Reporting form ERA-PG mid-term - Plant Stress Physiology

PLANT KBBE 2008
Project mid-term report form
A. Project
Project title, acronym and reporting period are given. In case of changes, indicate these using another colour.
Project title
Homeostasis and Transport of Iron – improving Plant Productivity and Growth
Project acronym HOT IRON – PLANT PROGROW
Start date
01.03.2009 (* please note that P1, P2, P3 and P5 started later)
Reporting period first 18 month of each project, for details see marks (*) below
Reporting date 25.04.2011
B. Consortium
Partner 1
* project start 01.05.2009, reporting period 01.05.09 - 31.10.10
(coordinator)
Name of grantholder PD Dr. Katrin Philippar
Institution
Ludwig-Maximilians-University München, LMU
Address Department Biology I, Plant Biochemistry and Physiology, Großhaderner Str. 2-4,
D-82152 Planegg-Martinsried
Country
Germany
Phone +49 (0)89 2180 74759
E-mail
philippar@lrz.uni-muenchen.de
Research team
Staff members and students
working on the project
Team leader / scientist /
postdoc / technician / PhD
student / MSc student /
other : ..
Person months
assigned to
project
Dr. Katrin Philippar Team leader, Coordinator 9 no
Dr. Daniela Duy Postdoctoral Scientist 18 yes
Karl Mayer Technician 3 no
Is this team member
appointed on project
grant ? yes / no
Partner 2 * project start 01.04.2009, reporting period 01.04.09 - 30.09.10
Name of grantholder Dr. Jean-François BRIAT
Institution
INRA
Address
BPMP-INRA-CNRS-University Montpellier-SupAgro, Place Viala,
Montpellier 34060 cedex 1
Country
France
Phone +33 (0)499 612 371
E-mail
briat@supagro.inra.fr
Research team
Staff members and studentes
working on the project
Team leader / scientist /
postdoc / technician / PhD
student / MSc student /
other : ..
Person months
assigned to
project
Dr. Jean-François Briat Team leader 11 no
Dr. Frédéric Gaymard Scientist 11 no
Dr. Françoise Cellier Scientist 15 no
Dr. Pierre Fourcroy Scientist 18 no
Brigitte Touraine Technician 9 no
Jossia Boucherez Technician 14 no
Dr. Damien Sudre Post-doc 12 yes
Is this team members
appointed on project
grant ? yes / no
Partner 3 * project start 01.05.2009, reporting period 01.05.09 - 31.10.10
Name of grantholder Prof. Dr. Nicolaus von Wirén
Institution
Leibniz Institute of Plant Genetics and Crop Plant Research, IPK
Address
Correnstraße 3, D-06466 Gatersleben
Country
Germany
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PLANT KBBE 2008
Project mid-term report form
Phone +49 (0)39482 5602
E-mail
vonwiren@ipk-gatersleben.de
Research team
Staff members and studentes
working on the project
Team leader / scientist /
postdoc / technician / PhD
student / MSc student /
other : ..
Person months
assigned to
project
Prof.Dr. Nicolaus von Wirén Project leader 1,5 no
Nicole Beate Schmid PhD student 18 yes
Seckin Eroglu PhD student 0 yes
Dr. Rongli Shi Postdoc 3 no
Is this team members
appointed on project
grant ? yes / no
Partner 4 * project start 01.03.2009, reporting period 01.03.09 - 31.08.10
Name of grantholder Prof. Dr. Javier Abadia
Institution
Estación Experimental de Aula Dei (CSIC)
Address
Dept. Plant Nutrition, Av. Montañana 1005, E-50080 Zaragoza
Country
Spain
Phone +34/(0)976-716056
E-mail
jabadia@eead.csic.es
Research team
Staff members and studentes
working on the project
Team leader / scientist /
postdoc / technician / PhD
student / MSc student /
other : ..
Person months
assigned to
project
Prof. Dr. Javier Abadia Team leader 10 no
Dr. Anunciación Abadía Scientist 10 no
Dr. Ana Álvarez Scientist 10 no
Dr. Yolanda Gogorcena Scientist 10 no
Dr. Vázquez Reina Postdoc 10 no
Dr. Rubén Rellán Álvarez Postdoc 18 (1/8/09-31/8/10) yes
Jorge Rodríguez Celma PhD student 10 no
Giuseppe Lattanzio PhD student 10 no
Is this team members
appointed on project
grant ? yes / no
Partner 5 * project start 01.07.2009, reporting period 01.07.09 - 31.12.10
Name of grantholder José María García-Mina Freire
Institution
CIPAV R&D TIMAC AGRO ESPAÑA, ROULLIER GROUP
Address
Polígono Arazuri-Orcoyen, C/C, No. 32. 11407, Orcoyen (Navarra)
Country
Spain
Phone +34 948 324 500
E-mail
jgmina@timacagro.es
Research team
Staff members and studentes
working on the project
Team leader / scientist /
postdoc / technician / PhD
student / MSc student /
other : ..
Person months
assigned to
project
José Mª García-Mina Project leader 6,42 Yes
Angel Mª Zamarreño Researcher 4,42 Yes
Roberto Baigorri Researcher 6,72 Yes
Marta Fuentes Researcher 2,50 Yes
Eva Bacaicoa Researcher 13,80 Yes
Sara San Francisco Researcher 10,55 Yes
María Garnica Researcher 13,35 Yes
Javier Erro Researcher 3,50 Yes
Esther Casanova Researcher 4,95 Yes
Is this team members
appointed on project
grant ? yes / no
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PLANT KBBE 2008
Project mid-term report form
C. Project summary for public dissemination
Provide an updated project summary including the progress of the project of 150 – 300 words for
public dissemination. The summary could be published and can be used for other websites (e.g. the
sites of the national funding organisations) and within publications such as the mid-term report of the
PLANT KBBE 2008 programme.
Iron is one of the most abundant metals on the earth’s crust. However, its redox properties make it an
element with low bioavailability, particularly in basic soil. Approximately 30% of the world’s arable soils
are thus iron deficient and because plants represent the major source for iron in human nutrition
almost 25% of the world population is affected by iron deficiency, a leading cause of anemia. In plants,
iron deficiency results in severe chlorosis as well as reduced productivity and quality of crops. This
essential element is involved in several vital processes such as photosynthesis, respiration, sulphur
and nitrogen assimilation and chlorophyll catabolism. Nevertheless this redox metal has a high
reactivity and can generate the formation of reactive oxygen species (ROS). Therefore, iron
homeostasis has to be tightly regulated to prevent oxidative stress. The photosynthetic machinery,
which is localised in chloroplasts, requires high iron for electron transport. Thus, chloroplasts
represent a platform controlling iron homeostasis (transport and storage) at the cellular level.
However, molecular processes controlling iron homeostasis in chloroplasts are still unknown and
might affect plant productivity under iron excess or deficiency.
With the permease PIC1 (Fe-transport) and ferritin (Fe-storage) the project HOT IRON works on key
components of chloroplast iron homeostasis in the model plant Arabidopsis thaliana. It was shown that
function of both proteins is essential for plant productivity and seed yield. Coordinated screening of
mutants (transcriptomics, metabolomics, proteomics and phenotyping) under iron deficiency and
overload is used to identify new players involved in crosstalk between iron and ROS metabolism in
leaves. First results point to the involvement of a chloroplast ABC-transporter. For transfer to crop
species, the corresponding genes have been identified in peach and cucumber. Results will be used
to modify and improve processes controlling productivity, growth and yield of these crop species.
D. Progress report
D1. Achievement of planned objectives
List the objectives and estimate the current degree of completion of the objectives.
OBJECTIVES
(i)1: Microarray and mutant based screen to identify new
key players linking iron homeostasis and transport.
(i)2: Screening for membrane transporters of Fe III
chelates.
(i)3: Screening for genes improving growth of
Arabidopsis lines on a calcareous substrate.
(i)4: Identification of factors limiting iron
retranslocation and distribution during plant senescence.
(i)5: Subcellular localisation of the proteins identified in
screens (i)1-(i)4.
(ii)1: Phenotypical analysis of iron homeostasis mutants.
(ii)2: Characteristics of iron transport across membranes
analysed by SURFE 2 R technique.
(iii)1: Identification of orthologous genes in peach and
cucumber.
(iii)2: Changes at the transcriptional, protein and
metabolite levels of major iron homeostasis players
in iron-sufficient, iron-deficient and iron-rich plants.
(iii)3: Spatial localisation of iron in Fe-rich as well as
chlorotic materials.
PROGRESS TO OBJECTIVES
Screen completed and new genes
identified by April 2011.
cDNA library made and isolation of an
inositol transporter improving yeast growth
on low Fe. Approach stopped.
Screening on calcareous soil started in
April 2010 and is still in progress. 2
mutants isolated. Second screening on
Fe-deficient agar started in January 2011.
Fe retranslocation studies in senescing
barley completed.
Finished for first proteins, constantly
supplemented with new players.
Analysis finished for ferritin, pic1 mutants.
Ongoing analysis for new players.
Establishment of technique started in
September 2010.
Finished, constantly supplemented with
new players.
Transcriptomics finished, proteomics and
metabolomics are ongoing (possible finish
in December 2011).
Techniques are established, experiments
started.
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PLANT KBBE 2008
Project mid-term report form
(iii)4: Design of new iron fertilisers.
Design of new Fe fertilizers started, will
accompany project until the end of funding
period.
D2. Activities
Describe the activities that have been performed to meet the objectives given in the proposal. Indicate
for each activity the partners involved (PX, PY,..).
For consortia with three or four partners this item should not exceed two pages. Larger consortia may
use at maximum three pages.
(i)1: Screen to identify new key players linking iron homeostasis and transport.
- Microarray experiments on three different mutant plants under three different conditions have been
performed and analysed (P1, P2).
- A database has been constructed to integrate and distribute results (P1, P4).
- With the chloroplast membrane localised ABC transporter ATH12 a candidate, which could be
involved in iron chloroplast export was integrated into the project (P2).
(i)2: Screening for membrane transporters of Fe III chelates by yeast complementation.
- cDNA library made from Fe-deficient Arabidopsis plants and isolation of an inositol transporter gene
(AtINT1) improving yeast growth under Fe deficiency. However, knock-out and overexpression in
Arabidopsis without phenotype (P3).
- Establishment of screening conditions for Arabidopsis mutant lines on agar (P3).
(i)3: Screening for genes improving growth of Arabidopsis lines on a calcareous substrate.
- Screening conditions were optimised, screening started in April 2010 (P3).
- Selection of some promising candidate lines and confirmation of Fe deficiency-induced chlorosis by
Fe resupply (P3).
- Confirmation of observed phenotype on Fe-deficient agar media by chlorophyll determinations,
assessment of root morphology and Fe uptake analysis (P3).
(i)4: Identification of factors limiting iron retranslocation and distribution.
- Iron tracing in plants using stable Fe isotopes (P4).
- Xylem and phloem sap and root metabolomic and proteomic studies (P4, P5).
(i)5: Subcellular localisation of the proteins identified in screens (i)1-(i)4.
- Proteins (PIC1, FER, MATE, ABC transporter) have been localised by GFP and immunoblot
analysis (P1, P2).
(ii)1: Phenotypical analysis of iron homeostasis mutants.
- Phenotypes of iron homeostasis mutants (pic1, fer, ath12) have been analysed under Fe-sufficient,
Fe-deficient, and Fe-rich conditions (P1, P2, P3, P4).
- Due to difficulties with existing PIC1 mutants, we started generating conditional PIC1 mutants (P1).
(ii)2: Characteristics of iron transport across membranes analysed by SURFE 2 R technique.
- Since Oktober 2010, the SURFE 2 R technique is established in the laboratory of P1.
(iii)1: Identification of orthologous genes in peach and cucumber.
- Orthologous genes to key players in Fe homoeostasis and transport have been identified in peach
and integrated into the database (P4).
- In cucumber, genes corresponding to Fe-transport and homeostasis are used as controls in
experiments of P5.
(iii)2: Changes at the transcriptional, protein and metabolite levels of major iron homeostasis players in
iron-sufficient, iron-deficient and iron-rich plants.
- Conditions to grow plants under Fe-sufficiency, -deficiency, and –excess have been tested and
established for large scale analysis (P2, P3).
- Microarray analysis for transcript changes have been performed (P1, P2).
- Samples of the respective mutants have been collected and prepared for metabolic and proteomic
analysis (P1, P2, P4).
- Chloroplasts of plants grown in iron excess, iron deficiency and in control condition as well were
extracted and prepared for proteomic and ionomic approach (P2, P4).
- Chloroplast envelope proteins have been subjected to proteome analysis (P1, P4).
- The role of hormones in Fe-deficiency induced responses has been studied (P5).
(iii)3: Spatial localisation of iron in Fe-rich as well as chlorotic materials.
- Spatial localization of Fe in leaves has been studied using Perl’s stain and µ-PIXE techniques (P4).
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PLANT KBBE 2008
Project mid-term report form
(iii)4: Design of new iron fertilisers.
- Preliminary works to carry out tests with new Fe fertilizers were developed (P4, P5; starting date
month 18).
D3. Problems and changes in workplan
Describe any difficulties and problems that have hindered the achievement of the planned objectives
and any changes with respect to the original objectives and the workplan (maximum 1 page).
(i)2: Screening for membrane transporters of Fe III chelates by yeast complementation.
The workplan is behind schedule, because one PhD student left the project due to health reasons,
however, a new PhD candidate (S. Eroglu) has been found who started in October 2010.
Since the isolated gene AtINT1only yielded a strong phenotype in yeast but not in planta, the yeast
complementation approach was stopped and replaced by a screening approach for Arabidopsis
mutant collections on Fe-deficient agar.
(i)3: Screening for genes improving growth of Arabidopsis lines on a calcareous substrate.
Because only a limited number of seeds was provided by gene stocks (NASC Nottingham) for
screening Arabidopsis mutant pools a timely confirmation of phenotypes of potential candidates was
delayed, as seeds need to be amplified prior to detailed analysis (P3).
The screening procedures takes longer than expected, because composition of substrate is variable
and requires re-calibration of screening conditions when going from one batch to another and
remediation of chlorotic phenotypes by Fe resupply requires more experimental proofs (P3).
Due to the new recruitment of personnel, a cost-neutral prolongation is required to fulfil the workplan.
(i)4: Identification of factors limiting iron retranslocation and distribution during plant senescence.
Fe retranslocation experiments conducted in senescing barley plants and Fe analysis completed (P3).
(ii)1: Phenotypical analysis of iron homeostasis mutants.
Large scale analysis of existing PIC1 knockout and overexpressing lines (Fe-import into chloroplasts)
proofed to be inefficient due to very strong chlorosis and death of mature plants. Therefore, the
generation of conditional PIC1 mutants is under way and mutants of the potential fe-exporter from
chloroplasts ATH12 were integrated into the study.
(ii)2: Characteristics of iron transport across membranes analysed by SURFE 2 R technique.
Because of the insolvency of the private sub-contractor IonGate, the experiments with the SURFE 2 R
technique could not be performed as planned and the workplan was delayed by about one year. Since
P1 has bought and installed a SURFE 2 R modul in September 2010, experimental conditions are now
established in the lab of P1.
D4. Collaboration, human mobility and training within the consortium
- Describe how coordination takes place (coordination meetings, communications, etc.)
- Describe the added value accomplished by collaboration among partners of the consortium. Refer
explicitly to joint milestones and deliverables produced during the reporting period.
- Describe any sharing of facilities within the consortium.
- Describe any exchange of researchers and trainees between partners of the consortium.
- Coordination of meetings, communications and experiments constantly takes place via Email contact
of the PIs and researchers. During the reporting period we organised two internal meetings for
exchange of experimental experiences and reports of results (Munich, 22.10.2009; Budapest,
01.07.2010). All scientists in addition participated at the 15 th International Symposium on Iron Nutrition
and Interaction in Plants, 26-30.06.2010 in Budapest. To exchange data and results an internal
webpage and database have been created.
- The added value accomplished by the partners is demonstrated by the intense collaboration
regarding sample exchange (genotypes, extracts, subcellular preparations) and use of advanced
analytical methods. For example P2, P3 have established plant growth conditions for parallel large
scale analyses, which are performed on the transcriptomic and proteomic/metabolomic levels by
partners P1 and P4/P5, respectively. On the other hand we are able to merge and use research
experience from the molecular (P1, P2), functional (P3) and applied fields (P4, P5).
- Facilities for large scale microarray (P1), metabolite (P4, P5), and protein (P4) analyses are shared
for experiments within the project.
- Short stay (1 week) of R. Rellán (P4) in P2’s lab in Montpellier.
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PLANT KBBE 2008
Project mid-term report form
D5. Other collaborations directly related to the project
- Describe the collaborations with other parties than consortium members.
- Describe any sharing of facilities with parties other than consortium members.
- Describe any exchange of researchers and trainees with other parties than consortium members.
-P4 collaborated with Dr. Ferenc Fodor’s lab (Eotvos Lorand University, Budapest) in the proteomic
characterization of thylakoid membranes affected by Fe deficiency.
-P4/P1/P2 collaborate (ongoing experiments) with Dr. Oliver Fiehn (U.C. Davis, USA) in metabolomic
analysis.
E. Dissemination
E1. Project-derived peer-reviewed publications and patents
Reference
1 Orera I, Rodríguez-Castrillón JA, Moldovan M, García-Alonso JI,
Abadía A, Abadía J, Álvarez-Fernández A (2010) Using a dualstable
isotope tracer method to study the uptake, xylem transport
and distribution of Fe and its chelating agent from stereoisomers of
a xenobiotic Fe(III)-chelate used as fertilizer in Fe-deficient
Strategy I plants. Metallomics 2, 646-657
(doi:10.1039/C0MT00018C)
2 El-Jendoubi H, Melgar JC, Álvarez-Fernández A, Sanz M, Abadía
A, Abadía J (2011) Setting good practices to assess the efficiency
of iron fertilizers. Plant Physiol Biochem,
(doi:10.1016/j.plaphy.2011.02.013)
3 Bacaicoa E, Mora V, Zamarreño AM, Fuentes M, Casanova E,
García-Mina JM (2011) Auxin: A major player in the shoot-to-root
regulation of root Fe-stress physiological responses to Fe
deficiency in cucumber plants. Plant Physiol Biochem,
(doi:10.1016/j.plaphy.2011.02.018)
4 Abadía J, Vázquez S, Rellán-Álvarez R, El-Jendoubi H, Abadía A,
Álvarez-Fernández A, López-Millán AF (2011) Towards a
knowledge-based correction of iron chlorosis. Plant Physiol
Biochem, (doi:10.1016/j.plaphy.2011.01.026)
5 Duy, D, Stübe, R, Wanner, G, and Philippar, K. (2011). The
chloroplast permease PIC1 regulates plant growth and
development by directing homeostasis and transport of iron. Plant
Physiology 155, 1709-1722 (doi/10.1104/pp.110.170233).
6 Rodríguez-Celma J, Lattanzio G, Grusak MA, Abadía A, Abadía J,
López-Millán AF (2011) Changes in the protein profile of Medicago
truncatula roots in direct and carbonateinduced Fe deficiency
conditions: increases in riboflavin synthesis and alterations in C/N
metabolism. J Proteom Res (doi:10.1021/pr2000623)
Type of publication
(peer-reviewed
article / patent)
peer-reviewed
article
peer-reviewed
article
peer-reviewed
article
peer-reviewed
article
peer-reviewed
article
peer-reviewed
article
Partners
involved
(PX,PY,..)
P4
P4
P5
P4
P1
P4
E2. Other dissemination
Reference
1 Rellán-Álvarez R, Andaluz S, López-Millán AF, Fiehn O, Álvarez-
Fernández A, Abadía J. Changes in the proteomic and
metabolomic profiles of Beta vulgaris root tips in response to iron
deficiency and resupply. XVI International Plant Nutrition
Colloquium (Sacramento, CA, US, 26-30 August 2009).
Type of publication
(poster abstract/
article in non-peer
reviewed science
journal /article in
newspaper /
interview / …)
Keynote Lecture
Partners
involved
(PX,
PY,..)
P4
6 / 8

PLANT KBBE 2008
Project mid-term report form
2 Briat JF (2009). Mechanisms of iron homeostasis in plants and
their regulations. XVI International Plant Nutrition Colloquium
(Sacramento, CA, US, 26-30 August 2009).
Keynote Lecture
3 Rellán-Álvarez R, Giner-Martínez-Sierra J, Orduna J, Orera I, Oral Presentation
Rodríguez-Castrillón JA, García-Alonso JI, Abadía J, Álvarez-
Fernández A. Iron is transported as a tri-Fe(III), tri-citrate complex
in plant xylem sap. XVIII Reunión de la Sociedad Española de
Fisiología Vegetal-X Congreso Hispano-Luso de Fisiología
Vegetal 5 (Zaragoza, España, 8-11 September 2009)
4 Briat JF (2009). Iron Nutrition in Plants. XI Congrès Hispano Keynote Lecture
Portugais de Physiologie Végétale. XVIII Reunión de la Sociedad
Española de Fisiología Vegetal-X Congreso Hispano-Luso de
Fisiología Vegetal 5 (Zaragoza, España, 8-11 September 2009)
5 Stübe R, Duy D, Philippar K. Homeoastasis and Transport of Iron Poster
in Chloroplasts. 23. Congress Plant Molecular Biology 23.-26.
February 2010, Dabringhausen, Germany
6 Abadía J, Vazquez S, Rellán-Álvarez R, Álvarez-Fernández A, Keynote Lecture
López-Millán AF, Abadía A. Towards a knowledgebased
correction of iron chlorosis. 15th International Symposium on Iron
Nutrition and Interactions in Plants (Budapest, Hungary, 26-30
June, 2010).
7 Briat JF, Arnaud N, Duc C, Ravet K, Gaymard F (2010).
Keynote Lecture
Integration of iron homeostasis with light and oxidative stress
signalings. 15th International Symposium on Iron Nutrition and
Interactions in Plants (Budapest, Hungary, 26-30 June, 2010).
8
9
10
11
12
13
14
15
16
17
Sudre D, Jasinski M, Gaymard F, Bovet L, Briat JF, Martinoia E
(2010) AtABCB29 / AtATH12 : a new member of ABC transporter
protein family involved in metal transport across chloroplast
membrane.
El Jendoubi H, Melgar JC, Abadía A, Álvarez-Fernández A,
Abadía J. Do's and do not's when assessing the efficacy of iron
fertilizers.
Rellán-Álvarez R, El Jendoubi H, Rodríguez-Celma J,
Wohlgemuth G, Abadía A, Fiehn O, López-Millán AF, Abadía J,
Álvarez-Fernández A. Delving into iron deficiency metabolomics.
Solti A, Basa B, Fodor F, Abadía J, Sárvári É. Effect of Cd(II),
Zn(II) and Mn(II) on chloroplast iron uptake.
García-Mina JM, Bacaicoa E, Zamarreño AM, Fuentes M,
Casanova E, Mora V, Yvin JC. Experimental evidence for the
important role of IAA in the expression of iron deficiency stress
root responses in cucumber plants.
Fuentes M, Ortuño MF, Bacaicoa E, Fernandez E, Perez-
Sarmiento F, Conejero W, Baigorri R, Torrecillas A, Yvin JC,
Garcia-Mina JM. A new class of hetero-molecular natural ironchelates
as efficient correctors of iron deficiency in fruit trees
cultivated in alkaline and calcareous soils.
Duy D, Stübe R, Philippar K Homeoastasis and Transport of Iron
in Chloroplasts
López-Millán A-F, Rodríguez-Celma J, Abadía A, Grusak MA,
Abadía J. Effects of Fe deficiency on the riboflavin
synthesis pathway in Medicago truncatula plants
Vázquez S, Abadía A, Abadía J. Micro-localization of iron in irondeficient
and iron-sufficient sugar beet leaves
Duy D, Stübe R, Philippar K. PIC1, a permease mediating
homeostasis and transport of iron in chloroplasts
Oral Presentation
Oral Presentation
Oral Presentation
Oral Presentation
Oral Presentation
Oral Presentation
Oral Presentation
Poster
Poster
Poster
P2
P4
P2
P1
P4
P2
P2
P4
P4
P4
P5
P5
P1
P4
P4
P1
All: 15th International Symposium on Iron Nutrition and
Interactions in Plants (Budapest, Hungary, 26-30 June, 2010).
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PLANT KBBE 2008
Project mid-term report form
18 Rellán-Álvarez R, Abadía J, Álvarez-Fernández A. Iron speciation
in plant xylem sap using LC-ESI-TOFMS. 6th International
Franco-Spanish Workshop on Bio-inorganic Analytical Chemistry
(Pau, France, Sept 2010)
19 von Wirén, N. “Identification of genes and mechanisms involved in
nutrient acquisition and homeostasis” XVII. Congress of the
Federation of European Societies of Plant Biology (FESPB),
Valencia, Spain
Oral Presentation
Invited Talk
P4
P3
F. Brief financial report
F1. Expenditures of the granted budgets (months 1-18)
Person Type Personnel Consumabl Travel (€) Equipment Total costs
months (postdoc /..) costs (€) es (€)
(€) (€)
P1 18 Postdoc 83.750 5.490 3.500 38.895 131.635
P2 12
33
Postdoc
Team staff
41.000 12.063
24.126
3.770
7.541 15.000
56.833
46.667
P3 18 PhD student 28.184 18.242 2.343 48.769
P4 17 Postdoc 52.056 4.125 2.273 32.732 91.186
P5 3,31 Team staff 11.135 24.431 4.022 59.697 99.285
Total 216.125 88.477 23.449 146.324 474.375
F2. Own contributions (months 1-18)
Person
months
P1 9
3
Type
(postdoc /..)
Team leader
Technician
Personnel
costs (€)
49.500
8.400
Consumabl
es (€)
7.500
Travel (€)
Equipment
(€)
Total costs
(€)
65.400
P2 33 Team staff 426.234 426.234
P3 1,5 Team leader 11.900
2 Postdoc 9.280 5.500 26.680
P4 38 Team staff 354.728 5.000 500 360.228
P5 62,89 Team staff 211.572 11.904 1.596 1.657 226.729
Total 1.071.614 29.904 2.096 1.657 1.105.271
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