full issue - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and Pharmacy
Vol. 7 (1) 511-517 January 2013, ISSN 0973-8916 (Print), 2230-7303 (Online)
515
Table 2. Effect of plant growth regulators on direct adventitious shoot formation from cotyledon
explants of A. mangium.
Plant growth regulators Mean no. of shoots Mean length of
(μm) per explant shoots (cm)
BA
1.0 1.20 ± 0.80 f 0.99 ± 0.41 cd
2.0 3.87 ± 0.67 cde 2.32 ± 0.96 abcd
4.0 2.20 ± 0.96 ef 2.16 ± 0.91 abcd
6.0 1.20 ± 0.29 f 0.78 ± 0.29 d
8.0 0.80 ± 0.80 f 0.84 ± 0.31 cd
BA + NAA
1.0 + 0.5 4.12 ± 0.51 cde 2.69 ± 0.26 abc
1.0 + 1.0 3.25 ± 0.36 cde 1.65 ± 0.18 bcd
1.0 + 2.0 1.37 ± 0.32 f 1.21 ± 0.22 cd
2.0 + 0.5 7.25 ± 0.65 bc 3.25 ± 0.47 ab
2.0 + 1.0 10.88 ± 0.35 a 3.90 ± 0.23 a
2.0 + 2.0 3.08 ± 0.81 de 1.78 ± 0.56 bcd
4.0 + 0.5 6.75 ± 0.61 bcd 2.16 ± 0.32 abcd
4.0 + 1.0 8.37 ± 0.73 ab 1.72 ± 0.16 bcd
4.0 + 2.0 2.56 ± 0.75 f 1.52 ± 0.45 bcd
BA + IBA
1.0 + 0.5 3.12 ± 0.51 de 2.05 ± 0.64 bcd
1.0 + 1.0 2.37 ± 0.80 ef 1.60 ± 0.53 bcd
1.0 + 2.0 1.37 ± 0.67 f 1.20 ± 0.61 cd
2.0 + 0.5 3.62 ± 0.53 cde 2.14 ± 0.52 abcd
2.0 + 1.0 6.12 ± 0.81 bcd 2.16 ± 0.52 abcd
2.0 + 2.0 1.62 ± 0.44 f 1.14 ± 0.57 cd
4.0 + 0.5 3.87 ± 0.61 cde 2.13 ± 0.71 abcd
4.0 + 1.0 3.50 ± 0.73 cde 1.93 ± 0.73 bcd
4.0 + 2.0 1.12 ± 0.34 f 0.69 ± 0.46 d
Values represent means ± standard error of 15 explants per treatment in three repeated
experiments. The different letters within column show significant differences by ANOVA and
Duncan’s multiple range tests at p