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Urea Nitrogen (BUN) - temaricerca.com

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Typical Standard Curve<br />

WEB INSERT 2011-02-04<br />

2.0<br />

1.8<br />

1.6<br />

1.4<br />

Net OD<br />

1.2<br />

1.0<br />

Net OD<br />

0.8<br />

0.6<br />

0.4<br />

0.2<br />

0.0<br />

0 2 4 6 8 10<br />

<strong>Urea</strong> <strong>Nitrogen</strong> Conc. (mg/dL)<br />

Always run your own standard curves for calculation of results.<br />

Do not use these data.<br />

Validation Data<br />

Sensitivity and Limit of Detection<br />

Sensitivity was calculated by <strong>com</strong>paring the ODs for twenty wells run for each of the zero and<br />

standard #7. The detection limit was determined at two (2) standard deviations from the zero<br />

along the standard curve.<br />

Sensitivity was determined as 0.030 mg/dL.<br />

The Limit of Detection was determined in a similar manner by <strong>com</strong>paring the ODs for twenty wells<br />

run for each of the zero and a low concentration human sample.<br />

The Limit of Detection was determined as 0.065 mg/dL.<br />

®<br />

8<br />

www.ArborAssays.<strong>com</strong>

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