Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Binding Complex on Capture Strip<br />
1) Wash capture strips on the platewasher using<br />
the “3X Wash” program.<br />
2) Using an 8-channel pipetter, add 30 µL of<br />
each reaction to a well of the capture strips.<br />
30 µL<br />
3) Place the rack of capture strips back on the<br />
plateshaker and shake for 30 minutes.<br />
Prepare Capture Strips for PCR amplification<br />
1) Wash capture strips on the platewasher using<br />
the “5x Wash” program. The series of washes<br />
and soaks takes 15 minutes.<br />
2) Thaw primer probe (white cap) and add 40 µL<br />
primer/probe, 160 µL of water (DNase, RNase<br />
free) and 200 µL of Universal Master Mix per<br />
capture strip used to a glass test tube. Mix<br />
gently.<br />
+ Master + H 2 O<br />
Mix<br />
3) Add the primer/probe + Master Mix solution<br />
to a pipetting trough, and using an 8-channel<br />
pipetter, add 40 µL to each well of the capture<br />
strips.<br />
40 µL<br />
4) Seal the wells with an adhesive optical cover,<br />
cover with 2 compression pads and insert the<br />
strips into the thermocylcer of the PCR<br />
instrument.
Change language