Zimmermann Hamdani, Wolfgang A. Linke, Xiaoke Yin, Manuel ...

#CIRCRESAHA/2011/251843/R1
Online Figure VII. Development of a cardiomyocyte-specific qPCR assay to allow
approximation of cardiomyocyte content in mixed cell populations. Detection of CSQ2 (NCBI
accession#: AF001334; squares) and GAPDH (NCBI accession#: NC005103; circles) transcripts in
2.5x10 6 cells containing the indicated cardiomyocyte fraction by qPCR. High Ct-values indicate low
transcript abundance. Symbols indicate individual Ct-values. Bars indicate the respective means. A
Ct-value difference of 3.3 ideally represents a 10-fold difference in transcript abundance. Similar data
could be obtained using alternative primer-probe pairs for muscle specific CSQ2 (Ensemble
accession#: ENSRNOG00000016243) or 18S-RNA (NCBI accession#: V01270).
Online Video I: Spontaneous contractions in EHTs on culture day 3.
Online Video II: Spontaneous contractions of EHTs on culture day 7.
Online Video III: Spontaneous contractions of EHTs on culture day 12.
Online Video IV: Animated 3D reconstruction of adjacent optical sections from EHTs on
culture days 0, 3, 7, and 12.
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