Please find attached the program of the workshop
Please find attached the program of the workshop
Please find attached the program of the workshop
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Speakers and Lectures<br />
Day 1<br />
Piotr Bregestovski General principles and techniques <strong>of</strong><br />
electrophysiological recordings<br />
Frank Kirchh<strong>of</strong>f Differentiation <strong>of</strong> NG2 glia in health and<br />
disease<br />
Davide Ragozzino The patch clamp recording in acute slices<br />
Day 2<br />
Naoto Kawakami Intravital two-photon imaging <strong>of</strong> autoreactive T<br />
cells interacting with blood-brain barrier<br />
Irini Skaliora & Pavlos Rigas Simultaneous intracellular and<br />
field potential recordings <strong>of</strong> spontaneous cortical activity in brain<br />
slices<br />
Dimitra Thomaidou In vitro and in vivo imaging <strong>of</strong> reactive<br />
astrocytes’ dynamics<br />
Day 3<br />
Daniel Bertrand Expression and functional analysis <strong>of</strong> ionic<br />
channels in Xenopus oocytes<br />
Rebecca Matsas Stem Cells for Modeling and Treating CNS<br />
Diseases and Neurotrauma<br />
Cornelia Poulopoulou Experimental approaches for analysis <strong>of</strong><br />
function <strong>of</strong> ionic channels and its modulation by G-proteins<br />
Dimitris Davalos Microglia in <strong>the</strong> CNS: Challenges and lessons<br />
from in vivo imaging<br />
LIVE CELL IMAGING AND<br />
ELECTROPHYSIOLOGY<br />
A Theoretical and<br />
Practical Workshop<br />
Hellenic Pasteur Institute<br />
A<strong>the</strong>ns, Greece<br />
1-4 October 2013<br />
Day 4<br />
Bruno Weber In vivo imaging <strong>of</strong> cerebral metabolism and blood<br />
flow<br />
Davide Ragozzino Recording <strong>of</strong> currents from glial cells and<br />
imaging analysis <strong>of</strong> intracellular calcium or chloride ions<br />
Piotr Bregestovski Simultaneous monitoring <strong>of</strong> electrophysiological<br />
and fluorescent signals<br />
Organized by:<br />
Rebecca Matsas<br />
Dimitra Thomaidou<br />
Socrates Tzartos<br />
Marios Zouridakis<br />
FP7 REGPOT 2010-1 Neurosign 264083
Dear Meeting Attendees,<br />
We would like to welcome you to <strong>the</strong> <strong>workshop</strong> on “Live<br />
Cell Imaging and Electrophysiology”, taking place in<br />
A<strong>the</strong>ns between <strong>the</strong> 1 st and 4 th <strong>of</strong> October 2013.<br />
The aim <strong>of</strong> this <strong>workshop</strong> is to provide post graduate and<br />
post doctoral scientists with a conceptual and practical<br />
understanding <strong>of</strong> CNS live cell imaging in whole animal<br />
level, as well as electrophysiology.<br />
During <strong>the</strong> practicals, <strong>the</strong> participants will have <strong>the</strong><br />
chance to observe both live and video recorded<br />
experiments including surgical procedures for creating<br />
brain and spinal cord windows for intravital Multiphoton<br />
imaging and whole-cell and single-channel recordings,<br />
using <strong>the</strong> patch-clamp technique applied on mammalian<br />
cells.<br />
We would like to acknowledge <strong>the</strong> funding provided by<br />
<strong>the</strong> FP7 REGPOT 2010-1 EC Program “NeuroSign” that<br />
aims at <strong>the</strong> establishment <strong>of</strong> a Centre <strong>of</strong> Excellence for<br />
<strong>the</strong> study <strong>of</strong> Neurosignalling during nervous system<br />
function and dysfunction at <strong>the</strong> Hellenic Pasteur Institute.<br />
We are looking forward to seeing you and interacting<br />
with you in this <strong>workshop</strong> and we hope that it will be an<br />
educational and enjoyable experience for everyone<br />
involved.<br />
9:00 – 9:15 Welcome Introduction (on Day 1)<br />
9:15 – 10:15 1 st talk<br />
10:15-11:15 2 nd talk<br />
11:15-11:45 C<strong>of</strong>fee break<br />
11:45-12:45 3 rd talk<br />
12:45-14:00 Lunch break-poster session<br />
14:00-18:00 Practical sessions<br />
(rotation mode)<br />
On Day 3, <strong>the</strong>re will be also a 4 th<br />
talk from 14:00-15:00<br />
Practical Instructors<br />
Piotr Bregestovski<br />
Svetlana Buldakova<br />
Dafni Chroni<br />
Marios Zouridakis<br />
Pari Koutsoudaki<br />
Frank Kirchh<strong>of</strong>f<br />
Naoto Kawakami<br />
Dimitrios Davalos<br />
Evangelia Xingi<br />
Dimitra Thomaidou<br />
Electrophysiology<br />
Cranial window for brain imaging<br />
Spinal cord window for SC imaging<br />
Multiphoton imaging and analysis<br />
The Organizing Committee
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