Genetics characterization, nutritional and phytochemicals potential of gedi leaves (Abelmoschus manihot (L.) Medik) growing in the North Sulawesi of Indonesia as a candidate of poultry feed
Gedi, local name of Abelmoschus manihot (L.) Medik was used by local people in Northern Sulawesi-Indonesia as vegetable, because of its medicinal properties. The potency of gedi leaves in broiler diet has not been reported in literatures. The objective of this research was to investigate a genetic diversity of gedi commonly consumed as a gourmet cuisine in the North Sulawesi of Indonesia, and exploring the potential of this plant as a herb plant for a candidate of poultry feedstuff. Eight morphologically different gedi leaves (GH1, GH2, GH3, GH4, GH5, GH6, GM1 and GM2) that grow in Manado area, North Sulawesi of Indonesia were collected and identified. The leaves were extracted for DNA isolation followed by PCR and DNA sequencing analysis. During DNA isolation, 3 of 6 GH (GH4, GH5, GH6) were discontinued because of difficulty in separating the mucilage properties. Following PCR analysis, GH2 and GH3 did not produce bands and consequently were excluded from further analysis. In addition to that, chemical analysis was also performed to determine the phytochemical and nutritional contents .The results indicated that all gedi leaf samples showed similarity (99%) to species member of Abelmoschus manihot, and tribe of Malvaceae. In terms of proximate analysis, gedi leaves showed high crude protein (18.76 - 24.16%) and calcium (2.92-3.70%) content. Also, showed high crude fibre (13.06-17.53%). Together with the presence of alkaloid and steroidal saponin gedi leaves may offer beneficial effects as poultry feedstuff to a special production trait such as cholesterol-less meat.
Gedi, local name of Abelmoschus manihot (L.) Medik was used by local people in Northern Sulawesi-Indonesia as vegetable, because of its medicinal properties. The potency of gedi leaves in broiler diet has not been reported in literatures. The objective of this research was to investigate a genetic diversity of gedi commonly consumed as a gourmet cuisine in the North Sulawesi of Indonesia, and exploring the potential of this plant as a herb plant for a candidate of poultry feedstuff. Eight morphologically different gedi leaves (GH1, GH2, GH3, GH4, GH5, GH6, GM1 and GM2) that grow in Manado area, North Sulawesi of Indonesia were collected and identified. The leaves were extracted for DNA isolation followed by PCR and DNA sequencing analysis. During DNA isolation, 3 of 6 GH (GH4, GH5, GH6) were discontinued because of difficulty in separating the mucilage properties. Following PCR analysis, GH2 and GH3 did not produce bands and consequently were excluded from further analysis. In addition to that, chemical analysis was also performed to determine the phytochemical and nutritional contents .The results indicated that all gedi leaf samples showed similarity (99%) to species member of Abelmoschus manihot, and tribe of Malvaceae. In terms of proximate analysis, gedi leaves showed high crude protein (18.76 - 24.16%) and calcium (2.92-3.70%) content. Also, showed high crude fibre (13.06-17.53%). Together with the presence of alkaloid and steroidal saponin gedi leaves may offer beneficial effects as poultry feedstuff to a special production trait such as cholesterol-less meat.
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M<strong>and</strong>ey et al., 2014<br />
Harborne JB. 1987. Metode Fitokimia, Penuntun Cara<br />
Modern Menganalisis Tumbuhan.Translater:<br />
Padmaw<strong>in</strong>ata K dan I. Sudiro I. Institut Teknologi<br />
B<strong>and</strong>ung, B<strong>and</strong>ung.<br />
Ja<strong>in</strong> PS, Bari SB <strong>and</strong> Surana SJ. 2009. Isolation <strong>of</strong><br />
stigm<strong>as</strong>terol <strong>and</strong> ý-sitosterol from petroleum e<strong>the</strong>r<br />
extract <strong>of</strong> woody stem <strong>of</strong> <strong>Abelmoschus</strong> <strong>manihot</strong>.Asian J.<br />
<strong>of</strong> Biological Sci. 2(4):112-117, from http://<br />
w w w . s c i a l e r t . n e t / q d i r e c t . p h p ? /<br />
doi=ajbs.2009.112.117&l<strong>in</strong>kid=pdf.<br />
Ja<strong>in</strong> PS <strong>and</strong> Bari SB. 2010. Anti-<strong>in</strong>flammatory activity<br />
<strong>of</strong> <strong>Abelmoschus</strong> <strong>manihot</strong> extracts. Intern. J.<br />
<strong>of</strong>Pharmacology. 6 (4):505-509. http://www.scialert.net/<br />
qdirect.php?.doi=ijp. 2010.505.509&l<strong>in</strong>kid=pdf.<br />
Ja<strong>in</strong> PS, Todarwal AA.Bari SB, Sanjay JS. 2011..<br />
Analgesic activity <strong>of</strong> <strong>Abelmoschus</strong> <strong>manihot</strong> extracts.<br />
Intern. J. <strong>of</strong> Pharmacology. 7(6):716-720.http://<br />
w w w . s c i a l e r t . n e t / f u l l t e x t / ?<br />
doi=ijp.2011.716.720&org=11.<br />
Wang XR, Wang ZQ, Li Y. 1981. Studies on <strong>the</strong><br />
chemical constituents <strong>of</strong> <strong>Abelmoschus</strong> <strong>manihot</strong> L. Medic.<br />
Acta Bot. S<strong>in</strong>., 23(3):222-227.<br />
Wang XR, Zhou ZH, Du AQ, Huang ZM. 2004.<br />
Studies on <strong>the</strong> flavonol constituents <strong>of</strong><br />
<strong>Abelmoschus</strong><strong>manihot</strong> L. Medic. Ch<strong>in</strong>. J. Nat. Med.,<br />
2(2):91-93.<br />
W<strong>in</strong>disch W, Schedle K, Plitzner C, Kroismayr A.<br />
2008. Use <strong>of</strong> phytogenic products <strong>as</strong> <strong>feed</strong> additives for<br />
sw<strong>in</strong>e <strong>and</strong> <strong>poultry</strong>. J. <strong>of</strong> Animal Sci.86(14Suppl.):E140-<br />
E148.http:// www.journal<strong>of</strong>animalscience.org/<br />
content/86/14Suppl/E140.<br />
Yang CJ, Yang IY, Oh DH, Bae IH, Cho SG, Kong<br />
IG, Uuganbayar D , Nou IS, Choi KS. 2003. Effect <strong>of</strong><br />
green tea by-product on performance <strong>and</strong> body<br />
composition <strong>in</strong> broiler chicks. Asian-Australian J. <strong>of</strong><br />
Anim. Sci., 16(6):867-872. From http://www.aj<strong>as</strong>.<strong>in</strong>fo/<br />
Editor/manuscript/upload/16_128.pdf.<br />
Khatun MH, Rahman MA, Bisw<strong>as</strong> M, Ul Islam MA.<br />
2010. In vitro study <strong>of</strong> <strong>the</strong> effects <strong>of</strong> viscous soluble<br />
dietary fibers <strong>of</strong> <strong>Abelmoschus</strong> esculentus L <strong>in</strong> lower<strong>in</strong>g<br />
<strong>in</strong>test<strong>in</strong>al glucose absorption. Bangladesh Pharmaceutical<br />
J., 13(2):35-40.<br />
Pr<strong>as</strong>ad K, Janve B, Sharma RK, Pr<strong>as</strong>ad KK. 2010.<br />
Compositional <strong>characterization</strong> <strong>of</strong> traditional medic<strong>in</strong>al<br />
plants: Chemo-metric approach. Archives <strong>of</strong> Applied<br />
Sci. Research. 2(5):1-10.<br />
Preston SR. 1998. Aibika/Bele. <strong>Abelmoschus</strong> <strong>manihot</strong><br />
(L.) <strong>Medik</strong>. International Plant Genetic Resources<br />
Institute. Rome, Italy.97 pages.<br />
Puel C, Ma<strong>the</strong>y J, Kati-Coulibaly S, Davicco MJ,<br />
Lebecque P, Chanteranne B, Horcajada MN,<br />
Coxam V. 2005. Preventive effect <strong>of</strong> <strong>Abelmoschus</strong><br />
<strong>manihot</strong> (L.) <strong>Medik</strong>. on bone loss <strong>in</strong> <strong>the</strong> ovariectomised<br />
rats. J. Ethnobotanical. 99(1):55-60.<br />
Sarwar M, Attitallia IH, Abdollahi M. 2011. A review<br />
on <strong>the</strong> recent advances <strong>in</strong> pharmacological studies on<br />
medic<strong>in</strong>al plants; animal studies are done but cl<strong>in</strong>ical<br />
studies needs complet<strong>in</strong>g. Asian J. <strong>of</strong> Animal <strong>and</strong><br />
Veter<strong>in</strong>ary Advances. 6(8):867-883.<br />
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