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RFP an ntibody [3 3F5] 1 - ChromoTek
RFP an ntibody [3 3F5] 1 - ChromoTek
RFP an ntibody [3 3F5] 1 - ChromoTek
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Tested<br />
Applications<br />
<strong>an</strong>d<br />
Recommended<br />
Dilutions<br />
Western Blot:<br />
Mouse <strong>RFP</strong> <strong>an</strong>tibody <strong>3F5</strong> is highly<br />
specific to<br />
<strong>RFP</strong>s <strong>an</strong>d shows no<br />
crossreactivity to GFP<br />
or <strong>an</strong>y other<br />
cellular protein.<br />
primary <strong>an</strong>tibody:<br />
1/1000 <strong>RFP</strong> <strong>an</strong>tibody <strong>3F5</strong><br />
incubationn for 1 h at RT<br />
secondary<br />
<strong>an</strong>tibody:<br />
1/500 <strong>an</strong>ti-mouse-Alexa647<br />
incubationn for 1 h at RT<br />
Sensitivity r<strong>an</strong>ge of mouse <strong>RFP</strong> <strong>an</strong>tibody <strong>3F5</strong> goes down to 10 ng of<br />
purified <strong>RFP</strong> protein. <strong>3F5</strong> detects a b<strong>an</strong>d of approximately 27 kDa<br />
(predicted molecular weight <strong>RFP</strong>: 27 kDa).<br />
primary <strong>an</strong>tibody:<br />
1/2000 red <strong>an</strong>tibody <strong>3F5</strong><br />
incubation for 1 h at RT<br />
with 3% milk in TPBS<br />
secondary <strong>an</strong>tibody:<br />
1/ 5000 <strong>an</strong>ti-mouse-<br />
Alexa647<br />
incubation for 1 h at RT<br />
with 3% milk in TPBS<br />
Not yet tested in other applications. Optimal dilutions/concentrations should be<br />
determined by the end user.<br />
Only for<br />
research applications, not for diagnostic or therapeutic<br />
use.<br />
<strong>RFP</strong> <strong>an</strong>tibody [<strong>3F5</strong>] (Mouse monoclonal)<br />
2<br />
Version 2011-07-05
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