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Article 80
Complete reductive dechlorination of tetrachloroethene
to ethene by anaerobic microbial enrichment
culture developed from sediment
Biotechnol Lett. 2010 Dec; 32(12):1829-35.
Kim BH, Baek KH, Cho DH, Sung Y, Koh SC, Ahn CY,
Oh HM, Kim HS *
* Correspondence: hkim@kribb.re.kr
Environmental Biotechnology Research Center
A mixed, anaerobic microbial enrichment culture, AMEC-4P,
was developed that uses lactate as the electron donor for
the reductive dechlorination of tetrachloroethene (PCE) to
ethene. AMEC-4P consistently and completely converted
2 μM PCE to cis-1,2-dichloroethene ( cis-DCE) within 13
days, and the intermediate, cis-DCE, was then completely
dechlorinated to ethene after 130 days. Dechlorination rates
for PCE to cis-DCE, cis-DCE to VC, and VC to ethene
were 243, 27, and 41
μmol/l/day, respectively.
Geobacter
lovleyi and a Dehalococcoides sp. were identified from their
16S rRNA sequences to be the dominant phylotypes in
AMEC-4P.
PMID: 20714784
Keywords: Anaerobic microbial enrichment; Anaerobiosis;
Bacteria; Chlorine; Dechlorination; Microbial
community analysis; Oxidation-reduction;
Tetrachloroethene
Article 81
Classification of rice (Oryza sativa L. Japonica
nipponbare) immunophilins (FKBPs, CYPs) and
expression patterns under water stress
BMC Plant Biol. 2010 Nov; 10:253.
Ahn JC, Kim DW, You YN, Seok MS, Park JM, Hwang
H, Kim BG, Luan S, Park HS * , Cho HS *
* Correspondence: hspark@kribb.re.kr hscho@kribb.re.kr
Genome Resource Center
Plant Systems Engineering Research Center
BACKGROUND: FK506 binding proteins (FKBPs) and cyclophilins
(CYPs) are abundant and ubiquitous proteins belonging
to the peptidyl-prolyl cis/trans isomerase (PPIase)
superfamily, which regulate much of metabolism through
a chaperone or an isomerization of proline residues during
protein folding. They are collectively referred to as immunophilin
(IMM), being present in almost all cellular
organs. In particular, a number of IMMs relate to environmental
stresses.
RESULTS: FKBP and CYP proteins in rice (Oryza sativa
cv. Japonica) were identified and classified, and given the
appropriate name for each IMM, considering the ortholog-relation
with Arabidopsis and Chlamydomonas or molecular
weight of the proteins. 29 FKBP and 27 CYP genes
can putatively be identified in rice; among them, a number
of genes can be putatively classified as orthologs of
Arabidopsis IMMs. However, some genes were novel, did
not match with those of Arabidopsis and Chlamydomonas,
and several genes were paralogs by genetic duplication.
Among 56 IMMs in rice, a significant number are regulated
by salt and/or desiccation stress. In addition, their expression
levels responding to the water-stress have been analyzed
in different tissues, and some subcellular IMMs located by
means of tagging with GFP protein.
CONCLUSION: Like other green photosynthetic organisms
such as Arabidopsis (23 FKBPs and 29 CYPs) and
Chlamydomonas (23 FKBs and 26 CYNs), rice has the highest
number of IMM genes among organisms reported so
far, suggesting that the numbers relate closely to
photosynthesis. Classification of the putative FKBPs and
CYPs in rice provides the information about their evolutional/functional
significance when comparisons are drawn
with the relatively well studied genera, Arabidopsis and
Chlamydomonas. In addition, many of the genes upregulated
by water stress offer the possibility of manipulating the stress
responses in rice.
PMID: 21087465
Keywords: Cell nucleus; Cyclophilins; Cytoplasm;
Immunophilins; Microscopy, fluorescence; Oryza
sativa; Phylogeny; Protein isoforms; Sodium
chloride; Tacrolimus binding proteins; Tobacco
2010 KRIBB Article Abstracts | 47 |