SRID vs. EIA For Quantitation of Influenza Hemagglutinin - GTCbio
SRID vs. EIA For Quantitation of Influenza Hemagglutinin - GTCbio
SRID vs. EIA For Quantitation of Influenza Hemagglutinin - GTCbio
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<strong>SRID</strong> <strong>vs</strong>. <strong>EIA</strong><br />
<strong>For</strong> <strong>Quantitation</strong> <strong>of</strong> <strong>Influenza</strong> <strong>Hemagglutinin</strong><br />
Steve Rockman PhD. Msc. B. App.SC.<br />
Manager, <strong>Influenza</strong> Innovation<br />
CSL Ltd<br />
GTC, a conference production company • (626) 256-6405 • www.gtcbio.com
<strong>SRID</strong> <strong>vs</strong>. <strong>EIA</strong><br />
<strong>For</strong> <strong>Quantitation</strong> <strong>of</strong> <strong>Influenza</strong> <strong>Hemagglutinin</strong><br />
Steve Rockman PhD. Msc. B. App.SC.<br />
July 2012
Delay between pandemic declaration and<br />
availability <strong>of</strong> Pandemic vaccine.<br />
Ability to quantitate antigen - paramount.<br />
?
3<br />
6/8/2012<br />
Summary <strong>of</strong> presentation<br />
• New capture detection method for quantitation<br />
• Correlation with <strong>SRID</strong><br />
• Stability indicating<br />
• Timelines<br />
• Extended life <strong>of</strong> reagents<br />
• NA quantitation - progress
<strong>Quantitation</strong> <strong>of</strong> vaccine antigen (HA)-<br />
Single radial Immunodffusion<br />
• Antibody in a 1% agarose gel<br />
with detergent<br />
• Antigen loaded into well<br />
• Detects immuno-relevant<br />
material
Single radial Immunodffusion – approved<br />
method<br />
•Time consuming<br />
•~20% coefficient variation<br />
• Low sensitivity<br />
• Lab to Lab variation<br />
• Unable to be automated<br />
• frequent delays for reagents<br />
(antigen and antiserum)<br />
A/Indonesia/5/05 double ring
6<br />
Detects<br />
immunorelevant<br />
material<br />
6/8/2012<br />
Alternative HA assays<br />
<strong>SRID</strong> <strong>EIA</strong><br />
(capture/detect)<br />
Biosensor HPLC SDS-PAGE Mass<br />
spectroscopy<br />
✓ ✓ ✓ ✗ ✗ ✗<br />
Stability<br />
indicating ✓ ✓ ✓ ✗ ✗ ✗<br />
Delay <strong>of</strong><br />
reagents ✗ ✓ ✓ - - -<br />
Extended life <strong>of</strong><br />
reagents ✗ ✓ ✓ - - ✓<br />
Able to be<br />
automated ✗ ✓ ✓ ~ ✗ ~<br />
High Sensitivity<br />
✗ ✓ ✓ ✓ ✗ ✓<br />
High<br />
reproducibility ✗ ✓ ✓ ✓ ✓ ✓<br />
Expensive<br />
✗ ✗ ✓ ✓ ✗ ✓
ATTENTION!<br />
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attendees only at the conference!<br />
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please register for the conference<br />
at<br />
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