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RFP an ntibody [3 3F5] 1 - ChromoTek
RFP an ntibody [3 3F5] 1 - ChromoTek
RFP an ntibody [3 3F5] 1 - ChromoTek
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<strong>RFP</strong> <strong>an</strong>tibody [<strong>3F5</strong>]<br />
Description<br />
Product<br />
Type<br />
Specificity<br />
Isotype<br />
Relev<strong>an</strong>ce<br />
Form<br />
Size<br />
Storage<br />
Buffer<br />
Concentration<br />
Storage<br />
instructions<br />
Mouse monoclonal [<strong>3F5</strong>]<br />
to Red Fluorescent Proteins.<br />
For biochemical <strong>an</strong>alysiss of <strong>RFP</strong>-tagged fusion proteins.<br />
Primary <strong>an</strong>tibody<br />
<strong>3F5</strong> recognizes red fluorescent proteins derived from DsRed <strong>an</strong>d<br />
<strong>RFP</strong>-tagged fusion proteins.<br />
IgG2a<br />
Fluorescent proteins (FP) are powerful tools to study protein<br />
localization <strong>an</strong>d dynamics in living cells In addition to the prominent<br />
green fluorescent protein<br />
(GFP) from<br />
Aequorea victoria, a red<br />
fluorescent protein (DsRed) was discovered in the coral Discosoma<br />
sp. Mutagenesis of the red progenitor have resulted in several<br />
monomeric red FPs (m<strong>RFP</strong>1, mCherry, mOr<strong>an</strong>ge, mPlum, etc)<br />
which become attractive tools for a number of applications. For<br />
example the<br />
combinationn of green <strong>an</strong>d red fusion<br />
proteins allows<br />
multicolor tracking as well as protein-protein <strong>an</strong>tibody<br />
(MAb) against monomeric<br />
<strong>RFP</strong>s, designated as <strong>RFP</strong> <strong>an</strong>tibody [<strong>3F5</strong>]. The <strong>3F5</strong> MAb is<br />
interaction studies in<br />
living cells.<br />
Use this mouse monoclonal<br />
characterized by high affinity <strong>an</strong>d specificity against the monomeric<br />
DsRed derivatives <strong>an</strong>d corresponding fusion proteins It is suitable<br />
for immunoblotting assays.<br />
Liquid; hybridoma supernat<strong>an</strong>t. The hybridoma was cultured in<br />
protein free medium. Tissue culture supernat<strong>an</strong>t<br />
was concentrated<br />
by centrifugation using a 30 kD exclusion membr<strong>an</strong>e to <strong>an</strong> <strong>an</strong>tibody<br />
concentration of 1 mg/ml.<br />
100 µg<br />
Preservative: 0.01% Sodium Azide<br />
Material safety datasheet (MSDS) for this product:<br />
Sodium Azide MSDS<br />
1 mg/ml<br />
Store at +4° C.<br />
<strong>RFP</strong> <strong>an</strong>tibody [<strong>3F5</strong>] (Mouse monoclonal)<br />
1<br />
Version 2011-07-05
Tested<br />
Applications<br />
<strong>an</strong>d<br />
Recommended<br />
Dilutions<br />
Western Blot:<br />
Mouse <strong>RFP</strong> <strong>an</strong>tibody <strong>3F5</strong> is highly<br />
specific to<br />
<strong>RFP</strong>s <strong>an</strong>d shows no<br />
crossreactivity to GFP<br />
or <strong>an</strong>y other<br />
cellular protein.<br />
primary <strong>an</strong>tibody:<br />
1/1000 <strong>RFP</strong> <strong>an</strong>tibody <strong>3F5</strong><br />
incubationn for 1 h at RT<br />
secondary<br />
<strong>an</strong>tibody:<br />
1/500 <strong>an</strong>ti-mouse-Alexa647<br />
incubationn for 1 h at RT<br />
Sensitivity r<strong>an</strong>ge of mouse <strong>RFP</strong> <strong>an</strong>tibody <strong>3F5</strong> goes down to 10 ng of<br />
purified <strong>RFP</strong> protein. <strong>3F5</strong> detects a b<strong>an</strong>d of approximately 27 kDa<br />
(predicted molecular weight <strong>RFP</strong>: 27 kDa).<br />
primary <strong>an</strong>tibody:<br />
1/2000 red <strong>an</strong>tibody <strong>3F5</strong><br />
incubation for 1 h at RT<br />
with 3% milk in TPBS<br />
secondary <strong>an</strong>tibody:<br />
1/ 5000 <strong>an</strong>ti-mouse-<br />
Alexa647<br />
incubation for 1 h at RT<br />
with 3% milk in TPBS<br />
Not yet tested in other applications. Optimal dilutions/concentrations should be<br />
determined by the end user.<br />
Only for<br />
research applications, not for diagnostic or therapeutic<br />
use.<br />
<strong>RFP</strong> <strong>an</strong>tibody [<strong>3F5</strong>] (Mouse monoclonal)<br />
2<br />
Version 2011-07-05
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