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PPl THE PHYSIOLOGICAL ROLES OF THE BOAR EJACULATE H. Rodríguez-Martíne1, u. Kuist', F. saravial, M. wallgrent'', A. Johannissono, L. sanrs, F.J. Peña6, E.A. Martín ez' , J. Roca7, J.M. Vázqu ez7 , andJ.J.-Calvetes lDiuision of Reproduction, Faculty of Veterinary Medicine & Animal Science (FVMAS), Swedish University of Agricultural Sciences (SLU), POB 7054, SE-75007 Uppsala, Sweden. 'Center for Andrology & Sexual Medicine, Departm-ent of Medicine, Karolinska University Hospital/Huddinge, SE-14186 Stockholm, Sweden; 3Quality Genetics, Råby 2004, SE-24292 Hörby, sweden; aDepartment of Anatomy, Physiology & Biochemistry, FVMAS, sLU, poB 7011, SE-75007 Uppsala, Sweden; slaboratory of Structural Proteomics, Institute of Biomedicine of valencia, csIC, Jaime Roig 1 l, 46010 valencia, spain; 6section of Reproduction & Obstetrics, Department of Medicine, Faculty of Veterinary Medicine, Avd de la Universidad s/n, 10071 Cáceres, Spain;TDepartment of Medicine & Animal Surgery, Faculty of Veterinary science, university of Murcia, campus de Espinardo, 30100 Murcia, Spain. E-mail: heriberto.rodriguez@kv.slu.se During ejaculation in the boar, sperm cohorts emitted in epididymal cauda fluid are sequentially exposed and resuspended in different mixtures of accessory sex gland secretion. This paper reviews the relevance of such unevenly composed fractions of seminal plasma (SP) in vivo on sperm transport and sperm function and how this knowledge could benefit boar semen processing for arlificial insemination (AI). The firstly ejaculated spermatozoa (first 10 mL of the sperm-rich fraction, SRF tPll) remain mainly exposed to epididymal cauda fluid and its specific proteins i.e. various lipocalins, including the fertility-related prostaglandin D synthase; than to prostatic and initial vesicular gland secretions. Pl-spermatozoa are hence exposed to less bicarbonate, zinc or fructose and mainly to PSP-I spermadhesin than if they were in the rest of the SRF and the post-SRF (P2). Since the PI-SP is less destabilizing for sperm membrane and chromatin, P1-spermatozoa sustain most in vitro procedures, including cryopreservaiion, the best. Moreover, ejaculated firstly, the Pl-spermatozoa seem also those deposited by the boar as a vanguard cohort, thus becoming overrepresented in the oviductal sperm reservoir (SR). This vanguard SR-entry occurs before the endometrial signalling of SP components (as PSP-VPSP-I and cytokines) and causes a massive influx of the innate defensive PMNs to cleanse the uterus from eventual pathogens, superfluous spermatozoa, and the allogeneic SP. The SP also conditions the mucosal immunity of the female genital tract, to tolerate the SR-spermatozoa and the semi-allogeneic conceptus. These in vivo gathered data can be extrapolated into procedures for handling boar spermatozoa in vitro for AI and other biotechnologies, including simplified cryopreservation. 39
PP2 MOLECULAR KINETICS OF PROTEINS AT THE SURFACE OF PORCINE SPERM BEFORE AND DURING FERTILIZATION P.S. Tsai and B.M. Gadella* Department of Biochemistry and Cell Biology, Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2 3584 CM Utrecht, The Netherlands E-mail : B.M.Gadella @uu.nl xPresenting author Fertilization is a decisive moment in life and enables the combination of the DNA from two gametes to ultimately form a new organism. The sperm surface, especially the head area, has distinguishable subdomains that are involved in distinct fertilization pro""*i"s. It is known that the sperm head surface undergoes constant remodelling during epididymal maturation and migration in the male and female genital tract. But intriguingly, the identity, origin and spatial ordering of proteins at the sperm surface that are involved in mammaú un f"rtilitution are essentially unknown. This review deals with sperm surface protein modifications that are under somatic cell control. As soon as the sperm is released from the seminiferous tubules it is subjected to these modifications. These surface reorganisations continue until the sperm reside in the fallopian tube where they meet the oocyte and may fenilize it. Most likely, a selective process allows only functionally mature and intact sperm to optimally interact aná fertilize the oocyte' Recent data suggest that even the perivitelline fluid is involved in sperm surface remodelling as it contains factors which could facilitate the first penetrating sperm to fertilize the oocyte. In this contribution, the kinetics of proteins at the sperm surface wili be overviewed. Better understanding of this would help to design strategies to improve male fertility or to devise novel contraceptives. 4l
Page 2 and 3: ø fHç VIII'h International Confer
Page 4 and 5: WELCOME FROM THE INTERNATIOIYAL ORG
Page 6: ___--:| WELCOME FROM THE DEPARTMENT
Page 9 and 10: SPONSORS Platinum Ø Wr"ffil"^Anima
Page 11 and 12: GENERAL INFORMATION
Page 13 and 14: MAP OF BANFF CENTRE rl+¡.f,¡lla @
Page 15 and 16: CONFERENCE PROGRAM suNDAY, MAY 31.t
Page 17 and 18: l5:00 - 15:30 General Discussion Br
Page 19 and 20: I ) ) ) ) ) ) ) ) ) I I I l3:30 - 1
Page 21 and 22: Breakout Session #6 "state-of-the A
Page 23 and 24: TRANSCRIPTIONAL, POST.TRANSCRIPTION
Page 25 and 26: ESTROGBN AND INTERLEUKIN-IP RBGULAT
Page 27 and 28: COMPARISON OF NUCLEOCOUNTER SP1OO A
Page 29 and 30: ISOLATION OF PORCINE EMBRYONIC STEM
Page 31 and 32: PROSTAGLANDIN ASSOCIATED WITH OXYTO
Page 33: ABSTRACTS OF INVITED PAPERS 37
Page 37 and 38: PP4 RECENT ADVANCES IN BOAR SEMEN C
Page 39 and 40: PP6 THE ROLE OF GENE DISCOVERY, QTL
Page 41 and 42: PP8 NUTRITIONAL AND LACTATIONAL EFF
Page 43 and 44: I I I I I PPlO I I INTRA.FOLLICULAR
Page 45 and 46: PP12 OVARIAN RESPONSES TO LACTATION
Page 47 and 48: PP1.4 PRENATAL PROGRAMMING OF POSTN
Page 49 and 50: PP16 FUNCTIONAL GENOMIC APPROACHES
Page 51 and 52: PP18 GROWTH, BODY STATE, AND BREEDI
Page 53 and 54: PP2O ANTILUTEOLYTIC MECHANISMS AND
Page 55 and 56: ABSTRACTS OF SHORT ORAL PRESENTATIO
Page 57 and 58: cells detected in both medium A and
Page 59 and 60: expected during production of semen
Page 61 and 62: 7.1-fold), TNF-u (AH: 1.9-fold) and
Page 63 and 64: colloids respectively, there was no
Page 65 and 66: for total teat number on SSC5 (57 c
Page 67 and 68: and interact with various receptors
Page 69 and 70: at weaning, follicle size at the st
Page 71 and 72: day 1d of weaning (d 0). Sow had no
Page 73 and 74: Table 1: Reproductive Characteristi
Page 75 and 76: stromal and glandular cells (Soloff
Page 77 and 78: TABLE 1: Differentially expressed p
Page 79 and 80: inding protein 1 (LIMAI) and a hypo
Page 81 and 82: Table 1: Germ cell enrichment by di
Page 83 and 84: Overall, the expression of Nanog, O
Page 85 and 86:
GT treatment may reflect the very s
Page 87 and 88:
Table 1: Untreated control sow inse
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Table 1: Number and percent of gilt
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SLC2A2 mRNA was most abundant in co
Page 93 and 94:
2st-6 THE EFFECT OF TIMING OF hCG A
Page 95 and 96:
251-8 PROTECTIVE EFFECT OF PSP.VII
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251-10 RELATION BETWEEN NUMBER OF S
Page 99 and 100:
25t-12 EVALUATION OF BOAR SEMEN FER
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25t-14 COMPARISON OF NUCLEOCOUNTER
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251-16 EFFECT OF BOAR SEMINAL PLASM
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251-18 CAN POST.THAW SPERM SURVIVAL
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251-20 INTRA.CERVICAL PIG AI WITH T
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252-tt BIRTH WEIGHT IMPLICATIONS FO
Page 111 and 112:
2s2-13 DIFFERENTIAL EXPRESSION AND
Page 113 and 114:
252-15 DIFFERENTIAL micToRNA EXPRES
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252-17 EXPRESSION OF HOXA1O IN BARL
Page 117 and 118:
2s2-19 EFFECT OF INTRAUTERINE CRO\r
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252-21 DEVELOPMENT OF NEONATAL PIGL
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)
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)
Page 125 and 126:
252-27 ESTROGEN RECEPTORS COLOCALIZ
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)0.05). These results were not sign
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252-31 EFFECT OF EMPTY UTERINE SPAC
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253-3 EFFECT OF SYNCHRONIZING OVULA
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253-7 EFFECT OF EARLY LIFE STRESSOR
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2s3-9 RELEVANCE OF LOCAL PROGESTERO
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253-11 CONJUGATED LINOLEIC ACIDS (C
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253-13 FACTORS ASSOCIATED WITH REDU
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253-15 TIMED INSEMINATION FOLLOWING
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2s3-17 ULTRASONOGRAPHIC COUNTING OF
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253-19 GENISTEIN ALTBR THE RELEASE
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253-21 DIETARY INTAKE DT]RING EARLY
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2s3-23 IMPROVING FERTILITY AND SAVI
Page 151 and 152:
2s3-2s EFFECTS OF DAY OF FARROWING
Page 153 and 154:
253-27 EFFECT OF CHRONOLOGICAL AND
Page 155 and 156:
253-29 EFFECT OF SUPPLEMENTING GILT
Page 157 and 158:
253-31 REPRODUCTIVE RESPONSES OF SO
Page 159 and 160:
G Gabilondo, D.,137 Gadella, 8.,47
Page 161:
V Valette,8.,737 Vøllet, J., 71, 1
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