Cover Conference-at-a-Glance Short Courses/Plenary Keynote <strong>Sequencing</strong> Strategies for Success Dynamics of the Microbiome on Health and Disease Single-Cell <strong>Sequencing</strong> <strong>Sequencing</strong> Data Analysis and Interpretation Sponsor & Exhibit Opportunities Hotel & Travel Information Registration Information Click Here to Register Online! Healthtech.com/<strong>Sequencing</strong> Register by July 12 and Save up to $250 Cambridge Healthtech Institute 250 First Avenue, Suite 300 Needham, MA 02494 www.healthtech.com Inaugural NG X Single-Cell <strong>Sequencing</strong> Meeting the Challenge One Cell at a Time August 20-21, <strong>2013</strong> Over the past decade, NGS technologies have moved at a rapid pace, dramatically reducing costs, and making genome sequencing more routine. What was once unthinkable is now possible. However, most genomes are still sequenced from DNA extracted from multiple cells, which misses differences between cells that could be crucial in controlling gene expression, cell behavior, and drug response. Still, challenges for single-cell sequencing remain, including cell isolation, DNA amplification, and bioinformatics. As the techniques are being refined, subtle differences between cells, such as the tiny genomic rearrangements, will emerge. CHI’s Single- Cell <strong>Sequencing</strong> conference focuses on the links between cell variation in tissues and organ function and further elucidates the origins of diseases. Tuesday, August 20 12:00 pm Main Conference Registration Sponsored by 12:30 From Reads to Variants: Ten-Fold Reduction in Time and Cost with Improved Accuracy Rupert Yip, Ph.D., Director, Product Marketing, Bina Technologies Alignment and variant calling of raw NGS reads has been plagued by expensive HPC hardware and the bioinformatics personnel to support and maintain home-grown, opensource secondary analysis solutions. Such solutions can take up to weeks and $1000s per analysis. We present a genomic analysis platform that reduces, by ten-fold, the time and cost for secondary analysis while improving accuracy compared to standard pipelines. Our innovative model reduces costs by ten-fold while preventing hardware obsolescence. Tuesday, August 20 »» Plenary Keynote Session 2:00 Chairperson’s Opening Remarks Toby Bloom, Ph.D., Deputy Scientific Director, Informatics, New York Genome Center 2:10 A Revolution in DNA <strong>Sequencing</strong> Technologies: Challenges and Opportunities Jeffery A. Schloss, Ph.D., Director, Division of Genome Sciences, National Human Genome Research Institute, National Institutes of Health The initial sequencing of the human genome spurred an appetite for much more human 10 sequence information to better understand the contributions of human sequence variation to health and disease. However, despite dramatic reductions during the Human Genome Project, the cost of sequencing was clearly too high to collect the very large numbers of human and numerous other organism genome sequences needed to achieve that understanding. In 2004, NHGRI launched parallel programs to reduce the cost of sequencing a mammalian genome initially by two (in five years), and eventually by four orders of magnitude (in ten years). This presentation will summarize the technologies that are in high-throughput use to produce stunning amounts of sequence and related data and novel biological insights, and will emphasize technologies currently emerging and on the horizon that may provide human genome sequence data with the nature, quality, cost and turnaround time needed for applications in research and medicine. 2:50 RNA is Everywhere: Characterizing the Spectra and Flux of RNA in Mammalian Circulation David Galas, Ph.D., Principal Scientist, Pacific Northwest Diabetes Research Institute The discovery of foreign RNA in blood and tissues of humans and mice raises many questions, including its origins, the mechanisms of its transport and stability and what, if any, functions it has. I will discuss what we know about circulating exRNA in human plasma and the use of NGS in the exploration of this new area of investigation in biology and medicine. 3:30 Refreshment Break in the Exhibit Hall with Poster Viewing 4:15 Genomics and the Single Cell Sherman Weissman, Ph.D., Sterling Professor of Genetics and Medicine, Yale University School of Medicine Studies of single cells are being approached by widely different methods, principally either florescence microscopy including super-high resolution methods, cloning and expansion >>
Cover Conference-at-a-Glance Short Courses/Plenary Keynote <strong>Sequencing</strong> Strategies for Success Dynamics of the Microbiome on Health and Disease Single-Cell <strong>Sequencing</strong> <strong>Sequencing</strong> Data Analysis and Interpretation Sponsor & Exhibit Opportunities Hotel & Travel Information Registration Information Click Here to Register Online! Healthtech.com/<strong>Sequencing</strong> Register by July 12 and Save up to $250 Cambridge Healthtech Institute 250 First Avenue, Suite 300 Needham, MA 02494 www.healthtech.com >