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2 7 , 8
Vol1 27 ,No1 8 ,pp159621599
2 0 0 7 8 Spectroscopy and Spectral Analysis August , 2007
1 , 2 , 1 , 2
1. , 421001
2. , 410082
, ,
,
, , ;
, ,
,
, KD 2821 3 mg mL - 1 , 319 ,
, C C , ,
; ; ;
: O65713 : A : 100020593 (2007) 0821596204
,
[125 ]
( Sulfamethazine , SM2 )
, ,
, ,
, , SM2
,
SM2 (MRL s) 50100
g kg - 1 , ,
, ,
,
,
[628 ]
1
211
(SM2 , , Sigma ) ,
(Sulfisoxazole SIZ , , Sigma ) ,
( EGDMA , , , )
(MAA , , ) ,
(, ) , (,
) , () , ()
UV28500 2(
) , 5502 ( ) ,
SHA2C () , SB3200
() , 366 nm (
) ,
112
01 278 3 g (1 mmol) 15 mL
, 01344 4 g (4 mmol) ,
31 964 4 g (20 mmol) , 12 h ,
120 mg ,
, 15 min , 5 min ,
: 2006205210 , : 2006208220
: (2000 K52141) , (06FJ3088) (B2004275)
: , , 1967 , e2mail : liuhuijun @vip1 sina1com

8
1597
, 366 nm 0
48 h , ,
10 %
, 60 24 h , PS24
P0 ,
PS24
113
25 mg ,
10 mL , 5 mL 015 mmol L - 1
SM2 SIZ , 25 24
h , 6 000 r min - 1 10 min , 1 mL ,
10 mL 2,
SM2 SIZ
SM2 SIZ , ( SM2
SIZ) Q ,
114
,
10 mL , 1 mmol L - 1
11 0 mL , ,
, 200350 nm
[9 , 10 ]
PS24 KBr
, 4 000400 cm - 1 ,
[11 ]
2
211
, SM2 SM2
SIZ ,
KD , , 1
2
Table 1 Adsorption amounts of two substrates on PS24 and P0
Q/ (mmol g - 1 ) Q/ (mmol g - 1 )
PS24 SM2 MAA SM2 1 01414 SIZ 27315
P0 MAA SM2 14414 SIZ 14815
Table 2 KD( mg mL - 1 ) andof different substrates on polymers
KD (mg mL - 1 ) KD (mg mL - 1 )
PS24 SM2 MAA SM2 28213 11 00 SIZ 7311 319
P0 MAA SM2 4012 11 00 SIZ 3917 11 01
KD [12 ]
KD
= Cp / Cs
Cp (mg g - 1 ) ,
, Cs (mg mL - 1 )
= KD i
/ KD j
ij , i = j , = 110
1 , PS24 SM2 P0 ,
SM2 SIZ 2
, SIZ , PS24 SM2
, P0
SM2
Fig11 UV adsorption spectra of SM2 and
, ,
MAA system in acetonitrile solution
The concentration of SM2 is 1 mmol L - 1 , and t he concentration of
212
MAA is : 1 : 0 ; 2 : 1 ; 3 : 2 ; 4 : 3 ; 5 : 4 ; 6 : 5 mmol L - 1
1 , ,
, , ,
[13 ,14 ] 3
, [15 ]
1
,

1598
27
, MAA H + SM2 N
N H + , COO - , N H + COO -
, SM2 , ,
(
[16 ] ) , ,
,
,
2
Fig12 Schematic representation of the preparation of sulfamethazine template polymer
213
( EGDMA )
(MAA) 3 4 , ,
3 1 723 cm - 1 EGDMA C O
, 1 638 cm - 1 C
C 4
3 1092 880 cm - 1 O H
, 1 635 cm - 1 C
C PS24
5 , 5 , 1 638 cm - 1 C
,
, 2 998 cm - 1
O H 1 738
cm - 1 C
O , SM2
MAA ,
C
Fig15 The IR spectra of solutions of
molecularly imprinted polymer( PS24)
, , ,
,
,
3
Fig1 3 The IR spectra of solutions of EGDMA
Fig14 The IR spectra of solutions of MAA
, ,
,
, SM2
MAA ,
; SM2
SM2 , KD
28213 mg mL - 1 , 319
:
,
, ,
,

8
1599
[ 1 ] Haginaka J , Takehira H , Hosoya K , et al. J . Chromatogr. A , 1999 , 849 (2) : 331.
[ 2 ] Haupt K , Noworyta K , Kutner K. Anal. Commun. , 1999 , 36 (11212) : 391.
[ 3 ] Mosbach K. Anal. Chim. Acta , 2001 , 435 : 3.
[ 4 ] Mullett W M , Dirie M F , Lai E P C , et al. Anal. Chim. Acta , 2000 , 414 , 123.
[ 5 ] Crescenzi C , Bayoudh S , Conmack , et al. Anal. Chem. , 2000 , 73 (10) : 2171.
[ 6 ] L IU Hui2jun , XIAN G Wei2zhong , XU Wei2jian , et al (, , , ) . Chin J . App . Chem. () , 2005 , 22 (1) :
83.
[ 7 ] ZHOU Jie , HE Xi2wen , YAN G Wen2long , et al ( , , , ) . Chem. J . Chinese Universities () ,
1999 , 19 (9) : 1388.
[ 8 ] MEN G Zi2hui , WAN G Jin2fang , ZHOU Liang2mo , et al (, , , ) . Chin. J . Chromatography () , 1999 , 17
(4) : 323.
[ 9 ] GAO Feng , ZHAN G Li , BIAN Gui2rong , et al ( , , , ) . Spectroscopy and Spectral Analysis (
) , 2005 , 25 (11) : 1843.
[10 ] WEI Yong2ju , L IU Cui2ge , MO Li2ping (, , ) . Spectroscopy and Spectral Analysis() , 2005 , 25
(1) : 86.
[11 ] L IU Shu2fen , J I Chun2nuan , ZHEN G Ke , et al (, , , ) . Spectroscopy and Spectral Analysis() ,
2003 , 23 (4) : 695.
[12 ] Jie Z , Xiwen H S. Anal. Chim. Act . , 1999 , 381 : 85.
[13 ] YAN Shou2lei , GAO Zhi2xian , FAN G Yan2jun (, , ) . Acta Polymerica Sinica () , 2006 , (1) : 160.
[ 14 ] DON G He , TON G Ai2jun , L I Long2di ( , , ) . Chem. J . Chinese Universities () , 2002 , 23 (6) :
1018.
[ 15 ] L I Xia , REN Gui2fen , L IU Meng2you ( , , , ) . Spectroscopy and Spectral Analysis () , 2004 ,
24 (11) : 1410.
[ 16 ] Mat sui J un , et al. Anal. Chem. , 1995 , 67 : 4404.
Preparation and Spectral Characteristic of Molecularly Imprinted
Polymers for Sulfamethazine
L IU Hui2jun 1 , XU Wei2jian 2 , L IU Yao2chi 1 , XION G Yuan2qin 2
1. College of Chemistry and Chemical Engineering , University of South China , Hengyang 421001 , China
2. College of Chemistry and Chemical Engineering , Hunan University , Changsha 410082 , China
Abstract Molecularly imprinted polymers (MIPs) were synthesized , which was initiated by UV photo2induction at low tempera2
ture , with sulfamethazine (SM2 ) was used as template , methacrylic acid (MAA) as monomer , and ethylene glycol dimethylacry2
late ( EGDMA) as crosslinker. By UV spectrometry the authors compared the combination action on sulfamethazine with MAA
and researched the static adsorption ability and selective recognition of the molecularly imprinted polymers. The results showed
that the combination action of functional monomer with template molecules was produced , sulfamethazine imprinted polymers u2
sing methacrylic acid as monomer had higher affinity capability than NMIP and comparatively similar structure substrate of sul2
fisoxazole (SIZ) , while SM2 imprinted polymers could specifically recognize the corresponding imprinted molecules. The static
adsorption modulus of SM2 imprinted polymer was 2821 3 mg mL - 1 and the separate factor was 31 9. Then , the prediction im2
printing principle of sulfamethazine template polymer was given. The infrared spectra of methacrylic acid (MAA) and ethylene
glycol dimethylacrylate ( EGDMA) and MIPs of SM2 were analysed. It was found that the absorbability peak of C C function
group in the IMPs turned out to be very low , but the function group of carboxylic acid in the IMPs did not markedly change. The
results showed that there were some functional groups in MIPs which could interact with the template.
Keywords Sulfamethazine ; Molecularly imprinted polymers ; Spectrum analysis ; Molecular recognition
(Received May 10 , 2006 ; accepted Aug. 20 , 2006)