full issue - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and Pharmacy
Vol. 7 (1) 518-526 January 2013, ISSN 0973-8916 (Print), 2230-7303 (Online)
525
of 48 h to study its effect on L-asparaginase
production. The maximum enzyme production
(5.6 U/ml) was observed with 1.2 (% w/v) as
shown in Fig. 7. Further increase in L-
asparaginase concentration resulted in the
decrement of enzyme production.
Discussions for isolation of Streptomyces
species strain: The strain grows well on most
of the media. The sporophores occurred as spiral
spore chains. The aerial mycelium developed
moderately to good on most of the media and is
gray in colour. The vegetative of the mycelium
white to gray is almost dependent on media. The
strain is non-chromogenic without any
characteristic diffusible pigment and it produces
soluble pigment. The strain H 2
S and Tyrosinase
reactions are positive. It showed moderate
diastatic activity and showed a good proteolytic
activity by hydrolyzing casein and starch. This
strain PW-A12 is capable of coagulating and
peptonizing the milk. It exhibits positive nitrate
reduction. It grows well at 37 o C. It tolerated the
pH levels 6.5-7.5 good growth. It exhibited good
growth on glucose, sucrose, Inositol, D-mannitol,
D-fructose, lactose, maltose and Galactose. It
showed poor to moderate growth on L-arabinose,
D-xylose and cellulose. It exhibited good
antibacterial activity. It exhibited good growth on
L-arginine, L-cysteine and potassium nitrate.
Moderate growth is on L-asparagine, L-histidine,
and L-valine containing medium. Poor growth is
on tyrosine containing medium.
Summary and Conclusion
Enzymes used for therapeutic purposes,
cover a wide range of disease and conditions
viz. inborn errors of metabolism, cancer, blood
clotting deficiency etc. L-Asparaginase
(E.C.3.5.1.1, L-Asparagin Amido hydrolase) used
alone or in combination with other drugs is finding
increased success in the management of
childhood lymphoblastic leukaemia. Keeping the
potential of this particular enzyme in cancer
treatment, the present work is planned with 5
different estuarine sediment samples, 20
Actinomycetes strains were isolated. Among
them 5 strains showing pink colour zones with
high intensity were selected for the production
of L-asparaginase. Out of 5 Actinomycetes
strains PW-A12, 4th strain exhibited maximum
L-asparaginase activity with a yield of 3.933 IU/
ml for 24 hrs Submerged Fermentation time.
Further, optimizations of parameters were
conducted with the strain PW-A12, by
consecutive evaluation method. Maximum
enzyme production with a yield of 5.73 IU/ml was
observed with 4% peptone at pH 7.5. Reduced
enzyme production was observed (5.6 IU/ml)
while using on Yeast extract concentration of 1.2
gm / 100 ml and at a pH of 7.0. After optimization,
the production of L-asparaginase increased by
1.45 times. The results of the present study
indicated a scope for exploring estuarine
Actinomycetes as a source for extracellular L-
asparaginase, an enzyme that has gained
industrial and pharmaceutical significance.
References
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2. Verma, N., Kumar, K., Kaur, G. and Anand
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3. Tek, C.B., Monica, S. and Nitya, N.S. (2007)
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Rao et al