Placental Alkaline Phosphatase (PLAP)

Placental Alkaline Phosphatase (PLAP)
Concentrated and Prediluted Rabbit Monoclonal Antibody
Control Number: 901-350-052112
ISO
9001&13485
CERTIFIED
Catalog Number: CRM 350 A, C PRM 350 AA
Description: 0.1, 1.0 ml, concentrated
6.0 ml, prediluted
Dilution: 1:300-1:600 Ready-to-use
Diluent: Van Gogh Yellow N/A
Intended Use:
For In Vitro Diagnostic Use
Summary and Explanation:
Rabbit monoclonal (PLAP) reacts with a membrane-bound isoenzyme (Regan and
Nagao type) of Placental Alkaline Phosphatase occurring in the placenta during the 3rd
trimester of gestation. This antibody is highly specific to PLAP and shows no crossreaction
with other isoenzymes of alkaline phosphatases. It is useful in the
identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive
somatic cell tumors uniformly express epithelial membrane antigen (EMA).
Principle of Procedure:
Antigen detection in tissues and cells is a multi-step immunohistochemical process.
The initial step binds the primary antibody to its specific epitope. After labeling the
antigen with a primary antibody, an enzyme labeled polymer is added to bind to the
primary antibody. The detection of the bound antibody is evidenced by a colorimetric
reaction.
Source: Rabbit Monoclonal
Species Reactivity: Human; others not tested
Clone: SP15
Isotype: Rabbit IgG
Total Protein Concentration: ~10 mg/ml. Call for lot specific Ig concentration.
Epitope/Antigen: Placental Alkaline Phosphatase (PLAP)
Cellular Localization: Cell membrane
Positive Control: Placenta or seminoma
Normal Tissue: Placenta
Abnormal Tissue: Seminoma
Known Applications:
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
Supplied As: Buffer with protein carrier and preservative.
Storage and Stability:
Store at 2ºC to 8ºC. Do not use after expiration date printed on vial. If reagents are
stored under conditions other than those specified in the package insert, they must be
verified by the user. Diluted reagents should be used promptly; any remaining reagent
should be stored at 2ºC to 8ºC.
Protocol Recommendations:
Peroxide Block:
Block for 5 minutes with Biocare's Peroxidazed 1.
Pretreatment Solution (recommended): Diva
Pretreatment Protocol:
Heat Retrieval Method:
Retrieve sections under pressure using Biocare's Decloaking Chamber, followed by a
wash in distilled water; alternatively, steam tissue sections for 45-60 minutes. Allow
solution to cool for 10 minutes then wash in distilled water.
Protein Block (Optional): Incubate for 5-10 minutes at RT with Biocare's Background
Punisher.
Primary Antibody: Incubate for 30 minutes at RT.
Protocol Recommendations Cont'd:
Probe: N/A
Polymer: Incubate for 30 minutes at RT with a polymer.
Chromogen:
Incubate for 5 minutes at RT when using Biocare's DAB - OR - Incubate for 5-7
minutes at RT when using Biocare's Warp Red.
Counterstain:
Counterstain with hematoxylin. Rinse with deionized water. Apply Tacha's Bluing
Solution for 1 minute. Rinse with deionized water.
Technical Note:
This antibody has been standardized with Biocare's MACH 2 detection system. It can
also be used on an automated staining system and with other Biocare polymer detection
kits. Use TBS buffer for washing steps.
Performance Characteristics:
The optimum antibody dilution and protocols for a specific application can vary. These
include, but are not limited to: fixation, heat-retrieval method, incubation times, tissue
section thickness and detection kit used. Due to the superior sensitivity of these unique
reagents, the recommended incubation times and titers listed are not applicable to other
detection systems, as results may vary. The data sheet recommendations and protocols
are based on exclusive use of Biocare products. Ultimately, it is the responsibility of
the investigator to determine optimal conditions. These products are tools that can be
used for interpretation of morphological findings in conjunction with other diagnostic
tests and pertinent clinical data by a qualified pathologist.
Quality Control:
Refer to CLSI Quality Standards for Design and Implementation of
Immunohistochemistry Assays; Approved Guideline-Second edition (I/LA28-A2).
CLSI Wayne, PA, USA (www.clsi.org). 2011
Precautions:
This antibody contains less than 0.1% sodium azide. Concentrations less than 0.1% are
not reportable hazardous materials according to U.S. 29 CFR 1910.1200, OSHA
Hazard communication and EC Directive 91/155/EC.
Sodium azide (NaN 3 ) used as a preservative is toxic if ingested. Sodium azide may
react with lead and copper plumbing to form highly explosive metal azides. Upon
disposal, flush with large volumes of water to prevent azide build-up in plumbing.
(Center for Disease Control, 1976, National Institute of Occupational Safety and
Health, 1976)
Specimens, before and after fixation, and all materials exposed to them should be
handled as if capable of transmitting infection and disposed of with proper precautions.
Never pipette reagents by mouth and avoid contacting the skin and mucous membranes
with reagents and specimens. If reagents or specimens come in contact with sensitive
areas, wash with copious amounts of water.
Microbial contamination of reagents may result in an increase in nonspecific staining.
Incubation times or temperatures other than those specified may give erroneous results.
The user must validate any such change. The MSDS is available upon request and is
located at http://biocare.net/support/msds/.
Troubleshooting:
Follow the antibody specific protocol recommendations according to data sheet
provided. If atypical results occur, contact Biocare's Technical Support at
1-800-542-2002.
Limitations and Warranty:
There are no warranties, expressed or implied, which extend beyond this description.
Biocare is not liable for property damage, personal injury, or economic loss caused by
this product.
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Placental Alkaline Phosphatase (PLAP)
Concentrated and Prediluted Rabbit Monoclonal Antibody
Control Number: 901-350-052112
ISO
9001&13485
CERTIFIED
References:
1. VI Shaw, et al. Utility of a selective immunohistochemical (IHC) panel in the
detection of Components of mixed germ-cell tumors (GCT) of testis. United States and
Canadian Academy of Pathology. Abstract #550. Annual Meeting, 1998.
2. Suster S, et al. Germ cell tumors of the mediastinum and testis: a comparative
immunohistochemical study of 120 cases. Hum Pathol 1998 Jul;29(7):737-42.
3. Bailey D, et al. Immunohistochemical staining of germ cell tumors and intratubularmalignant
germ cells of the testis using antibody to placental alkaline
phosphatase and a monoclonal anti-seminoma antibody. Mod Pathol 1991. Mar;4
(2):167-71.
4. Burke AP, Mostofi FK. Placental alkaline phosphatase immunohistochemistry of
intratubular malignant germ cells and associated testicular germ cell tumors. Hum
Pathol 1988 Jun;19(6):663-70.
5. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22,
Atlanta, GA. April 30, 1976 "Decontamination of Laboratory Sink Drains to Remove
Azide Salts."
6. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory
workers from occupationally Acquired Infections; Approved guideline-Third Edition
CLSI document M29-A3 Wayne, PA 2005.
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