OctOplus fluOrescence imager - NH DyeAGNOSTICS

More documents

Recommendations

Info

1D Fluorescence Imaging The OCTOPLUS power LED module with diffuser lenses excites up to 4 different fluorophores. Highly specific excitation and emission filters avoid any crosstalk issues. Figure 1 shows 4 differently fluorescent labeled proteins separated by SDS-PAGE and imaged by OCTOPLUS. Complex samples of less than 1 µg of protein can be analyzed with high accuracy (fig. 2). 1.000 0.500 0.250 0.125 0.062 0.031 0.016 Protein load (μg) BSA (66.0 kDa) + G-Dye200 Casein (24.0 kDa) + T-Rex 330 Lactoglobulin (18.4 kDa) + G-Dye100 RNaseA (13.7 kDa) + T-Red 410 Fig. 1. Serial dilution of BSA, Casein, Lactoglobulin and RNase A labeled with G-Dye and T-Dye fluorophores. Proteins were separated by SDS-PAGE and scanned by OCTOPLUS. Scanning times: G-Dye200: 50 sec, T-Rex 330: 30 sec, G-Dye100: 30 sec, T-Red 410: 90 sec. 5.000 1.000 0.500 0.250 0.125 0.062 Protein load (μg) Fig. 2. T-Green 210 labeled E. coli total protein extract. Separation by SDS-PAGE. OCTOPLUS scanning time: 50 sec.
Signal sensitivity & linearity The OCTOPLUS system detects fluorescent signals from minimally labeled proteins (one fluorophore per protein) in the lower nanogram range. Even in this range, the signal linearity remains at an ideal level (R 2 = 0.996 [fig.3.]). 1.000 0.500 0.250 0.125 0.062 0.031 0.016 0.008 Protein load (μg) Signal intensity (AU) 100 80 60 R² = 0.996 40 20 0 0.0 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.0 Amount of protein (µg) Fig. 3. Serial dilution of BSA minimally labeled with T-Rex 330. Proteins were separated by SDS-PAGE and scanned with OCTOPLUS. Signal intensities were analyzed by LabImage 1D L340 (Kapelan GmbH, Germany). Related products T-Dye fast & easy protein labeling kits
Page 1 and 2: OCTOPLUS FLUORESCENCE IMAGER for po
Page 6 and 7: 2D Fluorescence imaging The OCTOPLU
Page 8 and 9: Chemiluminescence With a superior C
Page 10 and 11: Octoplus inside Superior optic and
Page 12: Ordering information PR 130 OCTOPLU

OctOplus fluOrescence imager - NH DyeAGNOSTICS

Create successful ePaper yourself

Delete template?

Save as template?