SC400 Colorimeter COD - Orbeco-Hellige

Photometer-System
SC400 Colorimeter
SiO 2
COD
DE
GB
FR
IT
ES
Bedienungsanleitung
Seite 3–17
Instruction Manual
Page 19–33
Mode d'emploi
Page 35–49
Instruction Manual
Istruzioni d'uso
Pagina 51–65
Instrucciones
Página 67–81

Requirements
Method: Oxygen Demand, Chemical
Method Description: The sample is heated at 150 o C for two hours in the presence of
a potassium dichromate, which is a strong oxidizing agent. Organic compounds in the
sample are oxidized, reducing the dichromate ion (Cr 2
O 7
2–
) to the green chromic ion (Cr3 +) .
In the Low Range (LR) Test (0-150 mg/L), the amount of remaining Cr 6+ is measured; In the
Mid Range (MR) (0-1,500 mg/L) or (HR) (0-15,000 mg/L), the amount of Cr 3+ produced is
measured. The results are measured as the amount of oxygen (O2) consumed per liter of
water sample. The LR and MR test is displayed in mg/L while the HR test shows g/L.
The COD reagent contains both mercury and silver—the mercury controls chloride
interferences and the silver functions as a catalyst to speed the reaction.
Required Instruments:
SC400 Colorimeter for COD (P/N: SC400-00)
TR125 Thermoreactor (P/N: TR12500)
Required Accessories: 16 mm Tube Adapter (P/N: L19802220)
Required Reagents:
Range: Reagent Description: Reagent P/N:
0-150 mg/L Low Range COD Tube Test, 25 Tests TT20720
OR Low Range COD Tube Test, 150 Tests TT20725
0-1500 mg/L Mid Range COD Tube Test, 25 Tests TT20721
OR Mid Range COD Tube Test, 150 Tests TT20726
0-15000 mg/L High Range COD Tube Test, 25 Tests TT20722
OR High Range COD Tube Test, 150 Tests TT20727
Alternate Reagents:
For non-reporting purposes, our complete line of Mercury-Free COD reagents is available.
These reagents follow the same procedure and calibrations pre-programmed in the
SC400-00. These reagents are not acceptable for USEPA reporting purposes.
Range: Reagent Description: Reagent P/N:
0-150 mg/L Low Range COD Tube Test, Mercury-Free, 25 Tests TT20710
0-1500 mg/L Mid Range COD Tube Test, Mercury-Free, 25 Tests TT20711
Mid Range COD Tube Test, Mercury-Free,150 Tests TT20712
0-15000 mg/L High Range COD Tube Test, Mercury-Free, 25 Tests TT20716
2 SC400-00, COD 03/2009

Contents
• General Notes......................................................4
Method notes .......................................................4
Chemical method notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Battery change . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
• Special Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Backlight ...........................................................5
Recall of stored data ..................................................5
Measuring the absorbance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
• Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Tube Test Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Preparing Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Guidelines for colorimetric measurement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
• Menu Options......................................................8
Menu selections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Recall of stored data ..................................................8
Setting date and time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
• Calibration Mode ...................................................9
User calibration ......................................................9
Recommended calibration values . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Factory calibration reset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
• Technical Data.....................................................11
Operating messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Error codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
SC400-00, COD 03/2009
3

General Notes
Method notes
• Prior to measurement ensure that the sample is suitable for analysis (no major interferences,
such as chloride-see below) and does not require any preparation i.e. pH adjustment,
filtration etc.
• Reagents are designed for use in chemical analysis only and should be kept out of the
reach of children.
• Ensure proper disposal of reagent solutions.
• Material Safety Data Sheets: available on our website (www.orbeco.com) or upon
request.
Chemical method notes:
Method:
The organic material present in the sample is oxidizing by a standard amount of a potassium
dichromate oxidized mixture. After oxidation is complete, the excess of this reagent is
measured photometrically.
Application:
Samples can be measured if the chloride content does not exceed 1,000 mg/L (LR/MR) or
10,000 mg/L (HR).
In rare cases, compounds contained in the water cannot be oxidized adequately. This results
in low results compared with the reference method.
Different methods of sampling, preparation of the sample itself and the time elapsed between
taking the sample and analysis can all affect the obtained results.
CAUTION
The accuracy of the instrument is only valid if the instrument is used in an
environment with controlled electromagnetic disturbances according to DIN 61326.
Wireless devices, e.g. wireless phones, must not be used near the instrument.
Battery change
4 SC400-00, COD 03/2009

Special Functions
Display backlight
!
Press the [!] key to turn the display backlight on or off. The backlight
is turned off automatically during the measurement.
Recall of stored data
!
If the instrument is turned on, press the [!] key for more than
4 seconds to access the recall menu.
Measuring the absorbance
AbS
This instrument ordinarily measures using two wavelengths. In the
AbS mode, it is possible to measure absorbance at a specified
wavelength.
Abs1:
Abs2:
= 430 nm
= 605 nm
SC400-00, COD 03/2009
5

Method
COD Tube Test Reagents
Select the appropriate vial for the desired range:
LR-Range, 25 Tests: 0 – 150 mg/L P/N TT20720
MR-Range, 25 Tests: 0 – 1,500 mg/L P/N TT20721
HR-Range, 25 Tests: 0 – 15,000 mg/L P/N TT20722
Preparing the Sample
1. Open a reaction vial with a white cap and add the specified volume.
(Ensure that appropriate personnel safety equipment is used.)
LR-/MR: 2 ml water sample
HR: 0.2 ml water sample
2. Prepare a blank (Note 1) by using deionized water (TOC-free) instead of the sample
(LR/MR: 2 ml, Hr: 0.2 ml).
3. Replace the cap tightly. Invert the vial gently several times to mix the contents (The
vial will become hot during mixing!) and digest the vials for 120 minutes in the
reactor at a temperature of 302°F (150°C).
4. Remove the vials from the reactor and allow them to cool down to 60°C or less. Mix
the contents by inverting each vial several times while still warm. Then allow the vials to
cool to ambient temperature before measuring.
Measurement
On
Off
Lr
Mode
RANGE
Zero
Test
RANGE
0.0.0
1. Insert the 16 mm adapter into the sample chamber by aligning the
arrow on the adapter with the arrow on the instrument. Be sure that
the adapter seats completely into the sample chamber.
2. Turn on the unit using the [ON/OFF] key.
The display shows the following:
3. Select the required test using the [MODE] key:
LR (low range) MR (mid range) HR (high range) AbS1
(Absorbance 1) AbS2 (Absorbance 2) LR ......... (Scroll)
The display shows the following:
4. Place the blank in the adapter (Note 1–4) making sure that the
marks ı are aligned. Blanks are specially prepared for each
individual test range.
5. Press the [ZERO/TEST] key.
The "Range" symbol flashes for approximately 8 seconds.
The display shows the following: 0.0.0
6. After zeroing, remove the vial from the adapter.
6 SC400-00, COD 03/2009

Method
Zero
Test
RANGE
RESULT
1. Press the [ZERO/TEST] key.
The "Range" symbol flashes for approx. 3 seconds.
The result appears in the display.
The result is saved automatically.
LR-/MR-range: in mg/L
HR-range: in g/L
(this unit is shown by an alternating display of the
reading and g : L)
Accuracy: ± 3.5 % (full scale)
Zero
Test
Repeating the test:
Press the [ZERO/TEST] key again.
Mode
New zero calibration:
Press the [MODE] key until the desired range symbol appears in the
display again.
Guidelines for colorimetric measurements
1.
2.
3.
4.
5.
6.
7.
8.
Run samples and blanks from the same batch of vials.
The blank is stable when stored in the dark and can be used for further measurements
with vials from the same batch.
Don't place hot vials in the adapter. Allow the vials to cool to room temperature for
minimum 45 minutes. It is recommended to let the vials to cool overnight.
Suspended solids in the vial lead to incorrect measurements. For this reason, it is important
to place the vials carefully in the adapter. The precipitant at the bottom of the sample
should not be suspended.
Clean the outside of the vials with a towel to remove fingerprints and other marks.
Avoid spilling water in the sample chamber. If water should spill into the chamber, absorb
it with a soft cloth or tissue.
Contamination of the optics (LED and photo-sensor) in the sample chamber will result in
incorrect measurements. The windows in of the sample chamber must be checked at
regular intervals and cleaned as necessary. Use a moistened cloth or cotton swab for
cleaning purposes.
Large temperature differences between the colorimeter and ambient conditions can lead
to incorrect measurements, or build-up of condensation around the optics of the vial.
To avoid errors caused by stray light do not use the instrument in bright sunlight.
SC400-00, COD 03/2009
7

Menu Options
Store
Time
Mode
On
Off
!
Mode
Date
1
2
3 4
Cal
Menu selections
1. Press the [MODE] key and hold.
2. Turn on the unit using the [ON/OFF] key.
Allow the 3 decimal points to be displayed before releasing the
[MODE] key.
The [!] key allows for selection of the following menu points:
1 recall stored data
2 3 setting the date and time
4 user calibration
The selected menu is indicated by an arrow in the display.
Confirm the selection with the [MODE] key.
1 Recall of stored data
Zero
Test
Mode
!
The colorimeter shows the last 16 data sets in the following format
(automatically proceeds every 3 seconds until result is displayed):
Number n xx (xx: 16...1)
Year YYYY (i.e. 2008)
Date mm.dd (monthmonth:dayday)
Time hh:mm (hourhour:minuteminute)
Test Method
Result x,xx
The [ZERO/TEST] key repeats the current data set.
The [MODE] key scrolls through all stored data sets.
Quit the menu by pressing [!] key.
Mode
SET
DATE
YYYY
(2 sec.)
Mode
Zero
Test
!
2
3
Setting date and time (24-hour-format)
After confirming the selection with the [MODE] key the value to be
edited will be shown for 2 sec.
The setting starts with the year (YYYY) followed by the actual value
to be edited. Same applies for month (mm), day (dd), hour (hh) and
minutes (mm). Set the minutes first in steps of 10, press the [!] key to
continue setting the minutes in steps of 1.
Increase the value by pressing the [MODE] key.
Decrease the value by pressing [ZERO/TEST] key.
Proceed to the next value to be edited by pressing [!] key.
After setting the minutes and pressing the [!] key the display will show
“IS SET” and instrument returns to the measurement mode.
Note: If the battery is removed for more than one minute the date
and time menu starts automatically when the photometer is next
turned on.
8 SC400-00, COD 03/2009

Calibration Mode
4 User calibration
cAL
CAL
CAL
Lr
Zero
Test
RANGE
0.0.0
CAL
Zero
Test
RANGE
RESULT
CAL
Mode
Zero
Test
CAL
RESULT + x
On
Off
: :
Note:
User calibration (Display in calibration mode)
Factory calibration (Display in calibration mode)
After confirming the selection with the [MODE] key the instrument
will show CAL/Lr.
Scroll through ranges using the [MODE] key.
Perform zero calibration (see "Operation").
Press the [ZERO/TEST] key.
The "Range" symbol flashes for approx. 8 seconds.
The display shows the following in alternating mode:
Perform calibration with a standard of known concentration (see
"Operation").
Press the [ZERO/TEST] key.
The "Range" symbol flashes for approx. 3 seconds.
The result is shown in the display, alternating with CAL.
If the reading corresponds with the value of the calibration standard
(within the specified tolerance), exit calibration mode by pressing the
[ON/OFF] key.
Changing the displayed value:
Pressing the [MODE] key once increases the displayed value by
1 digit.
Pressing the [ZERO/TEST] key once decreases the displayed value by
1 digit.
Press the corresponding key until the reading equals the value of the
calibration standard.
By pressing the [ON/OFF] key, the new correction factor is calculated
and stored in the user calibration software.
Confirmation of calibration (3 seconds).
Recommended calibration values
LR: 100 mg/L O 2
MR: 500 mg/L O 2
HR: The instrument calibration in the range MR
is automatically taken also for HR.
SC400-00, COD 03/2009
9

Calibration Mode
Factory calibration reset
Resetting the user calibration to the original factory calibration will
reset all methods and ranges.
Store
Date
RESULT
Time
Cal
Zero
Mode
Test
On
Off
A user calibrated method is indicated by an arrow while the test result
is displayed.
To reset the calibration press both the [MODE] and [ZERO/TEST] key
and hold.
Turn on the unit using the [ON/OFF] key.
Release the [MODE] and [ZERO/TEST] keys after approx. 1 second.
The following messages will appear in turn on the display:
SEL
CAL
The factory setting is active.
(SEL stands for Select)
or:
SEL
cAL
Calibration has been set by the user.
(If the user calibration is to be retained, turn the unit off using the
[ON/OFF] key).
Mode
SEL
CAL
On
Off
Calibration is reset to the factory setting by pressing the [MODE] key.
The following messages will appear in turn on the display:
Turn off the unit using the [ON/OFF] key.
10 SC400-00, COD 03/2009

Technical Data – Operating Messages
Light source:
Battery:
Auto-OFF:
Technical Data
Lr-Range: LED, Filter ( = 430 nm)
Mr-/Hr-Range: LED ( = 605 nm)
9 V-block battery (Life approx. 600 tests),
without display light.
Automatically turns off 7 minutes after last keypress
Ambient conditions: 5–40°C (41-104 o F)
30–90% rel. humidity (non-condensing).
CE:
Certificate for Declaration of CE-Conformity
at www.orbeco.com
To ensure the maximum accuracy of test results, always use the reagent systems
supplied by the instrument manufacturer.
Operating messages
Hı
Lo
Measuring range exceeded or excessive turbidity.
Result below the lowest limit of the measuring range.
Replace 9 V battery, no further tests possible.
Error codes
E 01
E 10
E 20/E 21
E 22
Light absorption too great. Reasons: e.g. dirty optics.
Calibration factor "out of range"
Too much light reaching the detector
Battery capacity was too low during measurement. Change battery.
E 70
E 71
E 72
E 73
Lr:
Lr:
Mr:
Mr:
Factory calibration incorrect / erase
User calibration incorrect / erase
Factory calibration incorrect / erase
User calibration incorrect / erase
For assistance with Error Codes or other technical issues, please contact Customer Service.
(Phone: 941.756.6410 or Toll-free U.S. 800.922.5242)
SC400-00, COD 03/2009
11

Technical changes without notice
FOR TECHNICAL ASSISTANCE, PRICE AND ORDERING
INFORMATION:
In the USA - Call 800-922-5242 or the distributor serving you.
Outside the USA: Call 941-756-6410 or the distributor serving you.
Web: www.orbeco.com
Email: service@orbeco.com
Fax: 941-727-9654
© Orbeco-Hellige, Inc, 2009. All rights reserved.
Printed in Germany. Updated March 2009, Edition 1