CaribVET activities
CaribVET activities
CaribVET activities
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New development of molecular diagnostic at<br />
CIRAD France + Guadeloupe<br />
‣ Extraction critical:<br />
4 extraction kits/techniques tested<br />
(Qiagen, Rneasy, Trizol, Macherey-Nagel)<br />
‣ Real time PCR for gene M:<br />
Weybridge protocole<br />
Adiavet (internal avian gene control)<br />
Applied-biosystem (gene M + H5)<br />
‣ H5-H7 real time PCR<br />
Weybridge, Padova and CIRAD interlaboratory assay<br />
‣ Inter laboratory assay<br />
French reference laboratory for AI (AFSSA)<br />
‣ Choice of primers and probes in the Caribbean<br />
According to circulating strains: PANAFTOSA-CIRAD-Ames
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