Reagent Kit - Biokit

BIOKIT S.A.
08186 Llissà d'Amunt
(Barcelona) Spain
Phone: (34) 93 860 9000
Fax: (34) 93 860 9029
e-mail: biokit@biokit.com
SAFETY DATA SHEET
Code: 3000-1474
Date: 27-07-05 Rev.: 02
Pag.: 1 of 3
1. Identification of preparation
Product name: bioblot HCV
2. Composition/information on ingredients
Kit components:
Nitrocellulose strips: Strips of nitrocellulose incorporated with HCV recombinant structural and non-structural
antigens and two serum addition control (anti-human IgG and human IgG) bands.
Concentrate conjugate: Goat anti-human IgG antibodies conjugated with alkaline phosphatase. Contains
sodium azide.
Negative control: Inactivated human serum negative for HCV, HIV-1, HIV-2 antibodies and hepatitis B surface
antigen (HBsAg). Contains sodium azide and thimerosal.
Positive control: Inactivated human serum containing a high titre of HCV antibodies. Negative for hepatitis B
surface antigen (HBsAg) and for HIV-1, HIV-2 and HTLV-I/II antibodies. Contains sodium azide and thimerosal.
Washing solution: Concentrate Tris buffer containing Tween 20 and thimerosal.
Concentrate blotting buffer: Concentrate Tris buffer. Contains heat inactivated normal goat serum and
thimerosal.
Substrate: 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and nitroblue tetrazolium (NBT) solution.
Blotting powder: Powdered non-fat dry milk
3. Hazards identification
Positive and negative controls contain Sodium Azide (NaN 3) and Thimerosal.
Sodium azide CAS-Nº: 26628-22-8 EINECS-Nº: 247-852-1
Thimerosal CAS-Nº: 54-64-8 EINECS-Nº: 200-210-4
Harmful.
Concentrate conjugate contains Sodium Azide (NaN 3).
Sodium azide CAS-Nº: 26628-22-8 EINECS-Nº: 247-852-1
Harmful.
Concentrate blotting buffer and washing solution contain Thimerosal.
Thimerosal CAS-Nº: 54-64-8 EINECS-Nº: 200-210-4
Harmful.
Substrate contains 5-bromo-4-chloro-3-indolyl-phosphate and nitroblue tetrazolium.
5-bromo-4-chloro-3-indolyl-phosphate CAS-Nº: 102185-33-1 EINECS-Nº: None
Nitroblue tetrazolium CAS-Nº: 298-83-9 EINECS-Nº: 206-067-4
Harmful.
4. First aid measures
Inhalation: Remove from exposure. If breathing is difficult, obtain medical attention if necessary.
Eye contact: Rinse immediately with water for at least 15 minutes separating eyelid.
Skin contact: Wash off thoroughly with plenty of water. Remove and wash contaminated clothing.
Ingestion: Obtain medical attention
5. Fire-fighting measures
Extinguishing media: Use media in adaptation to materials stored in the immediate neighbourhood.
Special firefighting procedures: Wear self-contained breathing apparatus and protective clothing to prevent contact with
skin and eyes.
6. Accidental release measures
After spillage: Wipe up spills with inert absorbant tissues and place in a suitable container. Use protective gloves.

BIOKIT S.A.
08186 Llissà d'Amunt
(Barcelona) Spain
Phone: (34) 93 860 9000
Fax: (34) 93 860 9029
e-mail: biokit@biokit.com
SAFETY DATA SHEET
Code: 3000-1474
Date: 27-07-05 Rev.: 02
Pag.: 2 of 3
7. Handling and storage
Handling: The normal precautions for handling chemicals must be observed. Wash hands after handling.
Storage: Between +2ºC to 8ºC.
8. Exposure controls/personal protection
Respiratory protection: Good local ventilation.
Eye protection: Protective glasses.
Hand protection: One-way gloves (latex or plastic).
Use protective lab coat. Is recommended the use of safety pipette device.
9. Physical and chemical properties
Appearance:
Nitrocellulose strips: white plastic strip
Concentrate conjugate: colourless liquid
Negative control: straw liquid
Positive control: straw liquid
Washing solution: straw liquid
Concentrate blotting buffer: yellowish liquid
Substrate: light yellow liquid
Blotting powder: yellowish powder
Odour: Odourless
Boiling point: N/A
Melting point: N/A
Flash point: N/A
Ignition temperature: N/A
Explosion limits: N/A
Vapour pressure: N/A
Density: N/A
Viscosity: N/A
Solubility in water: soluble
10. Stability and reactivity
Conditions and materials to avoid: N/A
Hazardous reactions: N/A
Hazardous descomposition products: N/A
11. Toxicological information
Data not available.
12. Ecological information
Data not available.
13. Disposal considerations
Remains of samples, reagents and controls should be collected in a recipient for this purpose and autoclaved 1 hour at
121ºC.
Observe all Federal, State and local laws.
14. Transport information
Specials requirements: None

BIOKIT S.A.
08186 Llissà d'Amunt
(Barcelona) Spain
Phone: (34) 93 860 9000
Fax: (34) 93 860 9029
e-mail: biokit@biokit.com
SAFETY DATA SHEET
Code: 3000-1474
Date: 27-07-05 Rev.: 02
Pag.: 3 of 3
15. Regulatory information
According to 1999/45/EC Directive and 91/155/EEC Directive and following modifications.
Positive and negative controls:
Hazard symbol Xn Harmful
R-phrases
R22
Harmful if swallowed.
Concentrate conjugate:
Hazard symbol Xn Harmful
R-phrases
R22
Harmful if swallowed.
Concentrate blotting buffer and washing solution:
Hazard symbol Xn Harmful
R-phrases
R22
Harmful if swallowed.
Substrate:
Hazard symbol Xn Harmful
R-phrases
R20/21/22
Harmful by inhalation, in contact with skin and if swallowed.
16. Other information
Intended use: Qualitative enzyme immunoassay for the detection of antibodies to hepatitis C virus (HCV) in human serum
or plasma. It is intended for use as a more specific supplemental test on human serum or plasma specimens found
repeatedly reactive using screening procedures such as ELISA.
For IN VITRO diagnostic use.
Some reagents in this kit contain sodium azide as a preservative. Sodium azide has been reported to form lead or copper
azide in laboratory plumbing which may explode on percusion. Use proper disposal procedures.
Each donor unit used in the preparation of the controls of this kit was tested by an approved method for the presence of
the antibodies to HIV and HCV as well as for HBsAg and found to be negative. WARNING: Because no test method can
offer complete assurance that HIV, HCV, HBsAg or other infectious agents are absent, the controls of this kit should be
handled carefully.
The above information is believed to be correct, but does not purpose to be all inclusive and should be used only as a
guide. BIOKIT S.A. shall not be held for any damage resulting from handling or use of the product.