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Zimmermann Hamdani, Wolfgang A. Linke, Xiaoke Yin, Manuel ...

Zimmermann Hamdani, Wolfgang A. Linke, Xiaoke Yin, Manuel ...

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Online Figure II<br />

A<br />

Day 0<br />

B<br />

2N 4N 8 N<br />

2N 4N 8N<br />

100<br />

Alpha-actinin fluorescence<br />

Day 12<br />

2N 4N 8N<br />

% of cardiomyocytes<br />

% of non-myocytes<br />

80<br />

60<br />

40<br />

20<br />

0<br />

100<br />

80<br />

60<br />

40<br />

20<br />

Day 0 Day 12<br />

0<br />

DNA content<br />

Day 0 Day 12<br />

Online Figure II. DNA content in mononucleated cardiomyocytes and<br />

fibroblasts during EHT culture assessed by FACS. A, Representative plots of<br />

DNA content in mononucleated cardiomyocytes (actinin-positive, red) and nonmyocytes<br />

(actinin-negative, dark grey) at day 0 and day 12 of EHT culture. B,<br />

Fractions of mononucleated cardiomyocyte (top panel) and non-myocyte (lower<br />

panel) populations at day 0 (n=6) and day 12 (n=4) of EHT culture with 2N, 4N, and<br />

8N DNA content.<br />

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