...from 450 up to 800 nm - BIOTREND Chemikalien GmbH

analysis • Products for Life Sciences Research and Bioanalysis • Products for Life Sciences a
New Fluorescent Dyes
...from 450 up to 800 nm
2 0 1 1
CF TM 405M Dye, CF TM 488A Dye, CF TM 633 Dye, CF TM 640R Dye,
CF TM 660C & CF TM 660R Dye, CF TM 680 & CF TM 680R Dye, Near-IR CF TM Dyes
CF TM 405M Dye
A superior 405 nm-excitable blue fluorescent dye
Technical Summary:
Abs/Em Maxima: 408/452 nm
Extinction coefficient: 41,000
Molecular weight: ~ 503
Flow cytometry laser line: 405 nm
Microscopy laser line: 405 nm
Direct replacement for: Pacific Blue® dye, BD Horizon V450
Figure 1.
Absorption and
emission spectra
of CF TM 405M and
Pacific Blue® dye
conjugated to goat
anti-mouse IgG
in PBS.
Advantages
• More photostable than Pacific Blue® dye
• As bright as Pacific Blue® dye in the 450/50 blue channel
while having less spillover in the 525/50 green channel
• Highly water-soluble
CF405M is a blue fluorescent dye spectrally similar to Pacific
Blue® dye (Figure 1). CF405M is as bright as Pacific Blue® dye
but is significantly more photostable than the latter (Figures
2 and 4). An additional advantage of CF405M is that it has less
spillover in the 525/50 green channel than Pacific Blue® dye due
to narrower emission peak (Figure 1), making CF405M a superior
choice in multi-color detection applications. We also offer
CF405S, another 405 nm-excitable dye with an even shorter
emission wavelength (See CF405S Flyer for details).
A list of CF405M-based products are shown in Table 1.
A full selection of secondary antibodies, antibody labeling kits,
and other bioconjugates including phalloidins, annexin V and
α-bungarotoxin are also available for many CF dyes.
Visit the BIOTREND website: www.biotrend.com for details.
...distributed by:
BIOTREND Chemikalien GmbH
Im Technologiezentrum Köln
Eupener Str. 157
D-50933 Köln
Germany
Tel. + 49 221 9 49 83 20
Fax. + 49 221 9 49 83 25
jaeger@biotrend.com
www.biotrend.com
®

...CF TM 405M Dye
A superior 405 nm-excitable blue fluorescent dye
Ordering information
Figure 3.
Photostability of CF405M
and Pacific Blue dyes.
CF405M and Pacific Blue dye
solutions at the same concentration
were continuously
exposed to a mercury
arc lamp microscope with a
DAPI filter set. Images were
taken at every 5 seconds.
Fluorescence intensity was
normalized to t = 0.
Figure 2.
Jurkat cells were stained
with intracellular CD3
followed by CF405M
or Pacific Blue goat antimouse
IgG conjugates.
Fluorescence was analyzed
on a BD LSR II flow
cytometer equipped with
a 405 violet laser and
450/50 nm emission
detection.
The bars represent the
relative fluorescence
of the geometric means
of the population
of cells. Background
was determined by
secondary antibody
staining alone.
Table 1. CF405M Product List
Product Size Cat.No Price in €
CF405M-Labeled Secondary Antibody Conjugates
Goat Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20180 141,00
Goat Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL
(min x Human, Bovine, Horse, Rabbit, and Swine) 0.5 mL 20182 174,00
Other CF405M-Labeled Products
Goat Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20181 141,00
Annexin V, 50 mg/mL 0.5 mL 29009 272,00
CF405M Reactive Dyes and Labeling Kits
Streptavidin 1 mg 29033 141,00
CF405M maleimide 1 µmole 92021 212,00
CF405M succinimidyl ester 1 µmole 92111 228,00
CF405M SE protein labeling kit 3 labelings (for 1 mg protein each) 92212 228,00
CF405M SE microscale protein labeling kit 3 labelings (for 20-100 µg protein each) 92232 120,00
CF TM 488A Dye
A superior green dye to Alexa Fluor® 488
Technical Summary:
Abs/Em Maxima: 490/515 nm
Extinction coefficient: 70,000
Molecular weight: ~ 91
Flow cytometry laser line: 488 nm
Microscopy laser line: 488 nm
Direct replacement for: Alexa Fluor® 488, DyLight488,
FITC, FAM, Cy2
Advantages
• Yields biologically more specific antibody conjugates
and has less “spill-over” fluorescence in the red channel
than Alexa Fluor® 488
• Extremely photostable
• Highly water-soluble and pH-insensitive
CF488A is a green fluorescent dye optimally excitable by the
488 nm argon laser line. Under common detection conditions,
CF488A is at least as bright as Alexa Fluor® 488. However,
a major advantage of CF488A over Alexa Fluor® 488 is that
antibody conjugates prepared from the former are biologically
more specific. Alexa Fluor® 488 carries multiple negative
charges, which can significantly change the isoelectric point of
the proteins the dye labels and consequently alter the specificity
of the protein conjugates. CF488A, on the other hand, is minimally
charged. Thus, antibody conjugates prepared from the dye
ensure biological detection with high signal-to-noise ratio.
Another feature of CF488A is that the emission peak wavelength
is about 10 nm shorter than that of Alexa Fluor® 488 and
15 nm shorter than that of the traditional green dye FITC (or FAM).
The shorter wavelength of CF488A offers the advantage of less
fluorescence “spill-over” in the red channel in multi-color
detection applications.
A list of CF488A-based products are shown in Table 1.
A full selection of secondary antibodies, antibody labeling kits,
and other bioconjugates including phalloidins, annexin V and
α-bungarotoxin are also available for many CF dyes.
Please visit the BIOTREND website at www.biotrend.com
for details.

Figure 1.
Absorption and emission
spectra of CF TM 488A (blue),
Alexa Fluor® 488 (green)
and FITC (red) conjugated
to goat anti-mouse IgG
in PBS.
Figure 3.
Cryosections (6 µm) of human control heart sections
stained with anti-fibronectin followed by goat anti-rabbit
IgG labeled with CF488A, Cy2 and Alexa Fluor® 488,
respectively. Courtesy of Dr. Sawa Kostin at Max-Planck-
Institute für Herz- und Lungenforschung (W.G. Kerckhoff-
Institut) in Bad Nauheim (Hessen)-Germany.
Figure 2.
Jurkat cells were stained
with intracellular CD3 or
isotype control (BD Biosciences)
followed by
goat anti-mouse IgG
conjugates from the
manufacturer’s shown
above. The inset graph
depicts the median fluorescence
of the population
as analyzed on a
Beckman Coulter FC-500.
The histogram depicts
an overlay of CD3-stained
cells with CF488A (green),
AlexaFluor488 (blue),
DyLight488 Pierce
(purple) and DyLight488
KPL (red) as analyzed
on a BD FACS Calibur in
the FL1 channel.
Ordering information
Table 1. CF488A Product List
Product Size Cat.No Price in €
CF488A-Labeled Secondary Antibody Conjugates
Donkey Anti-Goat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20016 174,00
(min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat)
Donkey Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20014 174,00
(min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human,
Rabbit, and Sheep)
Donkey Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20015 174,00
(min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human,
Mouse, Rat, and Sheep)
Donkey Anti-Rat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20027 174,00
(min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse,
Human, Rabbit, and Sheep)
Donkey Anti-Sheep IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20024 174,00
(min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat)
Goat Anti-Chicken IgY (IgG) (H+L) whole antibody, 2 mg/mL 0.5 mL 20020 174,00
(min X Bovine, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse,
Rabbit, Rat, and Sheep)
Goat Anti-Guinea Pig IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20017 141,00
Goat Anti-Human IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20022 174,00
(min X Bovine, Horse, and Mouse)
Goat Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20010 141,00
Goat Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL
(min x Human, Bovine, Horse, Rabbit, and Swine)
0.5 mL 20018 174,00
Goat Anti-Mouse IgG (H+L), F(ab’)2 fragment, 2 mg/mL 0.25 mL 20011 114,00
Goat Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20012 141,00
Goat Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20019 174,00
(min X Human, Mouse, and Rat)
Goat Anti-Rabbit IgG (H+L), F(ab’)2 fragment, 2 mg/mL 0.25 mL 20013 114,00
Goat Anti-Rat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20023 174,00
Goat Anti-Swine IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20028 141,00
Rabbit Anti-Goat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20021 141,00
Rabbit Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL(min X Human) 0.5 mL 20026 174,00
Rabbit Anti-Rat IgG (H+L) whole antibody, 2 mg/mL(min X Human) 0.5 mL 20025 174,00
Other CF488A-Labeled Products
Annexin V, 50 mg/mL 0.5 mL 29005 239,00
α-Bungarotoxin 0.5 mg 00005 408,00
Phalloidin 300 U 00042 316,00
Streptavidin 1 mg 29034 174,00
CF488A Reactive Dyes and Labeling Kits
CF488A, aminooxy 1 mg 92051 424,00
CF488A hydrazide 1 mg 92152 424,00
CF488A maleimide 1 µmole 92022 212,00
CF488A succinimidyl ester 1 µmole 92120 228,00
CF488A SE protein labeling kit 3 labelings (for 1 mg protein each) 92213 381,00
CF488A SE microscale protein labeling kit 3 labelings (for 20-100 µg protein each) 92233 120,00

CF TM 633 Dye
Truly the best dye for the 633/635 nm lasers
Technical Summary:
Abs/Em Maxima: 630/650 nm
Extinction coefficient: 100,000
Molecular weight: ~ 820
Flow cytometry laser line: 633 or 635 nm
Microscopy laser line: 633 or 635 nm
Direct replacement for: Alexa Fluor® 633, Alexa Fluor®
647, Cy5 and DyLight 649
Advantages
• Yields the brightest antibody conjugates among spectrally
similar dyes when excited by the 633 nm He-Ne laser
or the 635 nm red diode laser
• Far more photostable than Alexa Fluor® 647
• Highly water-soluble
Ordering information
Figure 1.
Absorption and emission
spectra of CF633
conju-gated to goat
anti-mouse IgG in PBS.
Figure 2.
Jurkat cells were stained
with intracellular CD3
or isotype control followed
by goat antimouse
IgG conjugates.
Fluorescence was analyzed
on a BD FACS Calibur
in the FL4 channel.
The bars represent the
relative fluorescence of
the geometric means of
the population of cells.
Far-red fluorescent dyes offer the advantage of ultra sensitive
detection because background signal due to autofluorescence
in most biological samples is minimal in this spectral region.
For many years, the cyanine dye Cy5 has been the dye of choice
for such detection. More recently, Alexa Fluor® 647 has been developed
as a better alternative by having brighter fluorescence
and higher photostability. Despite the improvement, Alexa
Fluor® 647 still lacks sufficient photostability required for many
demanding applications. On the other hand, while Alexa Fluor®
633 is photostable, its fluorescence on proteins is very weak.
In fact, it has been a challenging task for dye chemists to develop
a far-red fluorescent dye that is both highly fluorescent and
photostable for protein and nucleic acid labeling. Using new
chemistry, scientists at Biotium have successfully developed
CF633 to overcome these challenges. With its absorption peak
at 630 nm, CF633 is optimally excited by the 633 nm He-Ne
laser or the 635 nm red diode laser. Its emission maximum is at
650 nm, which is 15 nm shorter than that of Alexa Fluor® 647 or
Cy5. Although the detection window on most flow cytometers is
centered around the emission peak wavelength of Alexa Fluor®
647 or other Cy5-like cyanine dyes, CF633 is still significantly
brighter (Figure 2). The most important advantage of CF633,
however, is its unmatched photostability (Figure 3). The combination
of superior brightness and photostability make CF633
the best choice for any detection system using a 633 or 635 nm
laser excitation source.
A list of CF633-based products are shown in Table 1. A full
selection of secondary antibodies, antibody labeling kits, and
other bioconjugates including phalloidin, annexin V and α-bungarotoxin
are also available for many CF dyes. Please visit the
BIOTREND website at www.biotrend.com for details.
Figure 3.
Jurkat cells were fixed, permeabilized and stained with rabbit anti-CD3 (Abcam) followed
by CF633 (Biotium) or AlexaFluor® 647 (Invitrogen) goat anti-rabbit IgG conjugates.
Cells were imaged using an Olympus mercury arc lamp microscope equipped
with a Cy5 filter set and CCD camera. Images were taken at t=0, 1 min and 5 min.
Table 1. CF633 Product List
Product Size Cat.No Price in €
CF633-Labeled Secondary Antibody Conjugates
Donkey Anti-Goat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20127 174,00
(min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat)
Donkey Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20124 174,00
(min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse,
Rabbit, and Rat)
Donkey Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20125 174,00
(min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat,
and Sheep)
Donkey Anti-Rat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20137 174,00
(min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit,
and Sheep)
Donkey Anti-Sheep IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20134 174,00
(min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat)
Goat Anti-Chicken IgY (IgG) (H+L) whole antibody, 2 mg/mL 0.5 mL 20126 174,00
(min X Bovine, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit,
Rat, and Sheep)

... Table 1. CF633 Product List
Product Size Cat.No Price in €
CF633-Labeled Secondary Antibody Conjugates
Goat Anti-Guinea Pig IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20129 141,00
Goat Anti-Human IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20132 174,00
(min X Bovine, Horse, and Mouse)
Goat anti-mouse IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20120 141,00
Goat anti-mouse IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20121 174,00
(min x Human, Bovine, Horse, Rabbit, and Swine)
Goat anti-mouse IgG (H+L), F(ab’)2 fragment, 2 mg/mL 0.25 mL 20130 114,00
Goat Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20122 141,00
Goat Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20123 174,00
(min X Human, Mouse, and Rat)
Goat Anti-Rabbit IgG (H+L), F(ab’)2 fragment, 2 mg/mL 0.25 mL 20131 114,00
Goat Anti-Rat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20133 174,00
(min X Human, Bovine, Horse, and Rabbit)
Goat Anti-Swine IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20138 141,00
Rabbit Anti-Goat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20128 141,00
Rabbit Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL (min X Human) 0.5 mL 20136 174,00
Rabbit Anti-Rat IgG (H+L) whole antibody, 2 mg/mL (min X Human) 0.5 mL 20135 174,00
Other CF633-Labeled Products
Annexin V, 50 mg/mL 0.5 mL 29008 239,00
α-Bungarotoxin 0.5 mg 00009 326,00
Phalloidin 300 U 00046 316,00
Streptavidin 1 mg 29037 174,00
CF633 Reactive Dyes and Labeling Kits
CF633, aminooxy 1 mg 92053 422,00
CF633 hydrazide 1 mg 92156 163,00
CF633 maleimide 1 µmole 92026 212,00
CF633 succinimidyl ester 1 µmole 92133 228,00
CF633 SE protein labeling kit 3 labelings (for 1 mg protein each) 92217 381,00
CF633 SE microscale protein labeling kit 3 labelings (for 20-100 µg protein each) 92237 228,00
CF TM 640 Dye
A highly photostable far-red dye for the 633 and 640 nm lasers
Technical Summary:
Abs/Em Maxima: 642/662 nm
Extinction coefficient: 105,000
Molecular weight: 823
Flow cytometry laser line: 633, 635 or 640 nm
Microscopy laser line: 633, 635 or 640 nm
Direct replacement for: Cy5, Alexa Fluor® 647
and ATTO 647N
Advantages
• Has the best photostability among dyes
with Cy5-like spectra
• Yields highly fluorescent protein conjugates
• Very water-soluble and pH-insensitive
CF640R is also far brighter and more photostable than Atto
647N, another spectrally similar dye frequently used in singlemolecule
imaging. The combination of excellent brightness
and photostability makes CF640R ideal for confocal microscopy,
single-molecule imaging and other demanding applications
based on fluorescence detection.
A list of CF640R-based products are shown in Table 1. A full
selection of secondary antibodies, antibody labeling kits, and
other bioconjugates including phalloidin, annexin V and α-bungarotoxin
are also available for many CF dyes. For more information,
please visit the BIOTREND website at www.biotrend.com.
Figure 1.
Absorption and emission
spectra of CF TM 640R
and Cy TM 5 conjugated
to goat anti-mouse IgG
in PBS.
CF640R is a rhodamine-based far-red fluorescent dye with excitation
and emission maxima very similar to those of Cy5 and
Alexa Fluor® 647 (Figure 1). CF640R is much brighter than
Cy5 and at least as bright as Alexa Fluor® 647 (Figure 2).
A major advantage of CF640R over Cy 5 and Alexa Fluor® 647
is its exceptional photostability. Cy5 and Alexa Fluor® 647 are
cyanine-based dyes and, like other cyanine dyes in general, have
intrinsically poor photostability.

Ordering information
Table 1. CF640R Product List
Figure 2.
Jurkat cells were stained
with intracellular CD3
followed by goat antimouse
IgG conjugates.
Background staining
was determined by
secondary antibody
staining alone. Fluorescence
was analyzed
on a BD FACS Calibur
in the FL4 channel.
The bars represent the
relative fluorescence of
the geometric means of
the population of cells.
Figure 3.
Photostability comparison between CF640R and Alexa Fluor 647. HeLa cells were
stained with β-tubulin conjugates of CF640R or Alexa Fluor 647. Cells were continuously
illuminated by a mercury arc lamp microscope and sequential images were
captured. Shown above are representative images of stained cells at 0, 1 and 3 min
of photobleaching.
Product Size Cat.No Price in €
CF640R-Labeled Secondary Antibody Conjugates
Donkey Anti-Goat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20179 174,00
(min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat)
Donkey Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20177 174,00
(min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit,
and Sheep)
Donkey Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20178 174,00
(min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse,
Rat, and Sheep)
Donkey Anti-Rat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20199 174,00
(min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit,
and Sheep)
Donkey Anti-Sheep IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20083 174,00
(min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat)
Goat Anti-Chicken IgY (IgG) (H+L) whole antibody, 2 mg/mL 0.5 mL 20084 174,00
(min X Bovine, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit,
Rat, and Sheep)
Goat Anti-Guinea Pig IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20085 174,00
Goat Anti-Human IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20081 174,00
(min X Bovine, Horse, and Mouse)
Goat Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20175 174,00
(min x Human, Bovine, Horse, Rabbit, and Swine)
Goat Anti-Mouse IgG (H+L), F(ab’)2 fragment, 2 mg/mL 0.25 mL 20086 174,00
Goat Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20176 174,00
Goat Anti-Rabbit IgG (H+L), F(ab’)2 fragment, 2 mg/mL 0.25 mL 20087 143,00
Goat Anti-Rat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20088 174,00
Goat Anti-Swine IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20089 141,00
Rabbit Anti-Goat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20090 141,00
Rabbit Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL (min X Human) 0.5 mL 20200 218,00
Rabbit Anti-Rat IgG (H+L) whole antibody, 2 mg/mL (min X Human) 0.5 mL 20201 218,00
Other CF640R-Labeled Products
Annexin V, 50 mg/mL 0.5 mL 29014 239,00
α-Bungarotoxin 0.5 mg 00004 408,00
Phalloidin 300 U 00050 394,00
Streptavidin 1 mg 29041 174,00
Wheat germ agglutinin (WGA) 5x1 mg 29026 272,00
CF640R Reactive Dyes and Labeling Kits
CF640R, aminooxy 1 mg 92058 424,00
CF640R hydrazide 1 mg 92157 109,00
CF640R maleimide 1 µmole 92034 212,00
CF640R succinimidyl ester 1 µmole 92108 228,00
CF640R SE protein labeling kit 3 labelings (for 1 mg protein each) 92225 381,00
Mix-n-Stain CF640R antibody labeling kit, 1x(50-100 µg) labeling 1 labeling 92244 120,00
Mix-n-Stain CF640R antibody labeling kit, 1x(20-50 µg) labeling 1 labeling 92258 104,00
Mix-n-Stain CF640R antibody labeling kit, 1x(5-20 µg) labeling 1 labeling 92278 98,00
www.biotrend.com

CF TM 660C and CF TM 660R Dyes
Superior alternatives to Alexa Fluor® 660
Technical Summary:
Abs/Em Maxima: 667/685 nm
Extinction coefficient: 200,000
Molecular weight: 3112
Flow cytometry laser line: 633 or 635 nm
Microscopy laser line: 633 or 635 nm
Direct replacement for: Alexa Fluor® 660,
Allophycocyanin (APC)
Advantages
• Much brighter than Alexa Fluor® 660
• More photostable than Alexa Fluor® 660
• Highly water-soluble
Technical Summary:
Abs/Em Maxima: 663/682nm
Extinction coefficient: 100,000
Molecular weight: 888
Flow cytometry laser line: 633 or 635 nm
Microscopy laser line: 633 or 635 nm
Direct replacement for: Alexa Fluor® 660,
Allophycocyanin (APC)
CF TM 660C
CF TM 660R
Advantages
• Brighter than Alexa Fluor® 660
• The most photostable 660 nm dye ideal for confocal
microscopy application
• Highly water-soluble
Figure 1.
Absorption and emission
spectra of CF660C and
CF660R conjugated
to goat anti-mouse IgG,
respectively, in PBS.
Figure 2.
Relative fluorescence of
CF660C-, CF660R and
Alexa Fluor 660-goat
anti-mouse conjugates
as a function of number
of dye per protein
(i.e., degree of labeling).
Fluorescence was measured
at each dye’s
emission maximum in
PBS using 633 nm
excitation.
CF660C and CF660R are two spectrally similar fluorescent
dyes that emit fluorescence at about 685 nm in the borderline
spectral region between far-red and near-IR (Figure 1). Although
their absorption maxima are at around 660 nm, both dyes can
be sufficiently excited by the 633 or 635 nm laser. When combined
with other CF dyes of shorter wavelengths, CF660C or
CF660R can serve as a useful long wavelength dye in multicolor
detection applications. The two dyes are spectrally similar
to Alexa Fluor® 660 but are far superior to the latter in performance.
Like Alexa Fluor® 660, CF660C is a cyanine-based dye.
However, when conjugated to protein, CF660C is several fold
brighter and significantly more photostable than Alexa Fluor® 660
(Figures 2 and 3). CF660R is a rhodamine-based dye. Like rhodamine
dyes in general, CF660R is exceptionally photostable,
compared to both Alexa Fluor® 660 and CF660C (Figure 2).
CF660R is also much brighter than Alexa Fluor® 660, though
not as bright as CF660C (Figure 2). The superior photostability
and excellent brightness of CF660R make the dye an ideal choice
for confocal microscopy and other demanding applications.
A list of CF660C- and CF660R-based products are shown in
Table 1. A full selection of secondary antibodies, antibody labeling
kits, and other bioconjugates including phalloidin, annexin V
and α-bungarotoxin are also available for many CF dyes. Please
visit the BIOTREND website at www.biotrend.com for details.
Figure 3.
HeLa cells were fixed,
permeabilized and
stained with mouse
α-tubulin followed by
CF660C or AlexaFluor®
660 goat anti-mouse
IgG conjugates. Cells
were imaged using an
Olympus mercury arc
lamp microscope
equipped with a Cy5
filter set and CCD camera.
Images were taken
at t=0 and 10 seconds.
Figure 4.
HeLa cells were fixed,
permeabilized and
stained with mouse
α-tubulin followed by
CF660C, CFF660R or
AlexaFluor® 660 goat
anti-mouse IgG conjugates.
Cells were imaged
using an Olympus
mercury arc lamp microscope
equipped with
a Cy5 filter set and CCD
camera. The graph illustrates
the relative fluorescence
intensities
of sequential images
taken every 10 seconds
for 5 minutes.

Ordering information
(...CF TM 660C and CF TM 660R Dyes)
Table 1. CF660C and CF660R Product List
Product Size Cat.No Price in €
CF660C- and CF660R-Labeled Secondary Antibody Conjugates
CF660C Donkey Anti-Goat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20051 174,00
(min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat)
CF660C Goat Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20050 141,00
CF660C Goat Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20052 174,00
(min x Human, Bovine, Horse, Rabbit, and Swine)
CF660C Goat Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20053 141,00
CF660R Goat Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20054 141,00
CF660R Goat Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20055 141,00
Other CF660R-Labeled Products
CF660R Phalloidin 300 U 00047 316,00
CF660R Streptavidin 1 mg 29040 174,00
CF660C and CF660R Reactive Dyes and Labeling Kits
CF660C succinimidyl ester 1 µmole 92137 228,00
CF660C SE protein labeling kit 3 labelings (for 1 mg protein each) 92219 381,00
CF660C SE microscale protein labeling kit 3 labelings (for 20-100 µg protein each) 92239 120,00
CF660R succinimidyl ester 1 µmole 92134 228,00
CF660R SE protein labeling kit 3 labelings (for 1 mg protein each) 92223 381,00
CF660R SE microscale protein labeling kit 3 labelings (for 20-100 µg protein each) 92243 120,00
CF TM 680C and CF TM 680R Dyes
Superior alternatives to Alexa Fluor® 660
Technical Summary:
Abs/Em Maxima: 681/698 nm
Extinction coefficient: 210,000
Molecular weight: 3241
Direct replacement for: Alexa Fluor® 680, Cy5.5
Advantages
• The brightest among spectrally similar 680 nm dyes
• Superior signal-to-noise ratio in immunostaining
• Highly water-soluble and pH-insensitive
Technical Summary:
Abs/Em Maxima: 608/701 nm
Extinction coefficient: 140,000
Molecular weight: 912
Direct replacement for: Alexa Fluor® 680, Cy5.5
CF TM 680C
CF TM 680R
Advantages
• The most photostable 680 nm dye
• suitable for labeling nucleic acids and small bio-molecules
• Highly water-soluble and pH-insensitive
CF680 and CF680R are two outstanding near-IR dyes excitable
at about 680 nm with emission at about 700 nm.
The two dyes each have unique properties suitable for different
application needs.
CF680 is a highly water-soluble cyanine-based dye with a molecular
weight of ~ 3000. This dye is excellent for labeling antibodies,
producing the brightest fluorescence and highest signalto-noise
ratio in immunostaining among spectrally similar dyes,
such as Cy5.5, Alexa Fluor 680, DyLight 680 and IRDye 680.
However, because of its relatively large molecular size, CF680 is
not recommended for labeling nucleic acids or relatively small
biomolecules, for which our CF680R is better suited.
CF680R is a novel rhodamine-based dye with a relatively small
molecular weight of 912. The dye is highly fluorescent and, more
importantly, extremely photostable. Rhodamine dyes are traditionally
known to be bright and photostable. However, it has
been a synthetic challenge to make functional rhodamine dyes
with long wavelengths and high water solubility necessary for
bio-labeling. Scientists at Biotium have overcome the challenge
and invented a new way to make highly water soluble, bright and
photostable rhodamine dyes with wavelengths ranging from red
to near-IR. These dyes are excellent for labeling proteins, nucleic
acids and small bio-molecules. They are ideal for confocal microscopy,
single molecule-based imaging and other applications
that demand both brightness and photostability.
A list of CF680- and CF680R-based products are shown in
Table 1. A full selection of secondary antibodies, antibody labeling
kits, and other bioconjugates including phalloidin, annexin V
and α-bungarotoxin are also available for many CF dyes. Please
visit the BIOTREND website at www.biottrend.com for details.
Figure 1.
Absorption and emission
spectra of CF680 and
CF680R conjugated
to goat anti-mouse IgG,
respectively, in PBS.

Applications
Figure 2.
Jurkat cells were stained
with isotype or mouse
anti-human intracellular
CD3 antibody followed by
1 mg of goat anti-mouse
IgG conjugated with
Cy5.5, AlexaFluor®680,
CF680 or DyLight680.
Fluorescence was detected
by a BD FACS Calibur
in the FL4 channel.
The bars represent the
signal-to-noise ratio of
CD3 positive fluorescence
to isotype using similar
degrees of labeling (DOL).
In Vivo Imaging
CF reactive dyes and kits for small animal imaging
Westerns
Perform Westerns with highly cross-adsorbed antibodies
to minimize cross-reactivity and background
Protein Labeling
Reactive dyes and kits for custom conjugations
Figure 4.
HeLa cells were fixed,
permeabilized and
stained with mouse
α-tubulin followed by
CF660C, CFF660R or
AlexaFluor® 660 goat
anti-mouse IgG conjugates.
Cells were imaged
using an Olympus
mercury arc lamp microscope
equipped with
a Cy5 filter set and CCD
camera. The graph illustrates
the relative fluorescence
intensities
of sequential images
taken every 10 seconds
for 5 minutes.
Figure 5.
HeLa cells were stained
with mouse a-tubulin
antibody followed by
CF680 goat antimouse
IgG. Images were
captured using an
Olympus mercury arc
lamp microscope
equipped with a Cy5
filter set, CCD camera
and ImagePro Express
software.
Flow Cytometry
Superior non-tandem conjugates for cell staining
Microscopy
Bright and photostable antibodies or reactive dyes
formultiple labeling procedures
(Please also see our other superior near-IR CF dyes, including
CF750, CF770 an CF790)
Figure 3.
Photostability comparison
by microscopy.
Jurkat cells were fixed,
permeabilized and
stained with mouse
anti-CD3 followed by
CF680R (Biotium),
AlexaFluor®680
(Invitrogen) or Cy5.5
(GE Healthcare) goat
anti-mouse IgG conjugates.
Cells were imaged
using an Olympus mercury
arc lamp microscope
equipped with
a Cy5 filter set and CCD
camera. Images were
taken at t=0, 1 min and
5 min.
Figure 6.
Near-IR CF680 and CF770 for two-color Western blotting.
Two-fold dilutions of HeLa cell lysate were run on an acrylamde gel, transferred
to a nitrocellulose membrane and probed with mouse alpha-tubulin and rabbit COX IV
primary antibodies followed by goat antimouse CF770 or IRDye 800 (green)
and goat anti-rabbit CF680 or IRDye680 (red) at the same final concentrations.
After probing, membranes were dried and scanned using an Odyssey® infrared
ima-ging system (Li-Cor Biosciences). Quantitation of the bands showed
approximately a 3.5-fold higher fluorescence intensity of CF dyes compared
to the respective IRDye secondary antibodies (Li-Cor)
All products listed in this brochure are for research use only.
Not for use in diagnostic or therapeutic procedures.
CF is a trademark of Biotium; CF dye technologies are covered
by pending US and international patents.
Alexa Fluor is registered trademark of Invitrogen.
Cy is trademark of GE Healthcare.
IRDye and Odyssey are trademarks of Li-Cor Biosciences.

Ordering information
(...CF TM 680C and CF TM 680R Dyes)
Table 1. CF680C and CF680R Product List
Product Size Cat.No Price in €
CF680C- and CF680R-Labeled Secondary Antibody Conjugates
CF680 Donkey Anti-Goat IgG (H+L) whole antibody, 2 mg/mL 0.25 mL 20060 174,00
(min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat)
CF680R Donkey Anti-Goat IgG (H+L) whole antibody, 2 mg/mL 0.25 mL 20196 185,00
CF680R Donkey Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL 0.25 mL 20194 185,00
(min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human,
Rabbit, and Sheep)
CF680R Donkey Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.25 mL 20195 185,00
(min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse,
Rat, and Sheep)
CF680 Donkey Anti-Sheep IgG (H+L) whole antibody, 2 mg/mL 0.25 mL 20062 174,00
(min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat)
CF680 Goat Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL 0.25 mL 20065 174,00
(min x Human, Bovine, Horse, Rabbit, and Swine)
CF680 Goat Anti-Mouse IgG (H+L), F(ab’)2 fragment, 2 mg/mL 0.25 mL 20063 141,00
(min x Human, Bovine, Horse, Rabbit, and Swine)
CF680R Goat Anti-Mouse IgG (H+L) whole antibody, 2 mg/mL 0.25 mL 20192 185,00
(min x Human, Bovine, Horse, Rabbit, and Swine)
CF680 Goat Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.25 mL 20067 174,00
(min X Human, Mouse, and Rat)
CF680 Goat Anti-Rabbit IgG (H+L), F(ab’)2 fragment, 2 mg/mL 0.25 mL 20064 141,00
CF680R Goat Anti-Rabbit IgG (H+L) whole antibody, 2 mg/mL 0.25 mL 20193 185,00
(min X Human, Mouse, and Rat)
CF680 Goat Anti-Rat IgG (H+L) whole antibody, 2 mg/mL 0.25 mL 20069 174,00
(min X Human, Bovine, Horse, and Rabbit)
CF680 Rabbit Anti-Goat IgG (H+L) whole antibody, 2 mg/mL 0.5 mL 20068 163,00
Other F680- and CF680R-Labeled Products
CF680R Phalloidin 300 U 00048 316,00
CF680 Wheat Germ Agglutinin (WGA) 5 mg 29025 294,00
CF680C and CF680R Reactive Dyes and Labeling Kits
CF680 succinimidyl ester 1 µmole 92139 245,00
CF680 SE protein labeling kit 3 labelings (for 1 mg protein each) 92220 381,00
CF680 SE microscale protein labeling kit 3 labelings (for 20-100 µg protein each) 92240 120,00
CF680 maleimide 1 µmole 92029 212,00
CF680R succinimidyl ester 1 µmole 92107 272,00
CF680R SE protein labeling kit 3 labelings (for 1 mg protein each) 92226 381,00
CF680R maleimide 1 µmole 92032 250,00
www.biotrend.com

Near-IR CF TM Dyes
Simply the brightest and most stable long wavelength dyes
Long Wavelength CF Dyes Ideal for In Vivo Imaging
and Other Near-IR Detection
Developed by scientists at Biotium, Near-IR CF dyes are a group
of fluorescent dyes with absorption and emission wavelengths
between 650 and 800 nm. Designed for protein labeling, near-IR
CF dyes are significantly brighter and more stable than any other
commercial dye of similar wavelengths.
Near-IR dyes offer important advantages
over traditional visible light dyes. Because
cellular or tissue components produce minimal
autofluorescence in the near-IR
region, near-IR dyes have the potential to
offer highly specific and sensitive detection
in complex biological systems. Also,
because light with wavelength in the near-
IR region has strong tissue penetration,
near-IR dyes are ideal for in vivo fluorescence
imaging, an emerging field that has
advanced rapidly in recent years. Futhermore,
near-IR dyes are also excellent dyes
for in- or on-cell and membrane-based
Western assays.
Near-IR CF dyes are next-generation long wavelength dyes representing
a true breakthrough in the field. The current commercial
near-IR dyes suffer from problems of limited fluorescence brightness
due to excessive dye aggregation and poor stability. As a
result of novel molecular engineering by scientists at Biotium,
near-IR CF dyes overcome these problems, resulting in several
key advantages over other competitive dyes. Near-IR CF dyes are
exceptionally bright and stable. For example, our CF750 is so
bright, it can be excited at 633 nm (i.e., at the shoulder wavelength
of the absorption maximum) but still emits stronger fluorescence
at ~ 770 nm than APC-based tandem dyes, making the
dye particularly useful for flow cytometry applications without the
spillover and stability challenges encountered with tandem dyes.
Figure 1.
Stability of Cy7,
AlexaFluor750
and CF750 dyes.
Shown are the absorption
spectra of the
respective dyes before
(black line) and after
(gray line) 30 minutes
of exposure to sunlight.
* Compatible with the
LI-COR Odyssey system
** Ex/Em wavelengths
based on antibody
conjugates
Products
• Reactive Dyes
• Secondary Antibodies
• Labeling Kits
• Other Labeled Reagents
Advantages
• Brightness
CF dyes match or surpass the brightness
of Alexa✟ Fluor, Cy✟ and other commercially
available dyes.
• Photostability
CF dyes are among the most photostable dyes.
• Specificity and in-vivo Stability
CF-labeled conjugates offer superior specificity
and improved half-life for in-vivo imaging.
• pH Insensitivity
CF dyes remain highly fluorescent over a wide pH range.
• Solubility
CF dyes are highly soluble in both water and a wide range
of organic solvents.
• Compatibility
CF dyes are spectrally similar to other commercially
available dyes and compatible with common excitation
sources and existing filters.
• Color Selection
CF dye series offers the largest dye collection
with wavelengths ranging from UV to near IR.
Figure 2.
Jurkat cells were stained
with isotype or mouse
anti-human CD3 antibody
followed by goat
anti-mouse APC-Alexa-
Fluor750 (Invitrogen)
or CF750 using the
amount of antibody
shown. Flourescence was
analyzed using a BD LSR
II flow cytometer
equipped with a 633 nm
laser and 780/60 nm
PMT detector. Bars represent
the relative
fluorescence values of
the geometric means.
*CF dye technology is covered by pending US and international patents;
Alexa is a registered trademark of Invitrogen.; Cy is a trademark
of GE Healthcare; DyLight is a trademark of Thermo Fisher Scientific;
and IRDye and Odyssey are registered trademarks of Li-COR Biosciences;
in-cell Western is a trademark of Li-Cor Biosciences..
Figure 3.
Bars represent the signal-to-noise
ratio of
the relative fluorescence
values of the geometric
means from Figure 2.

...Near-IR CF TM Dyes
Simply the brightest and most stable long wavelength dyes
In addition, near-IR CF dyes possess a proprietary structural feature
that renders the dyes less immunogenic than other competitive
dyes. Consequently, a higher number of near-IR CF dyes can
be conjugated to a protein for maximal fluorescence, and the
resulting protein conjugate be expected to have a longer in vivo
half-life. Finally, near-IR CF dyes have much higher labeling efficiency
than other near-IR dyes because of their excellent solubility
and generally >95% reactivity.
Figure 4.
Goat anti-mouse IgG
was labeled with CF750,
AlexaFluor750 or Cy7,
respectively. Fluorescence
values were
measured on a Hitachi
F-4500 fluorometer.
The normalized fluorescence
values are shown
above.
Figure 5.
Goat anti-mouse IgG
was labeled with CF750,
AlexaFluor750 or Cy7.
Absorbance of the conjugated
dyes were normalized
to their respective
absorbance max.
Cy7 and AlexaFluor750
have large shoulder
peaks which are indicative
of dye aggregation
and do not contribute
to overall fluorescence
intensity.
Figure 6.
Jurkat cells were stained
with isotype or mouse
anti-human intracellular
CD3 antibody followed
by 1 ug of goat antimouse
IgG conjugated
with Cy5.5,
AlexaFluor 680, CF680
or DyLight680. Fluorescence
was detected by
a BD FACS Calibur in the
FL4 channel. The bars
represent the signalto-noise
ratio of CD3
positive fluorescence
to isotype using similar
degrees of labeling (DOL).
Figure 7.
HeLa cells were stained
with mouse alpha-tubulin
antibody followed by
CF680 goat anti-mouse
IgG. Images were captured
using an Olympus
mercury arc lamp microscope
equipped with
a Cy5 filter set, CCD camera
and ImagePro Express
software.
Figure 8.
Jurkat cells were stained
with isotype or intracellular
CD3 antibody followed
by 1 ug of goat
anti-mouse IgG conjugated
with CF770,
DyLight 800 or IRDye
800CW, respectively.
Fluorescence was measured
on a BD LSR II
equipped with a 633 nm
laser and 780/60 nm
PMT detector. The bars
represent the relative
flurorescence values of
the geometric means.
Figure 9.
Two-fold dilutions of
HeLa cells were cultured
in a black, clear bottom
96-well plate. Cells were
fixed, permeabilized
and probed with mouse
alpha-tubulin and rabbit
COX IV primary antibodies
followed by goat
anti-mouse CF770 or
IRDye 800 (green) and
goat anti-rabbit CF680
or IRDye680 (red) at
the same final concentrations.
After probing,
cells were dried and
scanned using an
Odyssey® infrared imaging
system (Li-Cor).
The upper two rows were
probed with CF dye conjugates
while the lower
two rows were probed
with commercially
purchased IRDye secondaries
(Li-Cor).

Figure 10.
Two-fold dilutions of
HeLa cell lysate were run
on an acrylamde gel,
transferred to a nitrocellulose
membrane and
probed with mouse
alpha-tubulin and rabbit
COX IV primary antibodies
followed by goat
anti-mouse CF770 or
IRDye 800 (green) and
goat anti-rabbit CF680
or IRDye680 (red) at
the same final concentrations.
After probing,
membranes were dried
and scanned using an
Odyssey® infrared imaging
system (Li-Cor Biosciences).
Quantitation
of the bands showed
approximately a 3.5-fold
higher fluorescence
intensity of CF dyes compared
to the respective
IRDye secondary antibodies
(Li-Cor).
Images courtesy of Caliper Life Sciences.
Figure 11.
Tumors in mice were imaged
24 and 96 hrs after
i.v. injection of Avastin
conjugated CF750 using
an IVIS imaging system
(Caliper LS).
List of CF TM Dye Product Categories*
Table 1.
Product Type Application Unit Size Price in €
CF dye NHS esters Labeling antibodies and other biomolecules 1 µmole Enquire
VivoBrite near-IR CF dye labeling kit for small animal in vivo imaging Labeling antibodies for in vivo imaging 3 labelings Enquire
CF dye goat anti-mouse IgG (H+L), 2 mg/mL Microscopy, flow cytometry and Western blotting 0.5 mL Enquire
CF dye goat anti-rabbit IgG (H+L), 2 mg/mL Microscopy, flow cytometry and Western blotting 0.5 mL Enquire
CF dye goat anti-guinea pig IgG (H+L), 2 mg/mL Microscopy, flow cytometry and Western blotting 0.5 mL Enquire
CF dye F(ab')2 fragment of goat anti-mouse IgG (H+L), 2 mg/mL Microscopy, flow cytometry and Western blotting 0.25 mL Enquire
CF dye F(ab')2 fragment of goat anti-rabbit IgG (H+L), 2 mg/mL Microscopy, flow cytometry and Western blotting 0.25 mL Enquire
Near-IR CF dye goat anti-mouse IgG (H+L), 2 mg/mL Microscopy, flow cytometry and Western blotting 0.5 mL Enquire
Near-IR CF dye goat anti-rabbit IgG (H+L), 2 mg/mL Microscopy, flow cytometry and Western blotting 0.5 mL Enquire
Near-IR CF dye goat-anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL Microscopy, flow cytometry and Western blotting 0.25 mL Enquire
Near-IR CF dye goat-anti-rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL Microscopy, flow cytometry and Western blotting 0.25 mL Enquire
CF dye annexin V conjugates, 50 ug/mL Apoptosis 0.5 mL Enquire
CF dye phalloidin conjugates F-actin staining 300 U Enquire
*For a complete list and descriptions of individual products, please visit BIOTREND website: www.biotrend.com
www.biotrend.com

CF TM Dye Selection Table
Page 1
CF TM dye λEx (nm) λEm (nm) MW ε Excitation source* Direct replacement for: Advantages
CF 350 347 448 496 18000 UV AMCA, Cascade Blue®,
Coumarin,
DyLight 350
CF405S 404 431 1169 33000 405 nm laser Alexa Fluor® 405,
DyLight 405
CF405M 408 452 503 41000 405 nm laser BD Horizon V450,
eFluor® 450, Pacific Blue
• Yields the brightest blue fluorescent antibody
conjugates when excited at ~ 350 nm
• Highly water-soluble and insensitive to pH
• Much brighter than Alexa Fluor® 405 due to
better compatibility with excitation and
emission windows on common instruments
• Highly water-soluble and insensitive to pH
• More photostable than Pacific Blue® dye
• As bright as Pacific Blue® dye in the blue channel
• Less spill-over fluorescence in the green channel
• Highly water-soluble
CF485 487 513 602 70000 470 nm LED Pacific Orange
488 nm laser
CF488A 490 515 914 70000 488 nm laser ATTO 488,
Alexa Fluor® 488, Cy2,
DY-495, DyLight488,
FAM, FITC, Fluorescein
CF555 555 565 810 150000 532 nm Alexa Fluor® 555,
or 568 nm laser ATTO 550, Cy3, DY-547,
DyLight549, TRITC
CF568 562 583 714 100000 532 nm Alexa Fluor® 568,
or 568 nm laser ATTO 565, DY-560,
Rhodamine Red
CF594 593 614 729 115000 532 nm Alexa Fluor® 594,
or 594 nm laser ATTO 594, DY-594,
DyLight 594, Texas Red®
CF620R 617 639 738 115000 633 nm LightCycler® Red 640
or 635 nm laser
CF633 630 650 821 100000 633 nm Alexa Fluor® 633,
or 635 nm laser DY-630, DyLight 633
CF640R 642 662 832 105000 633 nm, 635 nm, ATTO 647N, Cy5
or 640 nm laser
• Unique green fluorescent dye with absorption
and emission wavelengths significantly shorter
than those of other common green dyes
• Excitable by 470 nm blue LED light source
• Minimal fluorescence in the red detection channel
in flow cytometry or PCR**
• Minimal charge compared to Alexa Fluor® 488
reduces nonspecific antibody staining
• Less spill-over fluorescence in the red channel
than Alexa Fluor® 488
• Extremely photostable
• Highly water-soluble and pH-insensitive
• Brighter and more photostable than Cy®3
• Minimal charge compared to Alexa Fluor®
555 reduces nonspecific antibody staining
• Optimal for the 568 nm line of the Ar-Kr
mixed-gas laser
• Brighter and more photostable than
Alexa Fluor® 568
• Yields the brightest antibody conjugates among
spectrally similar dyes
• Extremely photostable
• Excellent energy acceptor for FRET
• Highly fluorescent
• Extremely photostable
• Highly water-soluble
• Yields the brightest antibody conjugates among
spectrally similar dyes Optimal for 633 nm He-Ne
laser or the 635 nm red diode laser
• Far more photostable than Alexa Fluor® 647
• Highly water-soluble
• Most photostable among spectrally similar dyes
• Yields highly fluorescent protein conjugates
• Very water-soluble and pH-insensitive

CF TM Dye Selection Table
Page 2
CF TM dye λEx (nm) λEm (nm) MW ε Excitation source* Direct replacement for: Advantages
CF647 650 665 836 240000 633 nm, 635 nm, Alexa Fluor® 647,
or 640 nm laser Cy5, DY- 647,
DyLight 649
CF660C 667 685 3112 200000 633 nm, 635 nm, Alexa Fluor® 660
or 640 nm laser
CF660R 663 682 888 100000 633 nm, 635 nm, Alexa Fluor® 660
or 640 nm laser
CF680 681 698 3241 210000 680 nm Alexa Fluor® 680, Cy5.5,
or 685 nm laser DyLight 680, IRDye® 680
CF680R 680 701 912 140000 680 nm Alexa Fluor® 680,
or 685 nm laser Cy5.5, DyLight 680,
IRDye® 680
• Antibody conjugates have the best signal-to-noise
ratio compared to Alexa Fluor® 647, DyLight 649
and Cy 5
• Highly fluorescent
• Highly water-soluble and pH-insensitive
• Much brighter than Alexa Fluor® 660
• More photostable than Alexa Fluor® 660
• Highly water-soluble
• Brighter than Alexa Fluor® 660
• The most photostable 660 nm dye, ideal for
confocal microscopy
• Highly water-soluble
• The brightest among spectrally similar dyes
• Superior signal-to-noise ratio in immunostaining
• Highly water-soluble and pH-insensitive
• The most photostable 680 nm dye
• Suitable for labeling nucleic acids and small
bio-molecules
• Highly water-soluble and pH-insensitive
• CF680/CF770 double labeling compatible
with Li-COR Odyssey System
CF750 755 777 3009 250000 680 nm, 685 nm, Alexa Fluor® 750, Cy7,
or 785 laser DyLight 750,
APC- Alexa Fluor® 750
CF770 770 797 3138 220000 785 nm laser DyLight 800
IRDye® 800CW
CF790 784 806 3267 210000 785 nm laser Alexa Fluor® 790
• Exceptionally bright and stable
• Less immunogenic than competing dyes
• Better signal-to-noise ratio compared
to APC- Alexa Fluor® 750 tandem dye with
633 nm excitation
• Exceptionally bright and stable
• Less immunogenic than competing dyes
• CF680/CF770 double labeling compatible
with Li-COR Odyssey System
• Exceptionally bright and stable
• Less immunogenic than competing dyes
* Most fluorescent dyes can be excited using a UV light source for epifluorescence microscopy;
fluorescence wavelengths longer than ~ 650 nm are not visible to the human eye.
** Not recommended for fluorescence microscopy applications
CF dye technology is covered by pending US and international patents.
Alexa Fluor®, Cascade Blue®, Pacific Blue, Pacific Orange, and Texas Red® are trademarks of Invitrogen; ATTO dyes are products of ATTO-TEC GmbH; BD Horizon is a trademark
of BD Biosciences; Cy is a trademark of GE Healthcare; DY-dyes are products of Dyomics GmbH; DyLight® is a registered trademark of Thermo Fisher Scientific; eFluor® is a registered trademark
of eBioscience; IRDye® is a registered trademark of LI-COR Bioscience; LightCycler® is a registered trademark of Roche Applied Science

cts for Life Sciences Research and Bioanalysis • Products for Life Sciences Research and Bio
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