DUALEX SCIENTIFIC - Force-A

More documents

Recommendations

Info

DUALEX ® SCIENTIFIC, AN INNOVATIVE TOOL Resulting from a CNRS (National Center for Scientific Research) research team and the University of Paris-Sud in Orsay, this new leaf-clip can simultaneously and accurately monitor the chlorophyll content in leaves and flavonoid content in the epidermis. Versatile, it is dedicated to plant science and agronomy. Applied equally on monocots, dicots or perennials, this tool is simple to use. Measurements are instantaneous and non-destructive. They require no prior calibration, and no sample preparation. Therefore, measurements can either be done in the laboratory or in the field, in all conditions of temperature and ambient light. FLAVONOID measurement Epidermal flavonoids are absorbing UV light, and therefore they screen the chlorophyll underneath. FORCE-A uses this role of flavonoids as natural UV-filters to identify and quantify these molecules. CHLOROPHYLL measurement The leaf chlorophyll content can rapidly and accurately be assessed from light transmission. A first wavelength very close to the red 3 quantifies the chlorophyll and a second in the near infrared 4 can take into account the effects of leaf structure. Flavonoid absorption Absorption of leaves Chlorophyll Fluorescence 1 UV LED VIS 2 3 NIR 4 200 300 400 500 600 700 800 900 A wavelength in the UV 1 is compared to a second wavelength in the red 2 . Both wavelengths excite the fluorescence of chlorophyll, but only UV is affected by the presence of flavonoids. By using these two wavelengths, the DUALEX ® SCIENTIFIC calculates a numeric value directly correlated with chlorophyll content in the measured sample. Flavonoid absorbance = Log Infrared Fluo. excited by Red Infrared Fluo. excited by UV Chlorophyll content = Infrared Trans. Red Trans. Red Trans. The difference in chlorophyll fluorescence measured in the infrared is thus directly proportional to the amount of flavonoids present in the epidermis of the leaf. Sampling wavelength: UV-A EPIDERMIS: Rich in flavonoids MESOPHYLL: Rich in chlorophyll Reference wavelength: Red CHLOROPHYLL INDEX (DUALEX INDICE CHLOROPHYLLE (DUALEX® ® 4) 4) 2009 season Partnership CIVC / FORCE-A / ESE Chardonnay and Pinot Noir (N=109) Differential measurement of fluorescence emitted by chlorophyll CHLOROPHYLLE Chlorophyll /
Strengths CEREALS n LIGHTWEIGHT AND COMPACT Very light (only 220 g, battery included) and compact (it fits into the hand), the DUALEX ® SCIENTIFIC is easily portable and can be used frequently. Its ergonomics is specifically designed to measure leaves from 0.5 to over 16 cm wide. n SIMPLE MEASUREMENTS In automatic mode, the measurement is recorded each time the device detects the presence of a leaf (manual triggering also available). VINE n ADAPTED TO EXPERIMENTATION The DUALEX ® SCIENTIFIC offers the option to remove the last measurement, to manage measurement groups (three levels of classification), and to record more than 10.000 multiparametric measurements with additional information: date, time, group number and GPS position (meter accuracy). n EASY DATA MANAGEMENT Data can be downloaded via USB in a format compatible with any data processing software. Free access (up to 8 cm) n LONG AUTONOMY Its rechargeable Li-ion high-capacity battery is guaranteed for 1000 charging cycles. Thanks to the technology used, the device can achieve up to 25.000 measurements for a charging time of maximum 4 hours. EXAMPLE OF APPLICATION: MONITORING OF NITROGEN STATUS A NEW INDEX: THE NBI ® Several years of research and experimentation showed that polyphenols, specifically flavonoids, are indicators of nitrogen status of plants. Indeed, when a plant is under optimal conditions (1), it favours its primary metabolism and synthesises proteins (nitrogen-containing molecules) containing chlorophyll, and some flavonoids (carbon-based secondary compounds). On the contrary, in case of nitrogen deficiency (2), the plant directs its metabolism towards an increased production of flavonoids like shown on the diagram below: (1) Optimal conditions: Photosynthesis Chlorophyll Flavonoids (2) Nitrogen deficiency: Secondary metabolism By using a new indicator called NBI ® (Nitrogen Balance Index) which is the ratio Chlorophyll/Flavonoids (related to Nitrogen/Carbon allocation), it is now possible to get an earlier information about the nitrogen status of cultures.
Page 1: Dualex ® 4 scientific FLAVONOid &

DUALEX SCIENTIFIC - Force-A

Create successful ePaper yourself

Delete template?

Save as template?