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Triggering an external device in MetaMorph - Molecular Devices

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<strong>Trigger<strong>in</strong>g</strong> <strong>an</strong> <strong>external</strong> <strong>device</strong> through <strong>MetaMorph</strong> ®<br />

software<br />

Abstract<br />

This technical note will describe how to trigger <strong>an</strong> <strong>external</strong><br />

<strong>device</strong> us<strong>in</strong>g the journal<strong>in</strong>g function of <strong>MetaMorph</strong> software.<br />

It will describe us<strong>in</strong>g either a p<strong>in</strong> from a Parallel port (LPT) or a<br />

digital ch<strong>an</strong>nel from a DA board (DAQ or DDA0 series) to send<br />

a 5 volt signal to <strong>an</strong> <strong>external</strong> <strong>device</strong>.<br />

ARTICLE #<br />

T20134<br />

PRODUCTS<br />

<strong>MetaMorph</strong> ®<br />

MetaFluor ®<br />

MetaVue ®<br />

CREATED<br />

22-J<strong>an</strong>uary-2010<br />

LAST UPDATED<br />

02-March-2010<br />

Confirm software option is present<br />

In the Meta Imag<strong>in</strong>g series Adm<strong>in</strong>istrator, confirm that the CUSTOM IO drop<strong>in</strong> is<br />

checked (enabled).<br />

If the drop<strong>in</strong> is not present make sure that you are show<strong>in</strong>g the category Occasional. If<br />

it is still not present you are either us<strong>in</strong>g <strong>an</strong> offl<strong>in</strong>e version of the software or you do not<br />

have the Digital IO option on your software license.<br />

1


TRIGGERING EXTERNAL DEVICES<br />

Confirm a digital output <strong>device</strong> is present<br />

In the Meta Imag<strong>in</strong>g Series Adm<strong>in</strong>istrator make sure that the appropriate digital<br />

<strong>in</strong>put/output <strong>device</strong> is <strong>in</strong>stalled as a system <strong>device</strong> <strong>an</strong>d is part of the hardware sett<strong>in</strong>g. If<br />

you are add<strong>in</strong>g a parallel port make sure that you specify which port you will be us<strong>in</strong>g<br />

<strong>an</strong>d if you w<strong>an</strong>t <strong>an</strong>y p<strong>in</strong>s to start high click. If you w<strong>an</strong>t to rename a p<strong>in</strong> to make<br />

referr<strong>in</strong>g to a trigger easier you c<strong>an</strong> ch<strong>an</strong>ge its label <strong>in</strong> the dialog below:<br />

.<br />

Us<strong>in</strong>g the digital trigger<br />

With<strong>in</strong> <strong>MetaMorph</strong> <strong>in</strong> the <strong>Devices</strong> menu will be a sub-menu Custom IO Control. With<strong>in</strong><br />

the Custom IO Control sub-menu there will be three comm<strong>an</strong>ds referr<strong>in</strong>g to digital I/O<br />

<strong>device</strong>s:<br />

2<br />

©2010 <strong>Molecular</strong> <strong>Devices</strong> (US) Inc. Pr<strong>in</strong>ted <strong>in</strong> the U.S.A.<br />

METAMORPH, META IMAGING SERIES, METAFLUOR <strong>an</strong>d the METAMORPH logo are registered trademarks of <strong>Molecular</strong> <strong>Devices</strong> (US)<br />

Inc.<br />

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.


TRIGGERING EXTERNAL DEVICES<br />

Before do<strong>in</strong>g <strong>an</strong>y digital trigger<strong>in</strong>g you must tell the software which digital <strong>device</strong> you<br />

are referr<strong>in</strong>g to. Use Select Digital IO Device to choose the appropriate <strong>device</strong>:<br />

Once the digital I/O <strong>device</strong> has been chosen you c<strong>an</strong> set the <strong>in</strong>dividual l<strong>in</strong>es on the<br />

<strong>device</strong> us<strong>in</strong>g the Set Digital I/O function. For each l<strong>in</strong>e there are three options: On, Off,<br />

Ignore. If a l<strong>in</strong>e is set to On or Off the l<strong>in</strong>e will be set high or low. If a l<strong>in</strong>e is set to<br />

Ignore the software will not ch<strong>an</strong>ge its sett<strong>in</strong>g whether it is high or low. Cont<strong>in</strong>uous<br />

Update of L<strong>in</strong>e Assignments <strong>in</strong>dicates that the l<strong>in</strong>e ch<strong>an</strong>ges occur automatically<br />

<strong>in</strong>teractively without click<strong>in</strong>g the button.<br />

Given this procedure a typical journal run from a taskbar or with<strong>in</strong> a Multi Dimensional<br />

Acquisition experiment would look like the journal shown below:<br />

3<br />

©2010 <strong>Molecular</strong> <strong>Devices</strong> (US) Inc. Pr<strong>in</strong>ted <strong>in</strong> the U.S.A.<br />

METAMORPH, META IMAGING SERIES, METAFLUOR <strong>an</strong>d the METAMORPH logo are registered trademarks of <strong>Molecular</strong> <strong>Devices</strong> (US)<br />

Inc.<br />

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.


TRIGGERING EXTERNAL DEVICES<br />

Us<strong>in</strong>g the digital triggers dur<strong>in</strong>g stream acquisition<br />

One of the most common uses for trigger<strong>in</strong>g <strong>external</strong> <strong>device</strong>s is dur<strong>in</strong>g Stream<br />

Acquisition. To do this turn on the option Trigger component positions at specific<br />

frames at the bottom of the Acquire tab of Stream Acquisition.<br />

When the Trigger component positions at specific frames option has been enabled<br />

the Trigger tab appears <strong>in</strong> the dialog. Choose whether to send one or more triggers,<br />

<strong>an</strong>d when to send the trigger(s). Choose a Digital IO component <strong>an</strong>d set the<br />

particular p<strong>in</strong>e High or Low. Once this is set click the button to add the trigger to<br />

the stream protocol.<br />

4<br />

©2010 <strong>Molecular</strong> <strong>Devices</strong> (US) Inc. Pr<strong>in</strong>ted <strong>in</strong> the U.S.A.<br />

METAMORPH, META IMAGING SERIES, METAFLUOR <strong>an</strong>d the METAMORPH logo are registered trademarks of <strong>Molecular</strong> <strong>Devices</strong> (US)<br />

Inc.<br />

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.


TRIGGERING EXTERNAL DEVICES<br />

If there are <strong>an</strong>y further issues, please contact Technical support at 800-635-5577 x1820 or<br />

support.dtn@moldev.com.<br />

5<br />

©2010 <strong>Molecular</strong> <strong>Devices</strong> (US) Inc. Pr<strong>in</strong>ted <strong>in</strong> the U.S.A.<br />

METAMORPH, META IMAGING SERIES, METAFLUOR <strong>an</strong>d the METAMORPH logo are registered trademarks of <strong>Molecular</strong> <strong>Devices</strong> (US)<br />

Inc.<br />

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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