Untitled - Pork CRC

PP16
FUNCTIONAL GENOMIC APPROACHES FOR THE STUDY OF
FETAL/PLACENTAL DEVELOPMBNT IN SWINE WITH SPECIAL EMPHASIS ON
IMPRINTBD GENES
S.R. Bischoffl'3, S. Tsair'3, N. Hardisont'0, A.A.Motsinger-Reif,g.R. Freking2, and J.A.
Piedrahita *r'3
rDepartment of Molecular Biomedical Sciences, College of Veterinary Medicine, North
Carolina State University, Raleigh, NC, USA; 2 USDA, ARS, U.S. Meat Animal Research
Center, Clay Center, NE 68933 -0|66,USA; 3 Center for Comparative Medicine and
Translational Research, North Carolina State University, Raleigh, NC, USA;a Bioinformatics
Research Center, Department of Statistics, North Carolina State University, Raleigh, NC 27695-
7566, USA
E-mail : jorge_piedrahita @ ncsu. edu
*Presenting author
This chapter describes the application of functional genomic approaches to the study of
imprinted genes in swine. While there are varied definitions of "functional genomics", in general
they focus on the application of DNA microarays, single nucleotide polymorphism (SNP)
affays, and other high coverage genomic analyses, and their combination with downsÍeam
methods of gene modification such as silencing RNA (siRNA) and viral and non-viral
transfection. Between the initial data acquisition and the actual genetic manipulation of the
system lies bioinformatics, where massive amounts of data are analyzed to extract meaningful
information. This area is in constant flux with an increased emphasis on detection of affected
pathways and processes rather than generation of simple affected gene lists. We will expand on
each of these points and describe how we have used these technologies for the study of
imprinted genes in swine. First we will introduce the biological question to provide context for
the discussion of the functional genomic approaches and the types of information they generate.
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