OctOplus fluOrescence imager - NH DyeAGNOSTICS

OCTOPLUS
FLUORESCENCE IMAGER
for powerful & fast 2D image acquisition
The gold standard for CCD-based RGB fluorescence detection
Approved for

Octoplus fluorescence imager
For powerful & fast 2D image acquisition
Accurately analyzing fluorescent labeled proteins requires a high performing imaging system.
The new OCTOPLUS FLUORESCENCE IMAGER has been specifically developed for experiments
with powerful Refraction-2D and Saturn-2D technologies.
The system combines the fast image acquisition of fluorescent gels and blots with high sensitivity
and robust setup required for daily routine. Thereby, the OCTOPLUS FLUORESCENCE
IMAGER sets a unique standard for fast fluorescence multiplex 2D imaging as well as ECL,
and optionally for silver- or Coomassie® blue stained gels.
Simultaneous fluorescence imaging of up to 4 different dyes
Sensitive & quantitative chemiluminescence
Bright colorimetric imaging
Made in Germany

1D Fluorescence Imaging
The OCTOPLUS power LED module with diffuser lenses excites up to 4 different fluorophores.
Highly specific excitation and emission filters avoid any crosstalk issues. Figure 1
shows 4 differently fluorescent labeled proteins separated by SDS-PAGE and imaged by
OCTOPLUS. Complex samples of less than 1 µg of protein can be analyzed with high
accuracy (fig. 2).
1.000 0.500 0.250 0.125 0.062 0.031 0.016
Protein load (μg)
BSA (66.0 kDa)
+ G-Dye200
Casein (24.0 kDa)
+ T-Rex 330
Lactoglobulin (18.4 kDa)
+ G-Dye100
RNaseA (13.7 kDa)
+ T-Red 410
Fig. 1. Serial dilution of BSA, Casein, Lactoglobulin and RNase A labeled with G-Dye and T-Dye
fluorophores. Proteins were separated by SDS-PAGE and scanned by OCTOPLUS. Scanning times:
G-Dye200: 50 sec, T-Rex 330: 30 sec, G-Dye100: 30 sec, T-Red 410: 90 sec.
5.000 1.000 0.500 0.250 0.125 0.062 Protein load (μg)
Fig. 2. T-Green 210 labeled E. coli total protein extract. Separation by
SDS-PAGE. OCTOPLUS scanning time: 50 sec.

Signal sensitivity & linearity
The OCTOPLUS system detects fluorescent signals from minimally labeled proteins (one
fluorophore per protein) in the lower nanogram range. Even in this range, the signal
linearity remains at an ideal level (R 2 = 0.996 [fig.3.]).
1.000 0.500 0.250 0.125 0.062 0.031 0.016 0.008
Protein load (μg)
Signal intensity (AU)
100
80
60
R² = 0.996
40
20
0
0.0 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.0
Amount of protein (µg)
Fig. 3. Serial dilution of BSA minimally labeled with T-Rex 330. Proteins were separated by
SDS-PAGE and scanned with OCTOPLUS. Signal intensities were analyzed by LabImage 1D L340
(Kapelan GmbH, Germany).
Related
products
T-Dye
fast & easy
protein
labeling kits

2D Fluorescence imaging
The OCTOPLUS FLUORESCENCE IMAGER is especially designed for Refraction-2D and
Saturn-2D multiplex fluorescence 2D gel analysis. The powerful combination of carefully
developed system components for fluorescence light excitation and detection create a
perfect system for sensitive image acquisition.
Scanning time for a single Refraction-2D gel (images for G-Dye100, G-Dye200 and
G-Dye300) is performed within minutes. A complete series of gels (e.g. six Refraction-2D
gels) can be scanned in less than one hour.
5 min
total image
acquisition time
Refraction-2D analysis of two different Arabidopsis thaliana ecotypes.
Related
products
Refraction-2D
labeling kit
for powerful
multiplexing
Saturn-2D
labeling kit
for scarce
samples

OCTOPLUS Fluorescence Imager
Blue LED, G100 filter
Bit depth: 16 bit
Pixel size: 7 µm @ 55,000 full well capacity
Gel size: 24 x 20 cm, pH 3-10
Sample: 50 µg total protein from E. coli
Fluorescent label: G-Dye100
Scanning time: 20 sec
OCTOPLUS Fluorescence Imager
Green LED, G200 filter
Bit depth: 16 bit
Pixel size: 7 µm @ 55,000 full well capacity
Gel size: 24 x 20 cm, pH 3-10
Sample: 50 µg total protein from E. coli
Fluorescent label: G-Dye200
Scanning time: 50 sec
OCTOPLUS Fluorescence Imager
Red LED, G300 filter
Bit depth: 16 bit
Pixel size: 7 µm @ 55,000 full well capacity
Gel size: 24 x 20 cm, pH 3-10
Sample: 50 µg total protein from E. coli
Fluorescent label: G-Dye300
Scanning time: 20 sec

Chemiluminescence
With a superior CCD chip, a lab quality lens and a 16 bit dynamic range (4.7 orders of
magnitude) OCTOPLUS captures chemiluminescence at the highest resolution available on
the market.
1000 500 250 125 62.5 31.25 16 8
Protein load (pg)
Fig. 4. Serial dilution of Casein. Proteins were separated by SDS-PAGE, then transfered by
Western blotting onto a nitrocellulose membrane. The blot was subjected to a Casein antibody
and then to a HRP-conjugated secondary antibody. The proteins were detected by ECL (Pierce)
with an exposure time of 2.5 min.
Colorimetric applications (optional)
Using the optional white transillumination module, the perfect imaging of silver or
Coomassie® blue stained gels can be achieved.
Fig. 5a and 5b. Colorimetric analysis of Coomassie® blue stained 2D and 1D gels.

Octoplus fluorescence imager
Image capture software
The image acquisition is performed by the easy-to-use OCTOPLUS image capture software.
Your raw image data are saved and a copy is taken for further analysis by 1D and 2D
software. This data separation allows you to always revert back to your original images.
Related
products
2DXPLORER
labeling kits and
analysis software
per 2D gel

Octoplus inside
Superior optic and
CCD camera system
Fast remote focus
Adjustable power
LED sample tray
Robust housing
for daily routine

Instrument specifications
CCD Kamera
Kodak CCD full frame chip with mircolens technology
Cooling
4-stage Peltier cooling (delta T -60°C)
Chip resolution
3.2 MP
Pixel size Approx. 7 µm, full well capacity 55,000 e-
Dynamic range
16-bit (65,536 grey levels), 4.7 orders of magnitude
Lens
Schneider-Kreuznach (F: 0.95 / 25 mm)
Focusing
Manual remote operation
Binning modes 1 x 1, 2 x 2, 3 x 3, ... , 10 x 10
Fluorescence unit
Power LED module for RGB fluorescence detection,
upgradable to IR fluorescence detection
Filter
Highly specific blue, red & green filter (standard), highly
specific IR filter optional, upgradable to 3 additional filters
Sample size
22 x 28 cm
Interface
USB 2.0 / Ethernet
Temperature Up to 30°C
Size (W x H x D)
41 cm x 90 cm x 40 cm
Weight
Approx. 45 kg
Supply voltage
100 - 240 V
Frequency
50 / 60 Hz

Ordering information
PR 130 OCTOPLUS Fluorescence Imager
• Power LED module for highly specific blue, green & red fluorescence
• Chemiluminescence
• Image capture and 1D analysis software
PR131 Highly specific far red power LED module
PR132 White light transmission module
PR133 Analysis PC with high resolution display (21.5”)
PR136 2D analysis software Delta2D
Related products
PR03
PR04
Low fluorescent glass cassettes, size 8 x 10 cm
Low fluorescent glass cassettes, size 22 x 27.5 cm
Contact
NH DyeAGNOSTICS GmbH
Weinbergweg 23
D-06120 Halle
Germany
Fon: +49 (0) 345-2799 6413
Fax: +49 (0) 345-2799 6412
E-Mail: info@dyeagnostics.com
sales@dyeagnostics.com
www.dyeagnostics.com
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