, Algernon and Robinson, T. Joan. Melanocortin 3
receptor signaling in neuronal cells. FASEB Journal 69.19. (2004).
***John Cargo III, Brian Redmond, Duewa Williams, James Wachira, and Cleo Hughes-Darden
Differential Expression of Cardiac Protein Kinase C Isozymes in Rats after Sleep Deprivation (SD) Stress.
RCMI Symposium 2004.
James M Wachira, Cleo Hughes-Darden and Algernon Prioleau. Identification of Biomarkers for
Hypertension via a Proteomic Approach. RCMI Symposium (2004).
James Wachira, Lawrence Uladu, Carlana Ramlochansingh, Bindhu Guruswamy and Cleo Hughes-
Darden Alteration Of Neural Melanocortin System Gene Expression In Stroke Prone SHR Rats By High
Salt Diet.RCMI Symposium (2004).
Lawrence Uradu, Cleo Hughes-Darden and James Wachira. Characterization of Melanocortin 3 Receptor
Signaling Pathway in a Brain Stem Neuronal Cell Line. RCMI Symposium (2004).
Hughes-Darden, C.A., Wachira, J.M., Taylor, C. and Robinson, T.J. The involvement of PKC
isozymes in neural melanocortin signaling. FASEB annual meeting, San Diego, CA. April 2003.
(Competitively selected for an oral presentation and poster).
Wachira, J.M., Hughes-Darden, C.A., Taylor, C.V and Robinson, T.J. Protein SER/THR kinases in
neural melanocortin signaling. 2 nd Biennial RIMI Research sysposium p. 39 (oral) (2002).
Taylor, C.V., Wachira, J.M., Hughes-Darden, C.A. and Robinson, T.J. Evidence for the involvement of
protein kinase C in melanocortin 3-receptor signaling. 2 nd Biennial RIMI Research sysposium CV06: p. 39
(2002).
Batta, E., Hughes-Darden, C.A., Ochilli, R., Robinson, T.J. Role of protein kinase C isozymes in neural
melanocortin signaling. 2 nd Biennial RIMI Research sysposium CV08: p.40 (2002).
Stewart, C.V., Batta, E., Wachira, J.M., Robinson, T.J., Hughes-Darden, C.A. The effects of gamma-2-
MSH on PKC activity in brain synaptosomes from rapid eye movement (REM) sleep deprived rats 2 nd
Biennial RIMI Research sysposium CV07:p.40 (2002).

Isles, M., Stewart, C.V., Wachira, J., Robinson, T.J. and Hughes-Darden, C.A.,
Role of renal protein kinase C (PKC) in rapid eye movement sleep deprivation (REM-SD) stress.
2 nd Biennial RIMI Research sysposium CV02:p44 (2002).
Ekeocha, U., Stewart, C.V., Wachira, J., Robinson, T.J. and Hughes-Darden, C.A.
Increases in cardiac protein kinase C (PKC) following rapid eye movement sleep deprivation (REM-SD)
stress. 2 nd Biennial RIMI Research sysposium CV01:p45 (2002).
Ekeocha, U., Stewart, C., Wachira, J.M, Robinson, T.J. and Hughes-Darden, C.A, Rapid eye movement
sleep deprivation (REM-SD) stress modulates expression of crdiac protein kinase C (PKC). 9 th Annual
Undergraduate and Graduate Research Symposium B-3:p.5 (2002).
Isles, M., Stewart, C.V., Wachira, J., Robinson, T.J. and Hughes-Darden, C.A. Effects of rapid eye
movement sleep deprivation (REM-SD) on renal protein kinase C (PKC). 9 th Annual Undergraduate and
Graduate Research Symposium B-4:p. 5 (2002).
Wachira, J.M., Hughes-Darden, C.A., Taylor, C.V., Robinson. T.J. Role of Ser/Thr Protein Kinases in
Melanocortin 3-Receptor Signaling. (Submitted to FASEB J.) (2002)
Isles, M. Stewart, C., Hughes-Darden, C.A, Wachira, J.M, Robinson, T.J. Effects of sleep deprivation
stress on protein kinase C in rat kidney. Mol. Biol. of the Cell 12:405a,2229 (2001).
Ekeocha, U., Stewart, C., Hughes-Darden, C.A, Wachira, J.M, Robinson, T.J. Elevation of PKC activity
in rat heart following rapid eye movement sleep deprivation stress. Mol. Biol. of the Cell 12:405a,2230
(2001).
Ekeocha, U., Stewart, C.V., Hughes-Darden, C.A., Wachira, J., Robinson, T.J. Rapid eye movement
sleep deprivation stress induces elevation of PKC activity in the rat heart.
8 th Annual Undergraduate and Graduate Research Symposium B-12:p.9 (2001).
Hughes, C.A., Wachira, J.M., Stewart, C.V., and Robinson, T.J. Analysis of gene expression in rat brain
following REM-Sleep deprivation. FASEB J. 15:A483:414.5 (2001).
Wachira, J.M., Hughes, C.A., Stewart, C.V., Batta, E.N., and Robinson, T.J. Activation of protein kinase
C (PKC) by gamma-2-MSH in rat brain synaptosomes. FASEB J. 15:A176:179.1 (2000).
Springer, R., Stewart, C.V., Hughes, C.A. and T.J. Robinson. (2000) The expression of PKC isozymes in
rats stressed by sleep deprivation. 57 th Annual meeting Beta Kappa Chi, National Institute of Science
Brookhaven Semester Program p. 33 (2000).
C.A. Hughes, J.M. Wachira, G.R. Desir, and T.J. Robinson. Does gamma-2-MSH activate protein kinase
C (PKC) in synaptosomes isolated from hypertensive rats? RIMI Symposium. p.O-07. Oral presentation
(2000).
J.M. Wachira, C.A. Hughes, and T.J. Robinson. Effects of gamma-2-MSH on intracellular calcium levels
in rat brain synaptosomes. RIMI Symposium p. O-03 (2000).

C.V. Stewart, C.A. Hughes, J.M. Wachira, M. Koban, and T.J. Robinson. Gamma-2-melanocyte
stimulating hormone effects on protein kinase C activation in brain synaptosomes from sleep deprived rats.
RIMI Symposium p. O-09 (2000).
S.S. Wortham, C.A. Hughes, J.M. Wachira, and T.J. Robinson. The effects of REM sleep deprivation on
protein tyrosine kinase activity in rat brain. RIMI Symposium R-10 (2000).
S.S. Wortham, C.A. Hughes, M. Koban, T.J. Robinson. The effects of stress by REM sleep deprivation
on total activity of protein tyrosine kinases in rat brain synaptosomes. Molecular Biology of the Cell
10:53a,308 (1999).
C.V. Stewart, C.A. Hughes, M. Koban and T. J. Robinson. The effects of gamma-2-MSH on PKC
activation and translocation in brain synaptosomes from sleep deprived rats. Molecular Biology of the
Cell 10:333a:1926 (1999).
Springer, R.R., Stewart, C.V., Hughes, C.A., T.J. Robinson. The expression of PKC isozymes in heart
tissue isolated from rats stressed by sleep deprivation. 6 th Annual Undergraduate and Graduate Science
Research Symposium p. 5. (1999)
S. Wortham, C.A. Hughes, T.J. Robinson. The effects of stress by REM sleep deprivation on levels of
expression and activity of protein tyrosine kinase in rat brain. 6 th Annual Undergraduate and Graduate
Science Research Symposium. P.6 (1999).
B. Manswell, C.A. Hughes, M. Koban, and T.J. Robinson. The effect of stress induced by rapid eye
movement sleep deprivation on the expression and activity of mitogen activated protein (MAP kinases). 6
th Annual Undergraduate and Graduate Science Research Symposium p. 10 (1999).
Hughes, C.A., Stewart, C.V. , Koban, M. and Robinson, T.J. Effects of stress induced by sleep
deprivation on protein kinase C (PKC) isozymes expression in rat synaptosomes. Annual NIH-RCMI
Program Directors= Meeting and Scientific Conference on Ethinicity and Aging in the 21 st Century (1999)
Stewart, C.V., Taylor, C.V., Hughes, C. A., Koban, M. and Robinson, T.J. Expression of protein kinase
C (PKC) isozymes in synaptosomes isolated from rats stressed by sleep deprivation. Molecular Biology of
the Cell 9:482a-2794 (1998).
Manswell, B., Taylor, C.V., Hughes, C.A., Koban, M. and Robinson, T.J. The effect of stress induced by
sleep deprivation on the expression of MAP kinases. Molecular Biology of the Cell 9:231a-1339 (1998).
Koban, M., Jones, S., Hughes, C.A., and Robinson, T. J. Sleep deprivation stress induces tissue-specific
expression of heat-shock proteins. Molecular Biology of the Cell 9:317a-1837 (1998).
Hughes, Cleo A., Gebhardt, Joan S., Reuss, Andree, MacDonald, M., Samuels, Andrea, and Matthews,
Benjamin F. Cloning and expression of a soybean cDNA encoding cystathionine-γ-synthase. In vitro
Cellular and Developmental Biology P-1084:63A (1998).
Robinson, T.Joan and Hughes, C.A. The effects of gamma-2-MSH on DAG activity in synaptosomes
from hypertensive and normotensive rats. In vitro Cellular and Developmental Biology V-1032:33A
(1998).

Hughes, C.A., Taylor, C.V., Jenkins, M.C., Animashaun, L., Stewart, C.V., Koban, M. and Robinson, T.J.
Diacylglycerol activation of PKC δ and PKC ε in synaptosomes from normal and hypertensive rats.
Molecular Biology of the Cell 8:68 (1997).
Hughes, C.A., Taylor, C.V., Stewart, C.V., Brunson, K., Carmichael, D. and Robinson, T.J. Does
hypertension affect the levels of PKC isozymes in the brain?
3 rd International Conference on Brain Energy Metabolism #42 (1997).
Hughes, C. A., Gebhardt, Joan S., Reuss, Andree, Samuels, Andrea, and Matthews, Benjamin F. Cloning
of cDNA encoding cystathionine gamma- synthase in soybean. Keystone symposium on Molecular and
Cellular Biology p. 24 (1997).
Taylor, C.V., Brunson, K., Hughes, C.A., Stewart, C.V., Carmichael, D. and Robinson, T.J. The effect of
γ-2-MSH on the expression of protein kinase C isozymes from brain synaptosomes of SHR and WKY rats.
FASEB Journal A138 (1997).
Stewart, C.V., Carmichael, D., Jenkins, M., Taylor, C.V., Brunson, K., Hughes, C.A.,
and Robinson, T.J. Proopiomelanocortin (POMC) derived peptides immunoreactivity in brain regions of
normal and hypertensive rats. FASEB Journal A107 (1997).
Taylor, Christopher V., Brunson, Kiana, Carmichael, Dana K., Stewart, Craig V.,
Jenkins, Marshell, Hughes, C. A., and Robinson, T. Joan. Expression of protein kinase C (PKC) Isozymes
in synaptosomes from spontaneous hypertensive (SHR) rats. Annual MBRS/MARC meeting B28, p.31
(1996).
Carmichael, Dana K., Stewart, Craig V., Jenkins, M., Taylor, Christopher V., Brunson, K. and Hughes,
C.A., and Robinson, T.Joan PKC isozyme immunoreactivity in brain regions from normal and
hypertensive rats. Annual MBRS/MARC meeting B30, p.31 (1996).
Hughes, C.A., Adams, Twyla D., Jenkins, Marshell, Robinson, T. Joan and Ochillo, Richard F.
Differential expression of the protein kinase C (PKC) family of isozymes in synaptosomes from brains of
rats stressed by sleep deprivation. Cell and Molecular Biology 42: 40 (1996).
Robinson, T.J., Ochillo, R.F., Smith, M., Tsai, C., Hughes, C.A., and Olubadewo, J. The effect of
different agonist and antagonist on the activites of synaptosomal protein kinase A (PKA) and protein
kinase C (PKC) from regions of the brain of animals stressed by sleep deprivation. FASEB Journal
10:A606 (1996).
Greene, S., Robinson, T.J., and Hughes, C.A. The effect of gamma-2-melanocyte stimulating hormone (γ-
2-MSH) on the activation of protein kinase C (PKC) in brain synaptosomes from normal and hypertensive
rats. FASEB Journal 10:A606 (1996).
Hughes, Cleo A., Craddock, Timothy L. and Matthews, Benjamin F.
Molecular cloning and characterization of two soybean cDNA clones encoding asparagine synthetase.
Engineering plants for commercial products and applications
p. 21 (1995).

Hughes, Cleo A. and Benjamin F. Matthews Molecular cloning and expression of asparagine synthetase
from soybean. J. of Cellular Biochemistry 18:105 (1994).
Hughes, Cleo A. and Benjamin F. Matthews Expression of a soybean asparagine synthetase gene in
Escherichia coli K-12. Plant Physiology 102:18 (1993).
Matthews, Benjamin F., Gebhardt, Joan S., Hughes, Cleo A., Silk, Gregg W., and Wadsworth Gregory J.
Cloning and expression of genes encoding enzymes involved in the synthesis of the aspartate family in
soybean. Amino Acids 5:183-184 (1993).
Matthews, Benjamin F., Gebhardt, Joan S., Hughes, Cleo A., Silk, Gregg W., and Wadsworth, Gregory J.
Molecular characterization of amino acid biosynthesis in soybean. Seventeenth International Congress of
Genetics p. 71 (1993).
Hughes, Cleo A. and Williams, Arthur L. Molecular analysis of leucine-sensitivity in Escherichia coli K-
12. ASM SEB p. 22 (1990).
Hughes, Cleo A. and Williams, Arthur L. Characterization of leu R and ilvA + DNA fragments of
Escherichia coli K-12. ASM H61 (1989).
Hughes, Cleo A. and Williams, Arthur L. Genetic analysis of a leucine-sensitive conferring mutation of
Escherichia coli K-12. ASM SEB p. 51(1988).
Hughes, Cleo A. and Williams, Arthur L. Genetic analysis of recombinant plasmids carrying ilvA + and
leu R DNA fragments of Escherichia coli in ilvA mutant strains ASM H73 (1988).