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M.F.Sc & <strong>PhD</strong> Programs<br />

in <strong>Fish</strong> <strong>Processing</strong><br />

<strong>Technology</strong> - <strong>Syllabus</strong><br />

<strong>Central</strong> <strong>Institute</strong> of <strong>Fish</strong>eries Education<br />

Mumbai<br />

18/4/08 1


M.F.Sc. (<strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong>) Major Courses<br />

Code Course Title Credits<br />

CORE COURSES<br />

1. FPT 501 <strong>Technology</strong> of fish freezing and frozen storage 1+1<br />

2. FPT 502 Thermal <strong>Processing</strong> fishery products 1+1<br />

3. FPT 503 Quality assurance, management and certification 1+1<br />

4. FPT 504 Applied <strong>Fish</strong> Biochemistry 1+1<br />

5. FPT 505 Techniques in Microbiology 1+1<br />

6. FPT 506 Cured, dehydrated and smoked fishery products 1+1<br />

OPTIONAL COURSES<br />

1. FPT 507 Handling, storage and transport of fresh fish 1+1<br />

2. FPT 508 <strong>Technology</strong> of mince based fish products 1+1<br />

3. FPT 509 Additives in <strong>Fish</strong> <strong>Processing</strong> 1+1<br />

4. FPT 510 <strong>Fish</strong>ing technology 2+1<br />

5. FPT 511 Research and Statistical methods 2+1<br />

6. FPT 512 <strong>Fish</strong> by-products, utilization of fishery wastes 1+1<br />

7. FPT 513 Microorganisms of public health significance 1+1<br />

8. FPT 514 Instrumentation in fish quality assessment 1+1<br />

9. FPT 515 Packaging of fish and fishery product 1+1<br />

<strong>PhD</strong> (<strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong>) Major Courses<br />

Code Course Title Credits<br />

CORE COURSES<br />

1. FPT 701 Biochemical Techniques in <strong>Fish</strong> Analysis 2+1<br />

2. FPT 702 Functional Properties of Proteins from <strong>Fish</strong> and<br />

2+1<br />

Shellfish<br />

3. FPT 703 Quality Management Systems 2+1<br />

OPTIONAL COURSES<br />

1. FPT 707 Lipids of Aquatic Origin 2+1<br />

2. FPT 708 Microbial Hazards in <strong>Fish</strong> <strong>Processing</strong> 2+1<br />

3. FPT 709 Vitamins, minerals, flavour bearing components in 2+1<br />

aquatic organisms<br />

4. FPT 711 Toxins and Contaminants 2+1<br />

5. FPT 712 Nutritional Aspects and Nutrition Labeling 2+1<br />

6. FPT 713 Environmental Impact of <strong>Fish</strong>ery Industries 2+1<br />

7. FPT 714 Specialty products and Value Added products 2+1<br />

18/4/08 2


<strong>MFSc</strong> <strong>Syllabus</strong><br />

Core Courses<br />

<strong>Technology</strong> of fish freezing and frozen storage 1 + 1<br />

Objective:<br />

• To teach students about various aspects of freezing of fish<br />

• To teach chemical, bacterial and sensory changes during freezing<br />

Theory:<br />

Freezing: Structure of water and ice, Influence of solutes on the structure of water and ice,<br />

phase equlibria and freezing curves of pure water and binary solutions, freezing curves for<br />

fish, determination of freezing points from time- temperature plots, calculation of freezing<br />

time, crystallization, nucleation, homogeneous and heterogeneous nucleation, super<br />

cooling, crystal growth, eutectic point, location of ice crystals in tissue, changes during<br />

freezing.<br />

Technological aspects of freezing: Methods of freezing, comparison of various freezing<br />

methods, selection of a freezing method, product processing and packaging, chemical<br />

treatment prior to freezing, antioxidants, cryoprotectants and other additives, theories of<br />

cryoprotection, glazing.<br />

Frozen storage: Physical changes - freezer burn and recrystallisation, different types of<br />

recrystallisation.<br />

Chemical changes in lipids, proteins and nucleotides, freeze denaturation and theories on<br />

denaturation, changes in pH, bacterial changes, sensory changes, texture, taste, odour,<br />

effect of post-mortem condition on sensory qualities.<br />

Water holding capacity, time temperature tolerance, temperature and duration of storage on<br />

quality and shelf life. Arrangements within a cold storage, handling and stacking systems,<br />

space requirement, precautions to reduce temperature increase in a cold storage.<br />

18/4/08 3


Filleting of fish, treatments, glazing, packaging and freezing. <strong>Processing</strong> of prawns, lobster,<br />

squid, cuttle fish, crab etc. for freezing.<br />

Practical:<br />

Filleting of fish, treatments, glazing, packaging, freezing,<br />

<strong>Processing</strong> of Prawns, Lobster, Squid, Cuttle <strong>Fish</strong>, Crab etc. in different styles, Packaging<br />

and Freezing,<br />

Freezing curve, determination of freezing point.<br />

Studies on physical, chemical and sensory changes.<br />

Reference:<br />

• Advances in <strong>Fish</strong> processing <strong>Technology</strong>, Sen D. P., Pub. Allied Publishers Pvt. Ltd.<br />

New Delhi (2005).<br />

• <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong> (1992) G. M. Hull. (Ed), Blackie Academic and<br />

Professional, London<br />

• Food Freezing Today,and Tomorrow, Springer - Verlage, London<br />

• Food Refrigeration Processes (1990) By Cleland C Andrew, Elsevier Applied<br />

Sciences, London<br />

• Freezing Effects on Food Quality, Marcel Dekker, Inc. New York<br />

• Govindan, T. K., <strong>Fish</strong> processing <strong>Technology</strong>, Pub. Oxford & IBH Publishing Co.,<br />

New Delhi (1985).<br />

• Low Temperature Preservation of Foods and Living Matter Marcel Dekker, Inc. New<br />

York<br />

• Postharvest <strong>Technology</strong> of fish and fish products. (2001). Balachandran, K. K., Pub.<br />

Daya Publishing House, Delhi,<br />

• Principle of Food Science. Fennema, O. R. (editor), Publ. Marcel Dekker Inc.<br />

NewYark<br />

18/4/08 4


• Principles of Total Quality. (1994) St. Leuie Press, USA.<br />

• Quality and Stability of Frozen Foods, Wiley Intersciece (John Wiley and Sons) New<br />

York<br />

• Text Book of <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong>; Gopakumar K. (editor), Pub. ICAR, New<br />

Delhi (2002)<br />

Thermal processing of fish products 1 +1<br />

Objective:<br />

• To teach the students various aspects of thermal / heat processing<br />

• To teach irradiation technology to enhance shelf-life of fish and its effect on fish<br />

quality<br />

• To teach various types of packaging techniques and material<br />

Theory:<br />

Principles of thermal processing. Mechanism of heat transfer: conduction, convection,<br />

radiation and dielectric and microwave heating, unsteady state of transfer, heat resistance<br />

of bacteria and spores, decimal reduction time, thermal death time, "Z" and "F" values, heat<br />

penetration, cold point, can size, shape, contents etc. on heat penetration, determination of<br />

process time. Significance of thermal death curve, graphical, formula, nomogram methods –<br />

F 0 value, cook value, D value, integrated F value and their inter-relationship.<br />

Heat processing and heating equipment,<br />

Classification of foods: low acid, medium acid and acidic foods, absolute sterility, statistical<br />

sterility, commercial sterility, pasteurisation and sterilisation.<br />

Canning process, steps involved, process flow, additives, HTST processing and aseptic<br />

canning, principles and process details, canning machinery and equipment, canning<br />

process for fish/shellfish, value added canned products.<br />

18/4/08 5


Spoilage of canned food, physical, chemical and microbial, Thermobacteriology, death of<br />

bacteria, autosterilisation bacteriology of canned/heat processed fishery products,<br />

examination of cans and seams.<br />

Canning plant location: Practical considerations, canning plant facilities, layout design.<br />

Flexible packing, retort pouch processing of fish and fishery products principles and<br />

techniques. Combination and synergistic effects.<br />

Hurdle technology: Combination with heat, heat and hydrostatic pressure, heat and low pH,<br />

heat and NaCI and nitrite, combination with ionising radiation, irradiation and hydrostatic<br />

pressure, irradiation and NaCI, irradiation and other adjuncts, heat and irradiation,<br />

irradiation and low temperature, low pH and specific acids, low a w and adjuncts.<br />

Irradiation: Radiation sources, units, dose levels radappertization, radicidation, radurization,<br />

effects of irradiation on protein, lipids, vitamins, bacteriological aspects, physical properties,<br />

shelf life.<br />

Practical:<br />

Evaluation of pasteurisation and sterilisation, determination of TDT and F value Examination<br />

of canned foods, can seams, testing sterility, isolation of Bacillus and Clostridium Spp, spore<br />

staining, heat penetration curve and cooling curve, canning operations for different<br />

fish/shellfish products<br />

Double seam profile, Heat Penetration Curve, F 0 Value, Z value, Process time, Canning of<br />

table fishes, Bivavles, Crustaceans in different containers, Operation of over pressure<br />

autoclaves, Canned culinary preparations, Examination of canned fishery products.<br />

Reference:<br />

• A Handbook of Food Packaging (1983) Leonard Hill, London<br />

• Advances in <strong>Fish</strong> processing <strong>Technology</strong>. (2005). Sen D. P., Pub. Allied Publishers<br />

Pvt. Ltd. New Delhi.<br />

• Canned Foods. (1980) Hersom and Hulland, Churchill Livingstone, New York<br />

18/4/08 6


• <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong> (1992) G. M. Hull. (Ed), Blackie Academic and<br />

Professional, London<br />

• Food Canning <strong>Technology</strong> (1997) Larousse and Brown Eds., Willey VCH New York<br />

• Food Irradiation (1991) Thorne, S (Ed), Elsevier Applied Science, London<br />

• Food Packaging – Principles and Practices (1993) Marcel Dekker, Inc. New York<br />

• Govindan, T. K., <strong>Fish</strong> processing <strong>Technology</strong>, Pub. Oxford & IBH Publishing Co.,<br />

New Delhi (1985).<br />

• HACCP in meat, poultry and fish processing (1992). Garret, E.S., Hudak Roos, M<br />

and Ward, D.R Advances in meat research series. Vol 1 O.<br />

• Handbook of Food Engineering Practices (1997) Valentus, K.J.,Rotskin, E and Paul<br />

Singh, R CRC press New York<br />

• Heat Transfer and Food Products (1988) Elsevier Applied Science, London<br />

• Introduction to Food engineering (2001) Paul Singh, R, Dennis R Heldman,<br />

Academic Press, London<br />

• Mechanism of Action of Food Proservation Procedures (1989) W. Gould (Ed),<br />

Elsevier Applied Science<br />

• Manual on <strong>Fish</strong> Canning. (1988). Warne, D., FAD <strong>Fish</strong>eries Technical paper 285<br />

• NFPA Laboratory Manual of Food Canners and Processors (1980) AVI. Publishing<br />

Co. Westport, Connecticut<br />

• Novel Food Packaging Tech-niques-(2003} ~f9ress-New~York<br />

• Physical Principles of Food Preservation, (1975) Marcel Dekker Inc. New York<br />

• Postharvest <strong>Technology</strong> of fish and fish products. (2001). Balachandran, K. K., Pub.<br />

Daya Publishing House, Delhi,<br />

18/4/08 7


• Principle of Food Science. Fennema, O. R. (editor), Publ. Marcel Dekker Inc.<br />

NewYark<br />

• Seafood Effect of Technolongy on Nutrition (1990) Pegott, G.M. and Tucker, B.W.<br />

(Eds) Marcel Dekker I nc. New York<br />

• Text Book of <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong>; Gopakumar K. (editor), Pub. ICAR, New<br />

Delhi (2002)<br />

• Thermo Bacteriology in Food <strong>Processing</strong> (1973). Stumbo, C.R ,Academic Press,<br />

New York<br />

Quality assurance, management and certification 1 + 1<br />

Objective:<br />

• To teach various aspects of quality assurance system, quality management and<br />

national / international certification system<br />

• To teach factory sanitation and hygiene, water quality and standards<br />

• To teach quality affecting parameters<br />

Theory:<br />

Quality management, total quality concept and application in fish trade. Quality assessment<br />

of fish and fishery products - physical, chemical, organoleptic and microbiological quality<br />

standards.<br />

Inspection and quality assurance: <strong>Fish</strong> inspection in India, process water quality in fishery<br />

industry, product quality, water analysis, treatments, chlorination, ozonisation, UV radiation,<br />

reverse osmosis, techniques to remove pesticides and heavy metals. Sensory evaluation of<br />

fish and fish products, basic aspects, different methods of evaluation, taste panel selection<br />

and constitution, statistical analysis.<br />

Quality problem in fishery products: Good manufacturing practices. HACCP and ISO 9000:<br />

2000 series of quality assurance system, validation and audit. National and international<br />

standards, Codex alimentarius, USFDA and EU regulations for fish export trade, IDP<br />

18/4/08 8


and SAT formations in certification of export worthiness of fish processing units, regulations<br />

for fishing vessels, pre-processing and processing plants, EU regulations. ISO22000:2006<br />

Factory sanitation and hygiene: National and international requirements, SSOP<br />

Water quality and standards, water analysis, purification and treatment of water.<br />

Food laws in India, integrated food law.<br />

Practical:<br />

Evaluation of fish / fishery products for organoleptic, chemical and microbial quality.<br />

Methods for analysis for bacterial quality parameters, chemical parameters and filth.<br />

Evaluation of sanitary conditions in fish processing units.<br />

Analysis of typical hazards. Study of correction and corrective action.<br />

SQC : Introduction, statistical principles involved, process control, control charts, variable<br />

and attribute control charts, Acceptance sampling, basic ideas, sampling by attributes single<br />

and double sampling plants, Basic concepts of decision making.<br />

Familiarization with water quality analysis<br />

Reference:<br />

• Advances in <strong>Fish</strong> processing <strong>Technology</strong>, Sen D. P., Pub. Allied Publishers Pvt. Ltd.<br />

New Delhi (2005).<br />

• Assessment and Management of Seafood Safety and Quality. (2003) Free amino<br />

acids Technical Paper No. 444<br />

• <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong> (1992) G. M. Hull. (Ed), Blackie Academic and<br />

Professional, London<br />

• Food borne Disease Handbook. IInd Edn. (2001) Vol.4:Seafood and Environmental<br />

Toxins, Marcel Dekker Inc New York<br />

18/4/08 9


• Handbook of Natural toxins - Vol 3. Marine Toxins and Venom. 1988. Marcel Dekker<br />

Inc. New York<br />

• Handbook of Natural toxins - Vol 4. Bacterial Toxins. 1988. Marcel Dekker Inc. New<br />

York.<br />

• ISO- 9000-3 1995 Springer<br />

• Low Temperature Preservation of Foods and Living Matter Marcel Dekker, Inc. New<br />

York<br />

• Postharvest <strong>Technology</strong> of fish and fish products. (2001). By Balachandran, K. K.,<br />

Pub. Daya Publishing House, Delhi,<br />

• TQM - in New Product Manufacturing. (1992), McGraw Hill Inc. New York 4.<br />

Introduction of Total Quality. (1994) Prentice Hall Inc., USA<br />

• Principles of Total Quality. (1994) St. Leuie Press, USA.<br />

• Seafood toxins 1984. American Chemical Society, Washington, D.C.<br />

• Text Book of <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong>; Gopakumar K. (editor), Pub. ICAR, New<br />

Delhi (2002)<br />

Applied <strong>Fish</strong> Biochemistry (1+1)<br />

Objective:<br />

• To teach macro and trace constituents and nutritive value of fish<br />

• To teach toxins and toxic substances and their toxic effects<br />

• To teach various experimental techniques used in food analysis<br />

Theory:<br />

Seafood proteins:<br />

Sarcoplasmic proteins: Classification, enzymes, hydrolases.<br />

18/4/08 10


Oxidoreductases; Other enzymes, pigments, Heme proteins, Myoglobin, Hemocyanins,<br />

parvalbumins, antifreeze proteins.<br />

Myofibrillar proteins: Myosin – isolation, sub-unit composition, stability, gelation, actin,<br />

allergins, tropomyosin, troponins, paramyosin, connectin. Collagen in fish muscle and skin:<br />

location, connective tissue in the muscle, characteristics of seafood collagen, collagen on<br />

the quality of seafoods, gaping.<br />

Functional properties of seafood proteins: Solubility, emulsification, viscosity, water holding,<br />

gelation, texture profile analysis.<br />

Denaturation: At high and low temperatures and kinetics, dissociation / aggregation /<br />

coagulation etc. reversibility, significance to processing and quality.<br />

Hydrolysis and hydrolysates: Process and applications, proteinases.<br />

Non-protein nitrogenous compounds: Free amino acids, peptides.<br />

Nucleotides, Guanidins, urea, quarternary ammonium compounds etc. TMAO and its<br />

decomposition products, demethylase.<br />

Seafood lipids:<br />

Composition and nutritive value, lipid types and their variations, fatty acid composition of fish<br />

liver and body oils, lipid fractionation, triglycerides, phospholipids, non-saponifiables<br />

includinding sterols and vitamins. Polyunsaturated fatty acids, prostaglandins, beneficial<br />

effects on human health, estimation of lipid fractions, auto-oxidation of fatty acids, pro- and<br />

anti-oxidants, oxidation indices, lipid-protein interactions, oxidized lipids-protein interactions<br />

and their impact on quality, rancidity, lipasas and phospholipases.<br />

Macro and trace elements in fish and shellfish; Minerals of nutritional significance, toxic<br />

metals and their harmful effects and metallothionines.<br />

Flavour bearing compounds in fish.<br />

Nucleotides: Post mortem degradation, K-value<br />

Free amino acids, amines, volatile fatty acids, carbonyls, sulphur containing compounds,<br />

carotenoids, isoprenoids.<br />

Biogenic amines, Aflatoxins in cured fish.<br />

Practical:<br />

Molarity, normality, acid-base, redox titration, buffers<br />

Lipids –Fractionation by TLC and other chromatographic techniques<br />

Fatty acid composition by GLC, Amino acid analysis by HPLC<br />

18/4/08 11


Protein purification methods: (NH4)2SO4/solvent precipitation<br />

Ultracentrifugaion, dialysis & ultrafiltration, gel filtration, electrophoresis, PAGE and SDS-<br />

PAGE,<br />

Marine polysaccharides for food use, molecular biology techniques in fish and bacterial<br />

identification, and topical subjects.<br />

Reference:<br />

• Food Analysis Theory and Practice (1994). Pomeranz, Y. and Meloan, C. E., Avi<br />

Publishing Co. Wert port<br />

• Food Protein Chemistry (1984). By Regenstein, J. M. and Regenstein C. E., Academic<br />

Press INC.<br />

• Food Texicology (part A) and Principal & concepts (partB) (1988). By Concon, J.<br />

Marcel Dekker INC, New York<br />

• Instrumental Methods in Food Analysis (1997) By Pare J.R.J. and Belanger J.M.R.<br />

Elsevier, Netherlands<br />

• Morden Methods of Food Analysis (1984). By Stewart K.K. (Ed). Avi Publishing co.<br />

westport<br />

• Mordern Techniques of Testing <strong>Fish</strong> & <strong>Fish</strong>ers Product for Food & Feed (1967). By<br />

Mukundan M.K. Biochemistry & Nutrition & microbiology division<br />

• Nutrition In approach (1967). By Pike R. L. and Brown M.L. Wilcy Eastern Private<br />

Techniques in Microbiology 1+1<br />

Safety in microbiology laboratory – Prevention of contamination, aerosol sampling,<br />

disinfection and evaluation of disinfectants.<br />

Microscopy – bright-field, fluorescence, phase-contrast, dark ground and electron<br />

microscope.<br />

Staining techniques - chemistry and various types.<br />

18/4/08 12


Sterilisation – principles and various physical and chemical methods.<br />

requirements of microorganisms – growth media and different types.<br />

Nutritional<br />

Isolation, enumeration, preservation and maintenance of cultures - growth curve, different<br />

types of cultures, population estimation techniques.<br />

Routine tests for identification of bacteria - morphological, cultural, biochemical and<br />

serological. Anaerobic bacteria - methods of anerobiosis. Basics of mycological and<br />

virological techniques. Introduction to molecular techniques in Microbiology.<br />

Practicals: Microscopic techniques, isolation, enumeration and identification of<br />

microorganisms, serological techniques, anaerobic bacteria, mycological, virological<br />

and molecular techniques.<br />

Cured, dehydrated, smoked fishery products 1 + 1<br />

Objective:<br />

• To teach about various scientific preservation techniques of fish.<br />

• To teach changes during storage of products<br />

Theory:<br />

Free and bound water in foods, water activity and sorption behaviours of foods, storage<br />

characteristics, microbial spoilage, effects of water activity on chemical deterioration,<br />

enzymatic reaction, non-enzymatic browning, lipid oxidation, reaction between lipids and<br />

proteins, dry fish, control of micro-organisms.<br />

Principles of drying and dehydration: Psychometrics, drying calculation, constant rate and<br />

falling rate, drying time in air, moisture transport mechanism, natural drying, solar drying and<br />

mechanical drying. Different types of dryers: tunnel drier, vacuum drier, drum drier, solar<br />

drier etc.<br />

18/4/08 13


Dehydration of fish products: dehydration ratio, precautions to be taken in fish drying;<br />

denaturation of fish protein<br />

Cured fish, use of salt, factors affecting salt uptake by fish, lean and fatty fish, whole, gutted<br />

or split open, type and size of salt crystals, source of salts and impurities in salts, effect of<br />

impurities on salt penetration, temperature of salting.<br />

Spoilage of dried / cured fish, physical, chemical and microbiological changes, methods to<br />

prevent / control spoilage, extension of shelf life<br />

Smoke curing, chemistry of smoke, composition and properties, smoking methods: cold and<br />

hot method, use of smoke liquids, production of smoke, type of wood used, methods of<br />

smoke generation, carcinogens in smoke, smoke kilns<br />

Marinades: Principles; processing of cold, cooked and fried marinades, shelf life and<br />

spoilage.<br />

<strong>Fish</strong> and shellfish pickles: production, shelf life<br />

Packaging requirements for dry, cured and fermented products.<br />

Practical:<br />

Preparation of dried, cured and fermented fish products, examination of salt, protein,<br />

moisture in dried / cured products, examination of spoilage of dried / cured fish products,<br />

marinades, pickles, sauce,<br />

Reference:<br />

• Advances in <strong>Fish</strong> processing <strong>Technology</strong>, By Sen D. P., Pub. Allied Publishers Pvt.<br />

Ltd. New Delhi (2005).<br />

• Batters and Breadings in Food <strong>Processing</strong> Karel Kulp and Robert<br />

Loewe(Eds),American Association of Cereal Chemists, Inc.St.Paul,Minnesota 55121-<br />

2097 USA.<br />

• Batter and Breading <strong>Technology</strong>, Darrel R. Sudermanand E. Cunningham(Eds), Ellis<br />

Horwood Limited, Publishers, Chichester, England.<br />

18/4/08 14


• <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong> (1992) G. M. Hull. (Ed), Blackie Academic and<br />

Professional, London<br />

• Heat Transfer and Food Products (1988) Elsevier Applied Science, London<br />

• Introduction to Food engineering (2001) Paul Singh, R, Dennis R Heldman,<br />

Academic Press, London<br />

• Manual of processing, packaging and preservation of value added fishery products,<br />

Infofish publication, Infofish, P.O. Box 10893, Kualalampur ,Malaysia<br />

• Natural Antimicrobial Systems and Food Preservation (1994), CAB International<br />

Walligford<br />

• Postharvest <strong>Technology</strong> of fish and fish products. (2001). By Balachandran, K. K.,<br />

Pub. Daya Publishing House, Delhi,<br />

• Principle of Food Science. Fennema, O. R. (editor), Publ. Marcel Dekker Inc.<br />

NewYark<br />

• <strong>Processing</strong> Aquatic Food Products, By Wheaton and Lawson (1985) John Wiley and<br />

Sons New York.<br />

• Text Book of <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong>; Gopakumar K. (editor), Pub. ICAR, New<br />

Delhi (2002)<br />

OPTIONAL COURSES<br />

<strong>Fish</strong> by-products and utilization of fishery wastes 1 + 1<br />

Objective:<br />

• To teach students regarding various fish by-products, and utilization of fishery wastes<br />

Theory:<br />

<strong>Fish</strong> meal: Production - dry and wet process, machinery, control of quality of products,<br />

specifications, packaging and storage<br />

<strong>Fish</strong> body and liver oils: Extraction, purification, preservation and storage, industrial and<br />

nutritional applications of fish oils. Vitamin A & D<br />

18/4/08 15


Essential fatty acid functions of fish oils, poly unsaturated fatty acid (PUFA), production of<br />

concentrates of polyunsaturated fatty acids, preparation of fatty alcohol and amides,<br />

Utilisation of shark: <strong>Processing</strong> of shark meat, removal of urea in meat, filleting, curing and<br />

dehydration, extraction of shark liver oil, Vitamin A, D, squalene, ambergris, curing and<br />

tanning of shark skin, shark cartilage<br />

Shrimp waste, crab shell and squilla utilisation: Resources and composition, conventional<br />

uses, feeds and manure, conversion to useful materials like chitin, chitosan, glucosamine<br />

hydrochloride, commercial production, production and use of protein isolates from squilla<br />

and shrimp waste.<br />

<strong>Fish</strong> protein concentrate: Different methods of production, functional properties, different<br />

types of FPC, texturised products.<br />

<strong>Fish</strong> silage: Acid silage and fermented silage, advantages over fish meal, nutritional value of<br />

silage.<br />

<strong>Fish</strong> hydrolysates: Production and utilisation, biochemical composition and importance in<br />

food and nutrition<br />

Miscellaneous by-products: <strong>Fish</strong> maws and isinglass, pearl essence, fertiliser, beche-demer,<br />

processing of snail meat and jelly fish.<br />

Practical:<br />

Preparation of fish meal, FPC, fish oils, chitin, chitosan, glucosamine hydrochloride, fish<br />

maws, isinglass, agar, alginic acid, , glue, pearl essence, fish sauce.<br />

Reference:<br />

• Advances in <strong>Fish</strong> processing <strong>Technology</strong>, Sen D. P., Pub. Allied Publishers Pvt. Ltd.<br />

New Delhi (2005).<br />

• Postharvest <strong>Technology</strong> of fish and fish products. (2001). By Balachandran, K. K.,<br />

Pub. Daya Publishing House, Delhi,<br />

18/4/08 16


• <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong> (1992) G. M. Hull. (Ed), Blackie Academic and<br />

Professional, London<br />

• Govindan, T. K., <strong>Fish</strong> processing <strong>Technology</strong>, Pub. Oxford & IBH Publishing Co.,<br />

New Delhi (1985).<br />

• Principle of Food Science. Fennema, O. R. (editor), Publ. Marcel Dekker Inc.<br />

NewYark<br />

• Text Book of <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong>; Gopakumar K. (editor), Pub. ICAR, New<br />

Delhi (2002)<br />

• Introduction to <strong>Fish</strong>ery Byproducts (1981) By Barlaw<br />

• <strong>Processing</strong> Aquatic Food Products, By Wheaton and Lawson (1985) John Wiley and<br />

Sons New York<br />

Design, maintenance of fish processing plants and instrumentation 1 + 1<br />

Objective:<br />

• To teach plant design, function and maintenance of fish processing plants and<br />

various measurement techniques<br />

• To teach scientific techniques of effluent treatment<br />

Theory:<br />

a) Design and maintenance of fish processing plants<br />

Plant design: Fundamentals of processing plant design: Site selection, design and<br />

preparation of layout of processing plants - freezing plant, cold storage, canning plant,<br />

dryers etc.<br />

Functions and construction of refrigeration system: Tests and inspection, Operation and<br />

handling, P-H diagram and basic calculation - Application of P-H diagram, size and required<br />

power of compressor, maintenance of refrigerating machine, troubles and causes.<br />

18/4/08 17


Preventive maintenance of machinery and equipment of fish processing plants, IQF,<br />

Canning plant, sausage plant, artificial dryers, smoking chambers etc., safety controls for<br />

freezing and canning plant<br />

Effluent treatment: Legislation and standards of effluent discharge, water pollution control<br />

measures in the food industry, waste water treatment process; dissolved air floatation,<br />

sedimentation, chemical treatment, biological treatment, aeration, carbon adsorption,<br />

granular media filtration and sludge handling. Boilers - Classification and selection of<br />

boilers, Boiler mounting and accessories.<br />

b) Instrumentation<br />

Measurement techniques<br />

Sensors, active and passive sensors, characteristic of sensors for the measurement of<br />

temperature, relative humidity, a w value, gel strength, moisture, freshness, pH, conductivity,<br />

DO, redox potential, salinity, air velocity, solar energy and brine concentration.<br />

Thermometers: Different types of thermometers, characteristics and application.<br />

Instrumentation techniques: General configuration of instrumentation system.<br />

Instrumentation for measurement of a w value, temperature, pH, freshness, gel strength,<br />

salinity, brine concentration<br />

Thermal properties of foods: Calorie, heat loss, heat gain, specific heat, Newton's laws of<br />

cooling, heat transfer, latent heat, laws of fusion, thermal conductors, thermal diffusivity<br />

Practical:<br />

a) Design and Maintenance of <strong>Fish</strong> <strong>Processing</strong> Plants<br />

Operation and maintenance of machinery and equipment for cold storage plant, freezing<br />

plant, canning plant, sausage making, dryers, boilers etc. Assembly of a refrigeration unit<br />

and charging refrigerant.<br />

b) Instrumentation<br />

Measurement of temperature inside cold storage / freezer, fish during freezing and thawing.<br />

18/4/08 18


Estimation of Gel strength.<br />

Measurement of solar radiation, air velocity, air temperature. Measurement of salinity,<br />

conductivity, pH.<br />

Estimation of water activity.<br />

Reference:<br />

• Advances in <strong>Fish</strong> processing <strong>Technology</strong>, Sen D. P., Pub. Allied Publishers Pvt. Ltd.<br />

New Delhi (2005).<br />

• <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong> (1992) G. M. Hull. (Ed), Blackie Academic and<br />

Professional, London<br />

• Principle of Food Science. Fennema, O. R. (editor), Publ. Marcel Dekker Inc.<br />

NewYark<br />

Microorganisms of public health significance 1+1<br />

Objective:<br />

• To teach detail about microorganisms of public health significance<br />

• To teach regarding food-born diseases and their prevention<br />

• To teach food causing bacteria, virus and parasites.<br />

Theory:<br />

Infection and immunity, microbial food poisoning, bacteria of public health significance in<br />

fish I fishery products I environments - Salmonella, Clostridia, Staphylococcus, E. coli,<br />

Streptococcus, Vibrio, Aeromonas, Listeria, Yersinia, Bacillus. Laboratory techniques for<br />

detection and identification of food poisoning bacteria. Mycotoxins in cured fish.<br />

Food-borne bacterial infections.. Food infections by Salmonella, Clostridium perfringens,<br />

Vibrio parahaemoliticus, Enteropathogenic E. coli, Aeromonas hydrophila etc., the nature of<br />

causative agent, its source, incidence, foods involved, the diseases, conditions for outbreak<br />

and prevention. The etiology of diseases: Conditions for outbreak & prevention<br />

18/4/08 19


Botulism and staphylococcal food poisoning, organism responsible and their origin, growth<br />

and toxin production, nature of toxins, incidence of poisoning, foods involved.<br />

Food borne non-bacterial infections and intoxications: Aflatoxins, patulin, ochratoxin and<br />

other fungal toxins found in food, toxin producer, source, nature of toxin, toxicity and<br />

significance in foods.<br />

Virus and some parasites found in foods.<br />

Practical:<br />

Laboratory techniques to detect and identify pathogens in fish - E.coli, Staphylococcus<br />

aureus, Streptococus faecalis, Clostridium perfrigens, Clostridium botulinum, Salmonella,<br />

Listeria, Vibrio cholera, Vibrio parabaemolyticus, V. vulnificus, Animal bio-assay of bacterial<br />

toxins.<br />

Reference:<br />

• Food Borne Pathogens Varnam & Evans<br />

• Food Borne Disease Handbook. IInd Edn. (2001) Vol.4:Seafood and Environmental<br />

Toxins, Marcel Dekker Inc New York<br />

• Food Borne Infections & Intoxications Reimann & Bryan<br />

• Food Microbiology Fundamentals & Frontiers M.P. Doyle<br />

• General Microbiology Hans H Schelgel<br />

• Handbook of Natural toxins - Vol 3. Marine Toxins and Venom. 1988. Marcel Dekker<br />

Inc. New York<br />

• Handbook of Natural toxins - Vol 4. Bacterial Toxins. 1988. Marcel Dekker Inc. New<br />

York.<br />

• Microbiology Perscott Harvey & Cleim<br />

• Seafood toxins 1984. American Chemical Society, Washington, D.C.<br />

18/4/08 20


<strong>Fish</strong>ing <strong>Technology</strong> 2+1<br />

Objective:<br />

• To impart training in design, fabrication and operation of fishing gears.<br />

• To teach the importance and guidelines regarding code of conduct for responsible<br />

fishing operations and safety at sea.<br />

Theory:<br />

Design, fabrication and operation of various fishing gears: trawls (pelagic and bottom),<br />

purse seine, gillnets, trammelnets, dolnets, FADs (Floating and bottom – artificial reefs),<br />

traps and lines.<br />

Harvesting methods in inland water bodies and their improvisation: Gillnets, castnets, lines,<br />

dragnets, bagnets etc.<br />

Effects of fishing method and on-board handling on fish quality<br />

Destructive and prohibited fishing practices.<br />

By-catch reduction devices: Definition of by-catch, types of by-catch reduction devices and<br />

the principles of operation.<br />

Turtle Excluder Devices: Definition, types of TEDs – soft and hard types, materials used for<br />

construction, maintenance.<br />

Acoustics: Acoustic surveys for fishing, acoustic aids in fishing and acoustic measurements.<br />

Safety at sea: Safety devices – Accidents at sea, mitigation measures. Safety devices -<br />

SART, EPIRB, GMDSS.<br />

Advanced communication Systems – VHF, SSB, Inmarsat System.<br />

Vessel Monitoring Systems (VMS): Importance, uses, role in fisheries management.<br />

Satellite navigation system: GPS – Components of GPS, working, functions, , important<br />

applications of GPS in fisheries and aquaculture.<br />

18/4/08 21


<strong>Fish</strong>ing harbours: Classification, facilities, layout of a typical fishing harbour, stages in the<br />

planning of fishing harbours.<br />

Code of Conduct for Responsible <strong>Fish</strong>eries (CCRF): Articles of CCRF, Elaboration of Article<br />

8: <strong>Fish</strong>ing Operations.<br />

Practical:<br />

Drawing and reading gear designs.<br />

Field visits to fishing harbour and preparation of drawing of its lay out.<br />

Training onboard fishing vessels in fishing techniques, familiarization with navigation and<br />

communication equipments,<br />

Handling and storage of fish.<br />

Study of structure and operation of a fish landing centre.<br />

Study of destructive fishing techniques<br />

<strong>Fish</strong> aggregating devices.<br />

Familiarization with safety devices like SART, EPIRB and GMDSS.<br />

Reference:<br />

• An account of the inland fishing gear and methods of India. (1971). George VC.,<br />

Indian Council of Agricultural Research. Special Bulletion of <strong>Central</strong> <strong>Institute</strong> of<br />

<strong>Fish</strong>eries <strong>Technology</strong>, Ernakulam (9), pp. 68.<br />

• Calculations for fishing gear designs, (1986) Fridman, A.L., FAO <strong>Fish</strong>ing manual,<br />

<strong>Fish</strong>ing News Books, Ltd., Farnham, 264 p.<br />

• Commercial fishing methods-an introduction to vessels and gear, (1996).<br />

Sanisbury, J.C., <strong>Fish</strong>ing News Books Ltd., Farnham, 352 p.<br />

• Course manual in <strong>Fish</strong>ing <strong>Technology</strong>, (1988). Shenoy, Latha., CIFE, Mumbai, 95 p.<br />

18/4/08 22


• <strong>Fish</strong> catching methods of the world. (1984). Brandt. A.V., <strong>Fish</strong>ing news books Ltd.,<br />

London: 432 p.<br />

• <strong>Fish</strong>eries acoustics, <strong>Fish</strong> and <strong>Fish</strong>eries Series 5, (1992) MacLennan, D.N. and<br />

Simmonds, E.j., Chapman and Hall, London, 323 p.<br />

• <strong>Fish</strong>erman’s workbook, (1990), Prado, J., <strong>Fish</strong>ing News Books, Blackwell Scientific<br />

publications Ltd., Osney Mead, Oxford, 192 p<br />

• <strong>Fish</strong>ing gear and craft technology. (2001). Sreekrishna, Y. and Shenoy Latha.,<br />

Indian Council of Agricultural Research, New Delhi, 342 p.<br />

• <strong>Fish</strong>ing operations, (1996). FAO Training guidelines for responsible fisheries No. 1,<br />

FAO, Rome, 26 p.<br />

• Modern <strong>Fish</strong>ing Gear <strong>Technology</strong>, (2000) Hameed, M.S. and Boopendranath, M.R.,<br />

Daya Publishing House, Delhi:186 p.<br />

Handling, storage and transport of fresh fish 1 +1<br />

Objective:<br />

• To teach scientific techniques handling, storage and transport of fresh fish<br />

• To teach various post harvest changes during chill storage of fish.<br />

Theory:<br />

Structure of fish myosystems, Postmortem changes - Structural and chemical<br />

<strong>Fish</strong> as raw material for processing: Body structure, physical properties, shape, specific<br />

weight, bulk weight, angle of slip, weight composition.<br />

Factors affecting quality of fresh fish: intrinsic and extrinsic factors.<br />

Handling of fish onboard fishing vessels, Unit operations<br />

Unloading fish, <strong>Fish</strong> pumps<br />

18/4/08 23


Post-harvest <strong>Fish</strong>ery losses, Methods to reduce losses<br />

Handling of fish in landing centres, defects and modifications needed<br />

Chill storage of fish: Heat load calculation, storage methods. insulated boxes and insulation<br />

thickness, different types of ice, physical, chemical, microbiological and sensory changes<br />

during chill storage, iced storage shelf life, cold shock, physical, chemical and sensory<br />

methods of analysis.<br />

Different types of ice and their manufacture, Flow ice<br />

Sous-vide technology<br />

Melanosis and its prevention, discolouration in aquatic products, non- enzymatic browning<br />

Depuration of bivalves<br />

Modified Atmosphere Packaging<br />

Transportation: Live fish/shell fish, Transportation of raw fish to local markets and<br />

processing centres, Improvements needed in transportation, Refrigerated transport<br />

systems, Classification of transport vehicles, Cold chain.<br />

Practical:<br />

Chill storage studies: Chemical, physical and sensory analysis, determination of shelf life<br />

Handling of fish, bivalves, prawns, mollusks<br />

Depuration, treatment with chemicals, evaluation of freshness of fish.<br />

Reference:<br />

• Advances in <strong>Fish</strong> processing <strong>Technology</strong>, Sen D. P., Pub. Allied Publishers Pvt. Ltd.<br />

New Delhi (2005).<br />

• Assessment and Management of Seafood Safety and Quality. (2003) Free amino<br />

acids. FAO Technical Paper No. 444<br />

18/4/08 24


• <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong> (1992) G. M. Hull. (Ed), Blackie Academic and<br />

Professional, London<br />

• Food Storage Stability, CRC Press, New York<br />

• Food Refrigeration Processes (1990) By Cleland C Andrew, Elsevier Applied<br />

Sciences, London<br />

• Freezing Effects on Food Quality, Marcel Dekker, Inc. New York<br />

• Govindan, T. K., <strong>Fish</strong> processing <strong>Technology</strong>, Pub. Oxford & IBH Publishing Co.,<br />

New Delhi (1985).<br />

• Low Temperature Preservation of Foods and Living Matter Marcel Dekker, Inc. New<br />

York<br />

• Post-harvest <strong>Technology</strong> of fish and fish products. (2001). Balachandran, K. K., Pub.<br />

Daya Publishing House, Delhi,<br />

• Principle of Food Science. Fennema, O. R. (editor), Publ. Marcel Dekker Inc.<br />

NewYark<br />

• Principles of Total Quality. (1994) St. Leuie Press, USA.<br />

• Text Book of <strong>Fish</strong> <strong>Processing</strong> <strong>Technology</strong>; Gopakumar K. (editor), Pub. ICAR, New<br />

Delhi (2002)<br />

<strong>Technology</strong> of mince based fish products 1+1<br />

Composition of muscle proteins in fish and their role in emulsification and elasticity<br />

formation<br />

Factors influencing denaturation of muscle proteins and their theories.<br />

testing the protein denaturation<br />

Methods of<br />

Factors influencing elasticity formation and theories of gel formation. Minced meat from<br />

different varieties of fresh and marine water fishes.<br />

18/4/08 25


Improvement of colour of meat by bleaching and certain additives. Use of antidenaturants<br />

to prevent and denaturation of proteins of fish mince during storage.<br />

Changes in meat during mincing and mixing operations and cooking and setting<br />

phenomena.<br />

<strong>Technology</strong> of processing and preservation of gel forming fish flour (AFPP), its property<br />

and utilisation. Unit operations in analog product preparation- Crab sticks analogs,<br />

moulded lobsters and crabs<br />

Battered and breaded products<br />

Use of emulsifiers, binders, seasonings, spices, antioxidants, smoke extract,<br />

Preservatives, natural and artificial casings, nitrites and nitrates. Fortification of fish<br />

products with vitamins and minerals. Quality standards and recent developments.<br />

Practicals: Measurement of viscosity of fish proteins by Ostwald viscometer, effect<br />

of water washing on the quality of meat, colour fixation of red colour meat and estimation<br />

of nitrite. Studies on setting of fish meat. Estimation of starch in the final paste product.<br />

Fundamentals of controlled stress Rheometer. Effect of two stage heating of fish sol on<br />

gel strength.<br />

ADDITIVES IN FISH PROCESSING 1+1<br />

Objectives :<br />

• To familiarize use of different additives, their effects, levels and detection.<br />

Theory<br />

Introduction to food additives-definition-technical benefits of food additives-intentional<br />

and incidental additives.<br />

Relationship of great revolutions in history to the development of food additives –<br />

Agricultural Revolution-Industrial revolutions – urbanization. Basic uses of food<br />

additives.<br />

18/4/08 26


Intentional additives – use of specific nutrients as food additives – Requirements and<br />

considerations – minerals. Vitamins, amino acids and nutrient concentrates as<br />

additives.<br />

Policy considerations in the use of food additives. Flavours and colour as additives.<br />

Antioxidants – Mechanism of antioxidants; commercial antioxidants and selections.<br />

Analytical methods for antioxidants.<br />

Acidulants in food processing; Sequestrants in food processing; Polyphosphates in<br />

food processing; Starch as an additive; Incidental additives.<br />

Practicals<br />

Determination of food additives such as preservatives, antioxidants, curing agents,<br />

chelating agents, acidulants and phosphates in various food products. Detection of<br />

certain intentional and unintentional food additives in foods.<br />

PACKAGING TECHNOLOGY FOR FISH AND FISHERY PRODUCTS 1+1<br />

Objectives :<br />

• To acquaint with different packaging materials, their appropriate use and benefits<br />

Theory:<br />

Food packaging, its purposes and procedures; technological aspects of<br />

packaging fishery products; packing of fresh and frozen fish for consumers; packaging<br />

for transport, shipping and institutional supplies; packaging standards for domestic and<br />

international trade.<br />

Packaging materials; basic films and laminates, their manufacture and<br />

identification; resistance of packaging materials, causes and remedial measures;<br />

development of protective packaging for fishery products; methods of testing for<br />

packaging materials for their physical properties; containers and their testing and<br />

18/4/08 27


evaluation; package designs; resistance of packages to hazards in handling; transport<br />

and storage.<br />

Labelling and printing of packaging materials.<br />

Practicals: Assessment of quality parameters such as moisture permeability, grease<br />

resistance, thickness/guage of basic plastic films and laminates.<br />

Quality assessment of paper and board and the products prepared from them.<br />

Evaluation of packages with regard to the resistance to handling, transportation and<br />

storage.<br />

<strong>PhD</strong> <strong>Syllabus</strong><br />

CORE COURSES<br />

Biochemical Techniques in <strong>Fish</strong> Analysis 2 + 1<br />

Objective:<br />

• To teach detail about various biochemical techniques in fish analysis<br />

Theory:<br />

General principles of separation of micro and macro molecules, selection of appropriate<br />

tools for analysis of fish samples. Outlines of common techniques involved in biochemical<br />

analysis.<br />

Centrifugation techniques: types of centrifugation, concept of Svedberg unit, analytical<br />

ultracentrifuge.<br />

Filtration technique: different types of filtration, types of filters and means of using them.<br />

Spectroscopic techniques: Principles, UV-Visible and IR spectroscopy, spectro-fluorimetry,<br />

18/4/08 28


flame photometry, atomic absorption spectrophotometry, ICP- AES, mass spectrometer<br />

Electrophoretic techniques :<br />

General principles. Classification, Paper electrophoresis,<br />

Native and reduced PAGE, IEF, capillary electrophoresis, 2D Gel electrophoresis.<br />

Chromatographic Techniques : General principle, types of chromatography - adsorption,<br />

partition, ion-exchange, molecular sieve, affinity, Gas chromatography, thin layer<br />

chromatography.<br />

High performance Liquid chromatography, LC MS-MS: Theory and instrumentation.<br />

Practical<br />

Isolation of proteins: sarcoplasmic, myofibrillar, and stromal.<br />

Estimation of proteins : Biuret, Lowry and Dye binding technique.<br />

Amino acid analysis, non-protein nitrogen.<br />

Extraction and estimation of lipids: Measurement of oxidation and hydrolysis of lipids, Fatty<br />

acid profile.<br />

Minerals and heavy metals: Estimation by Atomic Absorption Spectroscopy and flame<br />

photometer.<br />

HPLC- determination of histamine<br />

Demonstration of GC-MS-MS,<br />

Separation of protein by electrophoresis<br />

Reference<br />

1. Introductive Spectroscopy, (1979) Pavia Lampman, Kriz Saunder College Publishers,<br />

2. Mass Spectrometry in Biological Sciences (1996) A.C. Burlingame & S.P. Carr<br />

3. Introduction to Analytical Gas Chromatography (1981), J.A, Peary, Marcel Dekker Inc. 4,<br />

Analytical Instrumentation Handbook (1997), G.W.Ewing, Marcel Dekker Inc.<br />

5. Analysis of Food for nutrition labeling & Hazard contaminants (1995), I.J. Jean & W.J.<br />

Ikim, Marcel Dekker Inc -<br />

6. Practical Biochemistry - Principles & Techniques (2000) Keith Wilson &John Walker<br />

Cambridge University Press<br />

18/4/08 29


7. Mass Spectrometry of Biological materials (1988) B.S.Larsen & C.N. McEwen, Marcel<br />

Dekker Inc<br />

Functional properties of proteins from fish and shellfish 2+1<br />

Objective:<br />

To teach students on biochemical properties that are known to affect product properties.<br />

Theory:<br />

Definition of functional properties and their importance in proteins from fish.<br />

Typical<br />

functional<br />

properties of proteins in food system.<br />

Protein structure and function:<br />

Protein folding and non-covalent forces stabilizing protein<br />

structure with special reference to hydrophobic interactions. Free energy and entropy<br />

concept in relation to hydrophobic interaction. Surface hydrophobicity and its relation to<br />

functional properties. Estimation of surface hydrophobicity and total hydrophobicity.<br />

Solubulity and water sorption of proteins: Factors affecting protein hydration. Viscosity in<br />

relation to protein hydration: Methods of estimating viscosity.<br />

Gelation : Definition of gel, mechanism of formation of gel, factors affecting the gel<br />

formation. Evaluation of gelling capacity- thermal, rheological and microscopy.<br />

Surfactant properties: emulsifying and foaming, Importance of emulsifying properties of<br />

proteins. Theoretical concept of emulsion capacity and stability. Interfacial properties,<br />

adsorption from solution. Methods of estimating surface tension.<br />

18/4/08 30


Emulsion instability: Creaming, sedimentation, aggregation vs Brownian aggregation.<br />

DLVO theory, microemulsions. Methods for estimation of emulsion capacity and stability.<br />

Macromoleculear absorption and different stages of foaming. Foam stability in relation to<br />

proteins structure. Foaming ability of different protein systems with case studies.<br />

Denaturation and functionality: Changes in functional properties of proteins as affected by<br />

icing, freezing, drying, salting and heating.<br />

Modification of proteins for improving<br />

functionality- Succinylation and acetylation procedures.<br />

Practicals<br />

Evaluation of different functional properties like water absorption, fat absorption,, gelling,<br />

emulsification capacity and stability of fish/shell fish proteins. Effect of pH, temperature and<br />

ionic strength on various functional properties. Prediction of functional properties using<br />

model compounds.<br />

Quality Management System (2+1)<br />

Objective:<br />

• To teach different aspects of quality management system and evaluation techniques<br />

for seafood<br />

• To teach Seafood Quality Assurance and Quality Assurance Systems<br />

Theory<br />

Quality Management Systems: The concept of total quality management. The principles of<br />

TQM. Zero defect planning, Quality circle, Quality link, Quality culture. Statistical Quality<br />

Control. Quality as related to preprocess handling, transportation, processing and storage.<br />

Quality evaluation techniques for seafood: Physical, chemical. bacteriological and<br />

Instrumental methods of quality evaluation. Sensory evaluation.<br />

18/4/08 31


Quality standards: National and International – Codex, USFDA, EU norms, ISO, BIS etc.<br />

standards for fish and fishery products.<br />

Seafood Quality Assurance and Quality Assurance Systems: Good Manufacturing (GMP)<br />

and Good Hygiene Practices (GHP) -Codex guidelines. The concept of HACCP in seafood<br />

safety. HACCP team Management role and CCPs and implementation procedure for<br />

HACCP- ISO 22000 FSMS. ISO 9000 series of standards. Cold schedule and hotschedule<br />

for handling perishable commodities.<br />

Validation of methods for quality assurance- Method selection, Quality check, inter-lab<br />

comparision, proficiency testing. Primary standards. Reference standards. Reference<br />

material (RM), Certified Reference Material (CRM) and Standard Reference Material (SRM),<br />

Uncertainty and Calculation of Uncertainty of Measurements<br />

Sample Accountability: Sampling plan -probability sampling and non- probability sampling..<br />

Practical:<br />

Developing flow charts and exercises in identification of hazards- preparation of hazard<br />

analysis worksheet, plan form and corrective action procedures in processing of fish.<br />

Analysis of typical hazards, study of correction and corrective action. Detection and<br />

estimation of important toxic chemicals in food, quality defects.<br />

Reference<br />

1. Assessment and Management of Seafood Safety and Quality. (2003) FAO<br />

Technical Paper No. 444<br />

2. Principles of Total Quality. (1994) St. Leuie Press, USA.<br />

3. TQM - in New Product Manufacturing. (1992), McGraw Hill Inc. New York 4. Introduction<br />

of Total Quality. (1994) Prentice Hall Inc., USA<br />

5. ISO- 9000-3 1995 Springer<br />

6. Food Borne Disease Handbook. (2001) Marcel Dekker.<br />

OPTIONAL COURSES<br />

18/4/08 32


Lipids of Aquatic Origin 2+1<br />

Objective:<br />

• To teach aquatic originated lipids, their metabolic activities and biological significance<br />

Theory:<br />

Lipid classification: Triglycerides, Phospholipids, steroids and other lipids. Lipid micelles and<br />

bilayer.<br />

Fatty acids : Classification, stereochemistry, nutritional significance of fatty acids<br />

Source of lipids : Biosynthesis of lipids, lipid metabolism including that of phospholipids,<br />

typical properties of marine lipids.<br />

Lipids in Biological membranes : Membrane proteins, lipoproteins, transport across<br />

membranes.<br />

Lipid metabolism: fatty acid oxidation, ketone bodies, lipid biosynthesis, regulation of<br />

cholesterol metabolism. Biological significance of marine lipids. Ether lipids and<br />

Eicosanoids- their significance.<br />

Modern analytical techniques employed in lipid chemistry. Methods of extracting polyunsaturated<br />

fatty acids<br />

Practical<br />

Extraction and fractionation of lipids. Fatty acid composition of different lipid fractions.<br />

Evaluation of oxidation product of fish lipid during processing and storage.<br />

Reference<br />

1. Biochemistry, Zybey<br />

2. Fundamentals of Biochemistry (1998) : DVoet, J.G. Voet & C.D. Praff, John Weily<br />

Intersicence,<br />

3. Food Lipids, CC. Akoh & D.B. Min, 1998, Marcel Dekker Inc<br />

4. Nutrition (2001), Paul Jnsel, R.E. Turna & D.Ross, Jones & Bartlet Publishers<br />

18/4/08 33


5. Food Biochemistry & Nutritional Value (1987), David S. Simpson, Longman Scientific &<br />

Technical<br />

Microbial Hazards in <strong>Fish</strong> <strong>Processing</strong> (2+1)<br />

Objective:<br />

• To teach various microbiological related hazards in fish processing.<br />

Theory:<br />

Public health microbiology- Food borne pathogens : Salmonella, Shigella, Enteropathogenic<br />

E. coli, Clostridium botulinum, Listeria monocytogenes, Staph aureus and Vibrio<br />

cholerae, V. parahemolyticus. Emerging food-borne pathogens. Water- borne, Air-borne<br />

and food-borne diseases.<br />

Microbial virulence- infectious diseases. Virulence.<br />

Microbial toxin production-opportunists and true pathogens<br />

Methods for Detection : Rapid detection and indirect detection methods of pathogens and<br />

parasites. Method validation.<br />

Antimicrobial systems and food preservation: ecological concepts: Lactoperoxidase. Nisin,<br />

Lysozyme, Bacteriocins.<br />

Norms for using antimicrobial systems in food processing and preservation. Food Safety,<br />

Risk analysis. Potential health hazards and risks associated with fish products.<br />

Packaging and modified atmosphere on the microbiology and shelf life of fishery products.<br />

Predictive modeling in quality and safety assurance of fishery products.<br />

Practical<br />

Antibiotic assay, sensitivity tests, evaluation of antibacterial properties. Analysis of fish<br />

product constituents. MIC, MCC, Methods for Risk analysis of seafood.<br />

18/4/08 34


Reference<br />

1. Food Borne Bacterial Pathogens<br />

2. Food Borne Infections & Intoxications Reimann & Bryan<br />

3. Food Microbiology Fundamentals & Frontiers M.P. Doyle<br />

Colour and flavour bearing constituents of aquatic organisms (2+1)<br />

Objective<br />

• Compounds responsible for flavor and colour of fish and shellfish.<br />

Theory<br />

Vitamins, Minerals, Pigments, Flavour bearing constituents and other Components in<br />

Aquatic Organisms<br />

Vitamins: Metabolic functions of vitamins, water soluble and fat soluble vitamins<br />

Vitamins from sea food<br />

Minerals: Role of trace elements in metabolism, trace elements of sea food, toxic heavy<br />

metals in sea food.<br />

Pigments and flavour bearing compounds of aquatic origin, chemistry, biochemical role,<br />

changes during processing of seafood.<br />

Metabolic functions of hormones<br />

Nucleoprotein, nucleic acids, nucleotides, nucleosides.<br />

Practicals<br />

Modern methods for analysis of vitamins, minerals and nucleic acids<br />

Organoleptic evaluation of flavours and pigments<br />

Extraction of flavours and pigments and evaluation<br />

18/4/08 35


Toxins and contaminants (2+1)<br />

Objective:<br />

• To teach about various types of toxins and contaminants and their tolerance limit<br />

• To teach various analytical methods to estimate toxins and contaminants<br />

Theory:<br />

Public health problems due to food borne contaminants<br />

Factors contributing to outbreaks of food poisoning<br />

Aflatoxins in fishery products. PAH in smoked fish. Biogenic amines and its significance to<br />

human health, Different types of marine bio-toxins such as Ciguatoxin, Paralytic shellfish<br />

toxins Diarrhetic shell fish toxins, DSP toxins, Scomberotoxins, Brevitoxins, etc. Symptoms,<br />

treatment, pharmacology, detection,<br />

Overview of toxicity of marine animals.<br />

Analytical methods for different types of marine toxins and its tolerance limits : Stability,<br />

Bioassays, Pharmacology assays, Immunoassays, Instrumental methods.<br />

Contaminants of the Aquatic Environment - Heavy metals (Hg, Cd, Pb, Cr, Ni, As etc.).<br />

Pesticide contaminants : PCB, organochlorine etc., their source, bioaccumulation,<br />

magnification and toxicity. Persistent pollutants. Toxicity Evaluation. Measurement of LC 50<br />

and factors affecting LC 50 , Animal tissue Analysis.<br />

Practical<br />

Analysis of bacterial and fungal toxins<br />

Analysis of heavy metals and common pesticides<br />

Biogenic amine estimation<br />

Estimation of LC 50<br />

Reference<br />

1. Food borne Disease Handbook. IInd Edn. (2001) Vol.4:Seafood and Environmental<br />

Toxins, Marcel Dekker Inc New York<br />

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2. Seafood toxins 1984. American Chemical Society, Washington, D.C.<br />

3. Handbook of Natural toxins - Vol 3. Marine Toxins and Venom. 1988. Marcel Dekker Inc.<br />

New York<br />

4. Handbook of Natural toxins - Vol 4. Bacterial Toxins. 1988. Marcel Dekker Inc. New York.<br />

Labeling of fish and fishery products (2 +1)<br />

Objective<br />

• Labeling of different products, guidelines and enforcement.<br />

Theory<br />

Labeling requirements - national and international, legislation on labeling.<br />

Labeling for product traceability,<br />

Components of traceability code – nutrition facts and nutrition labeling, specific<br />

requirements of nutrition labeling, food meant for specific age groups and convalescing<br />

people.<br />

Serving size, calculation of nutrition facts based on nutrient composition and serving size.<br />

Type of labeling for organic foods specific foods like organic foods, GM foods, irradiated<br />

foods, vegetarian and non vegetarian foods.<br />

Label design specification – size, colour,<br />

Major nutrients Minor nutrients, Essential nutrients, Function (or note) of nutrients -<br />

(providing energy, tissue building) Nutritional research - Nutritional aspects of fish proteins,<br />

lipids, vitamins and free minerals Functional foods/ Neutraceuticals for health, Effect of food<br />

processing on nutritive values of foods. Antinutritional factors, Nutrition labeling, (Energy<br />

value of foods)<br />

Practical<br />

Analysis of major and minor nutrients, calculation of nutrition facts, preparation of labels for<br />

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typical food items.<br />

Analysis for total calorie, calorific value of fats, protein and carbohydrates.<br />

PER, BV, NPU analysis of different products.<br />

Reference<br />

1. Introduction to Clinical Nutrition & Nutritional Labelling (1998), Viswanathan Seshadri<br />

Marcel Dekker Inc.<br />

2. Nutrition (2001), Paul Jnsel, R.E. Turna & D.Ross, Jones & Bartlet Publishers<br />

3. Food Biochemistry & Nutritional Value (1987), David S. Simpson, Longman Scientific &<br />

Technical<br />

Environmental Impacts of <strong>Fish</strong>eries Industries 2+1<br />

Objective:<br />

• To teach different aspects of Environmental Management Systems in fisheries<br />

industry<br />

Theory<br />

Environmental Management Systems: Environmental issues, (Ozone depletion, global<br />

warming etc.) pollution, long term ecosystem degradation etc in aquaculture and<br />

processing industries.<br />

Environmental impact assessment studies of fisheries industry and control measures,<br />

Sources of environmental concerns (Physical, Chemical and Microbiological).<br />

Techniques for the identification of environmental aspects. IS/ISO 14000 and its relevance<br />

to Environmental Management System in fisheries industry: Background, Policy and<br />

Planning, Implementation, Checking and Review, International and European Laws for<br />

Environmental Protection, National Environmental Laws.<br />

Practical<br />

Composition analysis of fish processing waste, analysis of pollution aspects of solid and<br />

liquid wastes – bacterial load, TDS, BOD, COD, pH, temperature, oil and grease . Resident<br />

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time analysis for processing waste at the site of disposal.<br />

Reference<br />

1. Bureau of Indian Standards: IS/ISO 14000:1996 on Environmental Management System<br />

2. US-EPA 2000, Manual of Chemical Methods - Second Edition<br />

3. Process Engineering for Pollution Control and Waste Minimization 1994. Ed.<br />

Donald L. Wise Pub!. By Marcel Dekker.<br />

4. APHA 1998 Standard methods for Examination of water and waste water Ed.<br />

Cesceri, L.S.<br />

5. Manual of Environmental Microbiology, Second Edition 2002, Ed. Hurst C., J.<br />

ASM Press<br />

<strong>Fish</strong>ery by-products, specialty products and value added products (2+1)<br />

Objectives<br />

• To teach students the preparation of products from low cost fish.<br />

Theory<br />

Nutritional importance of fish meal and quality requirements -Raw material quality<br />

Nutritional importance of fish oil and methods to impart stability to fish oils on storage,<br />

Unsaponifiables in fish liver oils<br />

Production of fish flour, quality standards and applications.<br />

Different methods of production of FPC, Different types of FPC, and their specifications.<br />

Enzyme hydrolysis of fish, fish hydrolysates, fish peptones, hydrolysates enriched food<br />

beverages<br />

Food flavour from tiny prawns and non-penaeid prawns<br />

Formulation of pet food<br />

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Chitin, Chitosan and protein extract from shrimp and crab shell and squilla, Quality<br />

requirements and assessment of chitin and chitosan, Application of chitin and chitosan.<br />

Conversion of chitin and chitosan to high value products – glucosamone hydrochloride,<br />

glucosamine sulphate and their use.<br />

Extraction of collagen from fish processing wastes, properties and application. Preparation<br />

of biological membranes using collagen and chitosan for biomedical applications.<br />

Value Added Products: Present market trends, scope of value addition, Types of value<br />

addition, Important value added products.<br />

Coated products – Principles and type of coating, Coating functions, in gradients, Batter<br />

classification, Mechanical properties of batter, Bread crumbs, Flavorings, Seasonings and<br />

Hydrocolloids in coatings, Fat and oils in Coated food and their chemistry,Trouble shooting<br />

techniques for batter and breading systems, Application of batters and breading to seafood.<br />

Practical<br />

Preparation of glucosamine hydrochloride and glucosamine sulphate.<br />

Preparation of isinglass, collagen powder and collagen and chitosan. Preparation of fish<br />

wafers, fish fingers, cutlets etc.<br />

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