Urea Nitrogen (BUN) - temaricerca.com

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WEB INSERT 2011-02-04 As s ay Principle The DetectX® Urea Nitrogen (also called BUN) Detection Kit is designed to quantitatively measure urea nitrogen in a variety of samples. Please read the complete kit insert before performing this assay. A urea nitrogen standard calibrated to NIST reference materials is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with Color Reagents A and B and incubated at room temperature for 30 minutes. The colored product is read at 450 nm. The concentration of urea nitrogen in the sample is calculated, after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of mg/dL urea nitrogen. If samples are to be expressed in terms of mg/dL urea, the data can be converted using the multiplier 2.14. DetectX ® Kits Re l at e d Pr o d u c t s Serum Creatinine Detection Kits Catalog Number KB02-H1 /H2 Retinol Binding Protein Urinary EIA Kit Cystatin C EIA Kit Hemoglobin Dual Range Detection Kit Catalog Number KU04-H1 Catalog Number K012-H1 Catalog Number K013-H1 Su p p l i e d Co m p o n e n t s Clear 96 well Plates 2 Plates Catalog Number X003-2EA Urea Nitrogen Standard 250 µL Catalog Number C089-250UL Urea Nitrogen at 100 mg/dL in a special stabilizing solution. Calibrated to NIST Standard Reference Material Lot Number 912a Color Reagent A 15 mL Catalog Number X094-15ML An acidic solution of Color Reagent A. CAUTION: CAUSTIC Color Reagent B 15 mL Catalog Number X095-15ML An acidic solution of Color Reagent B. CAUTION: CAUSTIC St o r a g e In s t r u c t i o n s All components of this kit should be stored at room temperature until the expiration date of the kit. ® 4 www.ArborAssays.com
Distilled or deionized water free of urea. WEB INSERT 2011-02-04 Ot h e r Mat e r i a ls Re q u i r e d 96 well plate reader capable of reading optical absorption at 450 nm. Software for converting optical density (OD) readings from the plate reader and carrying out four parameter logistic curve (4PLC) fitting. Contact your plate reader manufacturer for details. Pr e c a u t i o n s As with all such products, this kit should only be used by qualified personnel who have had laboratory safety instruction. The complete insert should be read and understood before attempting to use the product. The Color Reagents A and B are both strong acid solutions and should be handled like any laboratory acid. Sa m p l e Ty p e s Urea nitrogen is identical across all species and this kit will measure urea nitrogen from sources other than human. The end user should evaluate recoveries of urea nitrogen in samples from other species being tested. The kit will measure urea nitrogen in low concentration samples such as cell culture media, however the appropriate dilutions will have to be determined by the end user. If samples need to be stored after collection, we recommend storing them at -70°C or lower, preferably after being frozen in liquid nitrogen. This assay has been validated for serum, plasma and urine. Samples containing visible particulate should be centrifuged prior to using. Sa m p l e Pr e pa r at i o n Dilute sample with distilled or deionized water prior to running in the assay. For serum or plasma, the recommended dilution is ≥ 1:10 and ≥1:20 respectively. For urine, where concentrations of urea are higher, the recommended final dilution is ≥ 1:100. For highly colored samples, dilution greater than 1:10 or 1:100 may be necessary. ® www.ArborAssays.com 5
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