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L. Procept Quick Reference<br />
Platewasher Set Up<br />
1) Dilute 40 mL of PCR wash concentrate with<br />
960 mL of deionized water.<br />
2) Add the wash solution to the reservoir of the<br />
plate washer.<br />
Wash<br />
Buffer<br />
+ H 2 O<br />
3) Prime plate washer with PCR wash solution.<br />
Prepare Capture Strips<br />
1) Wash the capture strips using the “3X Wash”<br />
program.<br />
2) Thaw capture reagent (red cap).<br />
3) In a glass test tube, dilute 40 µL of Capture<br />
Reagent concentrate with 600 µL of Assay<br />
Buffer for each capture strip used.<br />
+<br />
Assay<br />
Buffer<br />
4) Using an 8-channel pipetter, add 50 µL of the<br />
Capture Reagent to each well of the capture<br />
strips.<br />
50 µL<br />
5) Place capture strips on a plateshaker and shake<br />
for 60-90 minutes.