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STORAGE AND REUSE OF DRAINAGE WATER<br />

INGRID WESSTRÖM 1 , ABRAHAM JOEL 1<br />

1 I. WESSTRÖM, Swedish University of Agricultural Sciences, Department of Soil and Environment, Division of soil and water management,<br />

SwedenIngrid.Wesstrom@mark.slu.se., 1 A. JOEL, Abraham.Joel@mark.slu.se.<br />

CSBE100159 – The effects of drainage water storage in ponds on nutrient leaching and water<br />

resource management were examined in a three-year (2006-2008) field experiment in a 163 km 2<br />

study area in southern Sweden. The land use in the area is mainly intensive agriculture and<br />

approximately 2.5 million m 3 of groundwater are used for irrigation every summer. In 2004, 27<br />

small water storage ponds were constructed in the area. The total storage capacity of these ponds is<br />

355 000 m 3 and if they were to be refilled e.g. 1.5 times per season, the groundwater use for<br />

irrigation could be decreased by 20%. This study examined the effects of the ponds on nutrient<br />

transport and water resource management and developed an index for risk assessment of drainage<br />

water quality. Weather parameters and changes in water storage were recorded in the field and<br />

samples of water entering and leaving the ponds were collected. Analyses of the water revealed<br />

that the ponds acted as a trap for transported nitrogen and phosphorus within the catchment. Digital<br />

data on land use, soil type, drainage network and slope gradients were used to identify watershed<br />

boundaries and to evaluate the impact of watershed properties on water quality. The potential nonpoint<br />

pollution indicator method (PNPPI) developed for assessing catchment potential as a<br />

contributor of nitrogen and phosphorus leaching proved useful. However, the temporal variability<br />

was not fully considered and a procedure for including point sources of pollution should be added.<br />

THE ROLE OF CURLI AND CELLULOSE IN THE TRANSPORT AND SURVIVAL OF<br />

ESCHERICHIA COLI ON A CENTRAL NEW YORK DAIRY FARM<br />

ANTHONY E. SALVUCCI 1 , MARA ELTON 2 , JULIE D. SILER 2 , WEI ZHANG 1 , BRIAN K. RICHARDS 1 , LARRY<br />

D. GEOHRING 1 , LORIN D. WARNICK 2 , ANTHONY G. HAY 3 , TAMMO S. STEENHUIS 1<br />

1 Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA, aes84@cornell.edu, 2 Department of<br />

Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14853, USA, 3 Department of Microbiology, Cornell University,<br />

Ithaca, NY 14853, USA<br />

CSBE100161 – Enterobacterial pathogens such as Escherichia coli are persistently deposited in the<br />

environment through the spreading of manure wastes onto agricultural soils, representing serious<br />

water quality and human health concerns. In this experiment, E. coli isolates were collected from a<br />

dairy farm in Central New York at three distinct locations: (i) cow housing, (ii) calf housing, and<br />

(iii) field drain (tile) effluent. These environmental E. coli isolates were analyzed for the cell<br />

surface components cellulose and curli, traits that have been linked to increased environmental<br />

survival and transport through soil. Our results showed a high amount of diversity amongst E. coli<br />

isolates at each spatial location. Isolates collected from cow housing and calf housing displayed<br />

highly variable curli and cellulose-producing community profiles from one sampling week to<br />

another. However, isolates collected from the drain tile effluent consistently displayed similar curli<br />

and cellulose production communities over all sampling dates. These results indicate that the<br />

subsurface soil and presence of drain tiles tend to select for a certain subset of E. coli strains,<br />

perhaps better adapted for environmental survival and/or transport.<br />

50<br />

XVII th World Congress of the International Commission of Agricultural and Biosystems Engineering (CIGR) – Québec City, Canada – June 13-17, 2010

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