CVM facilities and equipment - University of Minnesota College of ...
CVM facilities and equipment - University of Minnesota College of ...
CVM facilities and equipment - University of Minnesota College of ...
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<strong>College</strong> <strong>of</strong> Veterinary Medicine<br />
The <strong>College</strong> <strong>of</strong> Veterinary Medicine houses a variety <strong>of</strong> <strong>facilities</strong> <strong>and</strong> laboratory <strong>equipment</strong><br />
that is available for <strong>CVM</strong> researchers to share or use for a fee. Please check with the contact<br />
person listed for availability <strong>of</strong> the <strong>equipment</strong>.<br />
<strong>CVM</strong> Facilities<br />
FLOW CYTOMETRY FACILITY (Rm 155, ASVM). This fee-for-service facility<br />
contains one FACSCanto equipped for multiplex fluorescent color acquisition/analysis on a<br />
HP PC, <strong>and</strong> is networked with two Apple PCs equipped with CellQuest <strong>and</strong> FlowJo for data<br />
analysis, Micros<strong>of</strong>t <strong>of</strong>fice, <strong>and</strong> statistical s<strong>of</strong>tware; <strong>and</strong> one FLUOstar OPTIMA: A fully<br />
automated microplate based reader that performs fluorescence intensity, time-resolved<br />
fluorescence, high-performance luminescence (flash <strong>and</strong> glow), <strong>and</strong> absorbance, which is<br />
operated through a Dell PC.<br />
Contact Person: Bruce Walcheck<br />
Phone: 624-2282<br />
E-mail: walch003@umn.edu<br />
FILM PROCESSOR (Rm 38, ASVM). The CP1000 tabletop film processor is a fee-forservice<br />
instrument that performs high-quality processing <strong>of</strong> film sizes ranging from 4” X 4”<br />
up to 14” X 36”.<br />
Contact Person: Bruce Walcheck<br />
Phone: 624-2282<br />
E-mail: walch003@umn.edu<br />
ZEISS AXIOVERT INVERTED FLUORESCENCE MICROSCOPY STATION (Rm 201,<br />
ASVM). The Axiovert 200 inverted microscope with the Cell Observer system for live cell<br />
imaging <strong>of</strong>fers an on-site system that is flexible, exp<strong>and</strong>able <strong>and</strong> user-friendly due to its<br />
many amenities. These include: (i) eye-level LCD display with magnification, shutter<br />
position, function information; (ii) features such as Aqua Stop <strong>and</strong> Ergo tube which protect<br />
expensive optics <strong>and</strong> provide comfortable use; (iii) sensitive fluorescence capability using the<br />
Light Trap system with large field <strong>of</strong> view; <strong>and</strong> (iv) digital imaging fordocumentation <strong>and</strong> 1-<br />
6 dimensional image construction.<br />
Contact Person: Laura Mauro<br />
Phone: 624-6274<br />
E-mail: mauro002@umn.edu<br />
GAIT ANALYSIS LABORATORY (Rm 231 VMC) with serial AMTI OR6-7-1000 force<br />
platforms recessed into the floor, Acquire s<strong>of</strong>tware on a dedicated computer for analysis <strong>of</strong><br />
the kinetic force platform data, 6 digital cameras <strong>and</strong> one color live camera for optical<br />
capture <strong>of</strong> body movements during gait <strong>and</strong> Peak Performance s<strong>of</strong>tware on a dedicated<br />
computer for analysis <strong>of</strong> the kinematic optical capture data. A General Electric Lunar<br />
Prodigy Advance dual energy xray absorptiometer that measures bone mineral density <strong>and</strong><br />
bone mineral content <strong>and</strong> has s<strong>of</strong>tware for total body measurement that is an effective tool<br />
for assessing secondary conditions that cause osteoporosis or osteopenia, disorders that affect
ody composition, as well as growth in immature animals. This technology allows a noninvasive<br />
measurement <strong>of</strong> skeletal bone status <strong>and</strong> lean <strong>and</strong> fat tissue components including<br />
percentage fat, lean tissue mass, etc. that can be used for monitoring <strong>of</strong> weight reduction,<br />
treatment response in primary <strong>and</strong> secondary hyperpararthyroidism, Cushing’s disease,<br />
cachexic disorders <strong>and</strong> malabsorptive syndromes. There is also a treadmill with a dedicated<br />
computer that uses telemetry to assess cardiac function (Dr. Ferasin).<br />
Contact person: Liz Pluhar<br />
Phone: 625-1162<br />
E-mail: pluha006@umn.edu<br />
Equipment Available in<br />
Individual Faculty Laboratories<br />
Equipment<br />
Anaerobic chamber<br />
Bioanalyzer system, 2100<br />
BioRad D-Code DGGE system<br />
BioRad Chel Mapper<br />
Centrifuge - Avanti J-25 high performance low-speed centrifuge<br />
Faculty Contact<br />
R. Singer<br />
singe024@umn.edu<br />
625-6271<br />
161 AS/VM<br />
R. Isaacson<br />
isaac015@umn.edu<br />
624-0701<br />
247 Vet.Sci.<br />
K. Reed<br />
reedx054@umn.edu<br />
624-1287<br />
168 AS/VM<br />
M. Najenga<br />
njeng001@umn.edu<br />
Centrifuge – Fisher 225, 24-position<br />
Centrifuge - preparative ultra – Beckman optimal LE-80K<br />
Cryotome<br />
ChemiDoc gel documentation system<br />
DNA analysis system - automated, Beckman CEQ 8000XL<br />
J. Collister<br />
colli066@umn.edu<br />
626-1206<br />
343 AS/VM<br />
M. Najenga<br />
C. Carlson<br />
carls099@umn.edu<br />
625-7717<br />
M. Najenga<br />
J. Mickelson<br />
micke001@umn.edu<br />
624-1246
164 AS/VM<br />
Electrophoresis chambers vertical <strong>and</strong> horizontal<br />
M. Murtaugh<br />
murta001@umn.edu<br />
625-6735<br />
245 Vet.Sci.<br />
Electrophoresis –<br />
Pulsed field gel – BioRad Chef DR2,<br />
Pulsed field gel - BioRad Chef DR3<br />
Pulsed field gel - BioRad Chef DR3<br />
Pulsed field gel – BioRad Chef DR2<br />
Evaporator/concentrator speed vac<br />
Force plate gait analysis system<br />
Isoelectric focusing column – LKB 110 ml preparative<br />
Laser capture microdissection instrument<br />
Lesion maker UGO DC<br />
Myograph system - automated<br />
D. Halvorson<br />
halvo002@umn.edu<br />
625-5292<br />
342 Vet.Sci.<br />
J. Bender<br />
bende002@umn.edu<br />
625-6203<br />
R. Singer<br />
singe024@umn.edu<br />
625-6271<br />
161 AS/VM<br />
R. Isaacson<br />
isaac015@umn.edu<br />
624-0701<br />
247 Vet.Sci<br />
M. Murtaugh<br />
murta001@umn.edu<br />
625-6735<br />
245 Vet.Sci.<br />
E. Pluhar<br />
pluha006@umn.edu<br />
625-1162<br />
D. Halvorson<br />
halvo002@umn.edu<br />
625-5292<br />
342 Vet.Sci.<br />
See <strong>University</strong><br />
<strong>facilities</strong> below<br />
J. Collister<br />
colli066@umn.edu<br />
626-1206<br />
343 AS/VM<br />
M. Kannan<br />
kanna001@umn.edu<br />
624-3757
137F Vet.Sci.<br />
pH meter <strong>and</strong> peristatic pumps<br />
M. Murtaugh<br />
murta001@umn.edu<br />
625-6735<br />
245 Vet.Sci.<br />
Pulmonary function measurement system<br />
Radiometric calcium imaging system<br />
M. Kannan<br />
kanna001@umn.edu<br />
624-3757<br />
137F Vet.Sci.<br />
Real time PCR –<br />
- ABI Prism 7000 sequence detection system M. Murtaugh<br />
murta001@umn.edu<br />
625-6735<br />
234 Vet.Sci.<br />
- Stratagene 3000 Y. Ji<br />
jixxx002@umn.edu<br />
624-2757<br />
234 Vet.Sci.<br />
- Stratagene 3000 – 66 AS/VM C. Lancto<br />
- Stratagene 3000 – 81 AS/VM lanct002@umn.edu<br />
625-7737<br />
Spectrophotometer – Beckman DV640<br />
Spectrophotometer – UV/Vis, Peltier temperature control<br />
Perkin Elmer<br />
Sonicator<br />
J. Mickelson<br />
micke001@umn.edu<br />
624-1246<br />
164 AS/VM<br />
R. Isaacson<br />
isaac015@umn.edu<br />
624-0701<br />
247 Vet.Sci<br />
M. Murtaugh<br />
murta001@umn.edu<br />
625-6735<br />
245 Vet.Sci.<br />
Telemetry system – acquisition system, 32-channel<br />
J. Collister<br />
- transmitters, 24-channel colli066@umn.edu<br />
- receivers, 24 channel 626-1206<br />
343 AS/VM<br />
Thermocyclers, 96-well<br />
J. Mickelson<br />
micke001@umn.edu<br />
624-1246<br />
164 AS/VM
R. Singer<br />
singe024@umn.edu<br />
625-6271<br />
161 AS/VM<br />
K. Reed<br />
reedx054@umn.edu<br />
624-1287<br />
168 AS/VM<br />
Thermocycler, Touchgene<br />
Ventilator – Harvard small animal 683<br />
K. Reed<br />
reedx054@umn.edu<br />
624-1287<br />
168 AS/VM<br />
J. Collister<br />
colli066@umn.edu<br />
626-1206<br />
343 AS/VM
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