Confidential - Plant Research International - Wageningen UR
Confidential - Plant Research International - Wageningen UR
Confidential - Plant Research International - Wageningen UR
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EU-FISHOV: Personnel<br />
Catarina Matos<br />
Katinka van Roij (until March 1 st 2003)<br />
Gerard Rouwendal (from January 1 st 2003)<br />
Harm Wiegersma<br />
Shauna Hettinga<br />
Dirk Bosch<br />
Dion Florack<br />
<strong>Confidential</strong>
Summary LTB fusion proteins<br />
The larger the protein added to LTB, the lesser expression<br />
level:<br />
Steric hindrance or interference with pentamerization<br />
Solutions:<br />
Chimeric vaccines<br />
Use of LTA part<br />
Patent Application in progress<br />
<strong>Confidential</strong>
Summary N-glycosylation<br />
Mammals<br />
Humans<br />
( )<br />
( )<br />
<strong>Plant</strong>s<br />
General:<br />
N-acetylglucosamine<br />
Mannose<br />
Mammalian specific:<br />
Galactose<br />
Fucose (1,6)<br />
Sialic acid<br />
<strong>Plant</strong> specific:<br />
Xylose<br />
Fucose (1,3)<br />
<strong>Confidential</strong>
N-glycosylation<br />
Humanizing plant N-glycosylation manufacturing purposes<br />
Patent application: Galactosylation in plants<br />
Patent application: Bisected N-glycans in plants<br />
Patent application: Galactose and less Xylose and Fucose<br />
“Strategic Alliance” N-glycosylation with Dow Chem. (2002)<br />
Contract manufacturing (plant) biopharmaceuticals by Dow<br />
Focuss on therapeutic glycoproteins (human use)<br />
<strong>Confidential</strong>
N-glycosylation<br />
Dow: active research into edible plant-derived vaccines<br />
Focuss: livestock, no aquaculture (yet)<br />
Strong IP position plant-derived edible vaccines and<br />
manufacturing<br />
Future manufacturing through Dow<br />
<strong>Confidential</strong>
Questions remaining, choices to be made<br />
Bulk material: grow on request, when needed<br />
Licenses needed for GMO plant<br />
SVCV G protein derivative Where delete Tm<br />
Request for anti-SVCV G Ab<br />
What fusions to make: LTB-vhsv G/GTm; LTB-svcv G/<br />
GTm; HRP-vhsv/svcv G/ GTm<br />
<strong>Confidential</strong>
FISHOV website and advertising<br />
<strong>UR</strong>L: www.fishov.org<br />
Design, layout, who<br />
Proposal: public acc. website with project information, links<br />
to participant organizations and email contacts, “project<br />
members only”<br />
Examples: www.plant.wageningenur.nl/default.aspsection=projects<br />
Ideas<br />
<strong>Confidential</strong>
Consortium Agreement<br />
No progress to report<br />
Question: do we need it<br />
<strong>Confidential</strong>
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