Biomin PEP_IRAN 2013.pdf

Biomin ® P.E.P.
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Challenges in Poultry Nutrition
Gut microflora
Feeding the bird
Feeding the gut
microflora
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Log 10 cells/g contents
Gut Microflora: Bacterial Genera
Pathogenic
Beneficial
11
Bacteroides
Bifidobacteria
7
Lactobacilli
E. coli
2
Clostridia
Campylobacter, Salmonella
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Phytogenics Background - Essential Oils
Essential oils
Flavour properties
- Sensory effect
- Palatability effect
- Appetizing effect
Biological activities
- Anti-microbial
- Fungicidal
- Anti-viral
- Anti-oxidant
- Anti-inflammatory
- Soothing
- Anti-depressant
Essential oils help to prevent intestinal disorders
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Máthé (1996)
Effects of Essential Oils
Spice/herb
Number of identified biologically active compounds
Antioxidant Antiviral Antimicrobial
Bay 3 5 5
Cassia 3 3 3
Cayenne 9 6 8
Cumin 5 7 11
Garlic 9 5 13
Ginger 6 6 17
Oregano 14 11 19
Rosemary 12 10 19
Sage 7 - 6
Thyme 4 3 5

Biomin ® P.E.P. - Product Composition
Oregano
oil
Anis oil
Citrus
oil
- Strong antimicrobial effect
- Antioxidative efficacy
- Appetizing effect
- Antifungal
- Enhances palatability
- Antimicrobial
Milos et al. (2000), Sivropoulou et al. (1996)
Kubo et al. (2008), Çabuk et al. (2003)
Deans (1991), Van Vuuren & Viljoen (2007)
Functional carrier
- Prebiotic effect (FOS)
Xu et al. (2002), Roberfroid et al. (1998)
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Fructooligosaccharides (FOS)
• Fructose and glucose units with -glycosidic bonds
• Not digestible by endogenous enzymes
• Selectively promote beneficial bacteria, mainly in the large
intestine (“bifidogenic effect”)
• Not utilized by pathogenic microbes
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Scientific Network
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Proven Mode of Action
(Kroismayr et al., 2008)
Biomin ®
P.E.P.
Livability &
performance
Gut microflora
(nutrient
competition)
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Nutrient
digestibility
Intestinal challenge
(microbial toxins)
Immune
demand

Biomin ® P.E.P. in Broilers - Summary of Trials
Trial group
with
Control group with
Total no. of birds in
trial
%-change vs. Control
Final BW FCR PI 1
Austria P.E.P. 125 poultry - 1,024 +3.9 -7.3 +9.9
Belgium P.E.P. 125 poultry - 32,950 +4.9 -1.4
Czech Rep. P.E.P. 125 poultry - 116,160 +0.2 -5.0 +8.3
Egypt P.E.P. 125 poultry - 135 +5.0 -4.9 +10.1
France P.E.P. 125 poultry - 1,400 +1.3 -1.1 +3.7
Germany P.E.P. 125 poultry - 84,000 +4.0 -3.6 +7.9
Greece P.E.P. 125 poultry - 420 +2.6 -5.6 +7.4
Italy P.E.P. 125 poultry - 240 +0.2 -1.1 +3.0
Poland P.E.P. sol - 98,892 +1.8 -7.4 +15.1
Serbia P.E.P. 125 poultry - 1,140 +3.5 -2.1 +4.3
Serbia P.E.P. 125 poultry - 1,200 +3.6 -0.9 +4.4
South Africa P.E.P. 125 poultry - 2,016 +5.2 -9.3 +15.1
Turkey P.E.P. 1000 AGP 2 60,000 +6.3 -5.8 +11.7
Thailand P.E.P. 125 poultry - 1,800 +4.2 -3.4 +8.5
Thailand P.E.P. 1000 AGP 3 1,500 +1.5 -1.0 +0.9
India P.E.P. 125 poultry - 1,000 -0.6 -1.6 +2.0
Japan P.E.P. 1000 AGP 4 , Probiotic 136,200 +3.3 -3.0 +5.5
China P.E.P. 125 poultry 600 +3.6 -0.6 ±0.0
Taiwan P.E.P. 125 poultry 320 +5.1 -6.4 +12.0
Vietnam P.E.P. 125 poultry AGP 5 360 +0.1 -5.6 +8.2
Bolivia P.E.P. 125 poultry 900 +11.1 -2.3 +18.4
Peru P.E.P. 125 poultry AGP 2 50,000 +0.9 -2.4 +9.0
Peru P.E.P. 125 poultry AGP 3 53,440 +0.4 -0.6 +1.4
Peru P.E.P. 125 poultry AGP 4 23,680 +6.1 -2.8 +8.6
1
Production Index =Livability [%] x Live weight [kg] / age [d] / FCR x 100
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2-5
Positive Control with AGP (Antibiotic Growth Promoter): 2 Avilamycin, 3 Flavomycin, 4 Enramycin, 5 Chlortetracyclin

Biomin ® P.E.P. in Poultry
PERFORMANCE
IMPROVEMENT
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Biomin ® P.E.P. 125 poultry
France
Efficacy in poultry
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Performance in Broilers - Experimental Design
Primex S.A.S., France, Experimental Station
• 1400 day-old Ross broiler chicks
• 14 replicates (pens) in total, 100 birds/replicate (50 male, 50 female)
• 2 treatments:
Treatment Pens Birds/treatment
Control 7 700
Biomin ® P.E.P. (125 g/t) 7 700
• Diets based on wheat, corn and soybean meal
• 2 feeding phases:
Starter (crumbled)
+coccidiostat (Maxiban)
Finisher (pellets)
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Day 1 21 37

Performance in Broilers - Overall Results
Control
Biomin ® P.E.P.
(125 g/t)
Difference
Mortality (%) 2.5 1.5 -1.0
Final BW (g) 2059 A 2086 AB +27
Daily weight gain (g) 54.5 A 55.2 AB +0.7
Feed intake (g) 3669 3684 +15
FCR 1.82 b 1.80 ab -0.02
a,b Statistical significance (P

Biomin ® P.E.P. 125 poultry
USA
Efficacy in poultry
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Proven Efficacy in Broilers
Texas A&M University, USA
• 940 day-old male Cobb and Ross broiler chicks
• 10 pens (replications) per treatment with 47 birds/pen
• 2 treatments (470 birds/treatment):
1. Negative Control (no additives)
2. Control + Biomin ® P.E.P. 125 poultry (125 g/t) Day 1-21
• 3 feeding phases:
Starter Starter Finisher
Day 1 15 30 40
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Development of Weight
2.5
2.0
Body weight (kg)
2.283
2.302
1.5
1.537
1.549
1.0
0.5
0.515
0.508
0.0
Day 15 Day 30 Day 40
Control
Biomin® P.E.P.
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Texas A&M University, USA

Development of FCR
FCR
2.5
2.0
1.5
1.28
1.28
1.66
1.60
2.20
2.13
1.75
P < 0.05
1.71
1.0
0.5
0.0
Day 1-15 Day 15-30 Day 30-40 Day 1-40
Control
Biomin® P.E.P.
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Texas A&M University, USA

Biomin ® P.E.P. in Poultry
REDUCED INTESTINAL
CHALLENGE
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Biomin ® P.E.P. 125 poultry
USA
NE challenge in poultry
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Efficacy of Biomin ® P.E.P. against Necrotic Enteritis
USDA, Food & Feed Safety Research
• 3 experiments with 3 treatments (n=50 birds per treatment):
Treatment NE-challenge Biomin ® P.E.P. 125
poultry
1 - Negative Control -
2 - Positive Control + -
3 - Biomin ® P.E.P. + 125 g/t
• Experimental groups 2 and 3 received 5 x dose of Bursa-Vac TM by ocular
administration on day 14.
• Birds were orally gavaged with Clostridium perfringens (CP) once daily for
2 consecutive days with 1.5 ml of 10 7 CFU/mL.
• On day 25 the experiment was terminated and necropsy was performed.
McReynolds et al. (2009) USDA, Agricultural Research Service
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Efficacy of Biomin ® P.E.P. against Necrotic Enteritis
Lesion score
(USDA)
% birds with lesions (Uni- Gent)
2.5
2
2.15
70%
60%
59%
50%
1.5
1.33 1.28
1.2
40%
1
0.96
30%
28%
0.5
0.58
0.29 0.32 0.3
20%
10%
0
Exp. 1 Exp. 2 Exp. 3
0%
0%
No challenege
NE-challenge
NE-challenge + PEP 125 poultry
McReynolds et al. (2009) Poultry Science 88: 2075–2080
No challenege
NE-challenge
NE-challenge + PEP sol
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Biomin ® P.E.P. in Poultry
AGP REPLACEMENT
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Biomin ® P.E.P. 125 poultry
Greece
Efficacy in poultry
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Biomin ® P.E.P. in Broilers
Experimental Design
Effects of Biomin ® P.E.P. on broiler performance, digestibility, microflora and
oxidative status in comparison with an AGP
• Location: University of Athens, Greece
• Birds: 420 one-day-old male Cobb broiler chicks
• Housing: Floor pens, 3 replications/treatment
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with 35 birds/replication (105 birds/treatment)
• Treatments:
1. Negative Control (no additives)
2. Control + Biomin ® P.E.P. 125 poultry (125 g/t)
3. Control + Biomin ® P.E.P. 125 poultry (250 g/t)
4. Positive Control (Avilamycin, 25 g/t)
• Feed: Corn-soy bean meal based diets
• Feeding program:
Starter Grower Finisher
Day 1 14 28 42
Mountzouris et al. (2009) University of Athens, Greece

Biomin ® P.E.P. in Broilers - Feed Conversion Ratio
1.85
1.80
1.80 a 1.70 b 1.71 b 1.72 b
1.75
-5.6%
1.70
1.65
1.60
NC Biomin® P.E.P. (125
g/t)
Mountzouris et al. (2009) University of Athens, Greece
Biomin® P.E.P. (250
g/t)
PC (Avilamycin)
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90
Biomin ® P.E.P. in Broilers – Ileal Digestibility
80
70
60
50
40
30
20
10
P=0.08
P=0.007
0
Dry matter Crude protein Crude fat
NC Biomin® P.E.P. (125 g/t) Biomin® P.E.P. (250 g/t) PC (Avilamycin)
Mountzouris et al. (2009) University of Athens, Greece
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Biomin ® P.E.P. 1000 poultry
France
Efficacy in layer
breeders
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Efficacy in Layer Breeders - Experimental Design
Experimental farm, France (2010)
• 1800 Novogen layer breeders per group
• Laying house with floor system (½ slatted, ½ litter)
• hens assigned to 2 groups:
Group A
Group B
18-44 wk Control Biomin ® P.E.P.
45-65 wk Biomin ® P.E.P. Control
• Basal corn-SBM diet, crumb
• Duration: entire production cycle (18–65 wk of age)
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Efficacy in Layer Breeders - Results 18-65 Wk
Control
Biomin ® P.E.P.
Mortality (%) 11.1 9.2
Cum. egg no./HH 250.1 255.4
Cum. 1 st quality eggs/HH 234.7 241.5
1 st quality eggs (%) 93.8 94.6
Cum. egg mass (kg) 15049 15434
Cum. feed consumption (kg) 36210 36705
FCR 2.41 2.38
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The natural way…
Benefits of Biomin ® P.E.P.:
• Cost-efficient performance enhancer
• High profitability due to improved FCR, feed intake,
weight gains, egg production.
• No negative side effects, no withdrawal periods
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